Factors Affecting Corneal Organ Culture Contamination

Cornea ◽  
2019 ◽  
Vol 38 (7) ◽  
pp. 829-835
Author(s):  
Melvin L. H. Ling ◽  
Matthew Wells ◽  
Constantinos Petsoglou ◽  
Kehui Luo ◽  
Pierre Georges ◽  
...  
Keyword(s):  
Organ Culture ◽  
Factors Affecting ◽  
Corneal Organ Culture

Experimental Study of the Survival of Metastatic Cancer Cells in Corneal Organ Culture

2006 ◽  
Vol 47 (4) ◽  
pp. 1339 ◽  
Author(s):  
Nilanjana Deb-Joardar ◽  
Gilles Thuret ◽  
Jean-Marc Dumollard ◽  
Lena Absi ◽  
Lydia Campos-Guyotat ◽  
...  
Keyword(s):  
Experimental Study ◽  
Organ Culture ◽  
Cancer Cells ◽  
Metastatic Cancer ◽  
Corneal Organ Culture ◽  
Metastatic Cancer Cells

scholarly journals The influence of donor factors on corneal organ culture contamination

2017 ◽  
Vol 95 (7) ◽  
pp. 733-740 ◽  
Author(s):  
Anja K. Gruenert ◽  
Katja Rosenbaum ◽  
Gerd Geerling ◽  
Thomas A. Fuchsluger
Keyword(s):  
Organ Culture ◽  
Donor Factors ◽  
Corneal Organ Culture

scholarly journals An Assay System to Evaluate Riboflavin/UV-A Corneal Phototherapy Efficacy in a Porcine Corneal Organ Culture Model

Animals ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 730
Author(s):  
Anna Perazzi ◽  
Chiara Gomiero ◽  
Livio Corain ◽  
Ilaria Iacopetti ◽  
Enrico Grisan ◽  
...  
Keyword(s):  
Organ Culture ◽  
Diagnostic Methods ◽  
Assay System ◽  
Automated Image Analysis ◽  
Ocular Structure ◽  
Culture Model ◽  
Significant Difference ◽  
Corneal Organ Culture ◽  
Structure Investigations

The purpose of this study was to investigate the response of porcine corneal organ cultures to riboflavin/UV-A phototherapy in the injury healing of induced lesions. A porcine corneal organ culture model was established. Corneal alterations in the stroma were evaluated using an assay system, based on an automated image analysis method able to (i) localize the holes and gaps within the stroma and (ii) measure the brightness values in these patches. The analysis has been performed by dividing the corneal section in 24 regions of interest (ROIs) and integrating the data analysis with a “multi-aspect approach.” Three group of corneas were analyzed: healthy, injured, and injured-and-treated. Our study revealed a significant effect of the riboflavin/UV-A phototherapy in the injury healing of porcine corneas after induced lesions. The injured corneas had significant differences of brightness values in comparison to treated (p < 0.00) and healthy (p < 0.001) corneas, whereas the treated and healthy corneas showed no significant difference (p = 0.995). Riboflavin/UV-A phototherapy shows a significant effect in restoring the brightness values of damaged corneas to the values of healthy corneas, suggesting treatment restores the injury healing of corneas after lesions. Our assay system may be compared to clinical diagnostic methods, such as optical coherence tomography (OCT) imaging, for in vivo damaged ocular structure investigations.

Bacterial Lipopolysaccharides in Sterile Corneal Organ-Culture Media

Cornea ◽  
1999 ◽  
Vol 18 (1) ◽  
pp. 92-114 ◽  
Author(s):  
Angela C. Sobottka Ventura ◽  
Matthias Böhnke
Keyword(s):  
Organ Culture ◽  
Culture Media ◽  
Corneal Organ Culture ◽  
Bacterial Lipopolysaccharides

scholarly journals Time Course of Antibiotic and Antifungal Concentrations in Corneal Organ Culture

Cornea ◽  
2016 ◽  
Vol 35 (1) ◽  
pp. 127-131 ◽  
Author(s):  
Theo G. Seiler ◽  
Markus Tschopp ◽  
Stefan Zimmerli ◽  
Christoph Tappeiner ◽  
Valéry V. Wittwer ◽  
...  
Keyword(s):  
Organ Culture ◽  
Time Course ◽  
Corneal Organ Culture
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