scholarly journals Induction of Protective Immunity by Vaccination With Wild-Type Apo Superoxide Dismutase 1 in Mutant SOD1 Transgenic Mice

2010 ◽  
Vol 69 (10) ◽  
pp. 1044-1056 ◽  
Author(s):  
Shigeko Takeuchi ◽  
Noriko Fujiwara ◽  
Akemi Ido ◽  
Miki Oono ◽  
Yuki Takeuchi ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Transgenic Mice ◽  
Protective Immunity ◽  
Superoxide Dismutase 1 ◽  
Wild Type ◽  
Mutant Sod1

Ascorbic acid and CoQ10 ameliorate the reproductive ability of superoxide dismutase 1-deficient female mice†

2019 ◽  
Author(s):  
Naoki Ishii ◽  
Takujiro Homma ◽  
Jaeyong Lee ◽  
Hikaru Mitsuhashi ◽  
Ken-ichi Yamada ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Superoxide Dismutase ◽  
Coenzyme Q10 ◽  
Knockout Mice ◽  
Knockout Mouse ◽  
Superoxide Dismutase 1 ◽  
Wild Type ◽  
Positive Effects ◽  
Reproductive Ability ◽  
Ascorbic Acid Supplementation

Abstract Superoxide dismutase 1 suppresses oxidative stress within cells by decreasing the levels of superoxide anions. A dysfunction of the ovary and/or an aberrant production of sex hormones are suspected causes for infertility in superoxide dismutase 1-knockout mice. We report on attempts to rescue the infertility in female knockout mice by providing two antioxidants, ascorbic acid and/or coenzyme Q10, as supplements in the drinking water of the knockout mice after weaning and on an investigation of their reproductive ability. On the first parturition, 80% of the untreated knockout mice produced smaller litter sizes compared with wild-type mice (average 2.8 vs 7.3 pups/mouse), and supplementing with these antioxidants failed to improve these litter sizes. However, in the second parturition of the knockout mice, the parturition rate was increased from 18% to 44–75% as the result of the administration of antioxidants. While plasma levels of progesterone at 7.5 days of pregnancy were essentially the same between the wild-type and knockout mice and were not changed by the supplementation of these antioxidants, sizes of corpus luteum cells, which were smaller in the knockout mouse ovaries after the first parturition, were significantly ameliorated in the knockout mouse with the administration of the antioxidants. Moreover, the impaired vasculogenesis in uterus/placenta was also improved by ascorbic acid supplementation. We thus conclude that ascorbic acid and/or coenzyme Q10 are involved in maintaining ovarian and uterus/placenta homeostasis against insults that are augmented during pregnancy and that their use might have positive effects in terms of improving female fertility.

scholarly journals Phenotype of Transgenic Mice Carrying a Very Low Copy Number of the Mutant Human G93A Superoxide Dismutase-1 Gene Associated with Amyotrophic Lateral Sclerosis

PLoS ONE ◽  
2014 ◽  
Vol 9 (6) ◽  
pp. e99879 ◽  
Author(s):  
Jeffrey S. Deitch ◽  
Guillermo M. Alexander ◽  
Andrew Bensinger ◽  
Steven Yang ◽  
Juliann T. Jiang ◽  
...  
Keyword(s):  
Amyotrophic Lateral Sclerosis ◽  
Superoxide Dismutase ◽  
Transgenic Mice ◽  
Copy Number ◽  
Superoxide Dismutase 1 ◽  
Low Copy Number ◽  
Lateral Sclerosis

scholarly journals Mice that overexpress Cu/Zn superoxide dismutase are resistant to allergen-induced changes in airway control

2000 ◽  
Vol 279 (2) ◽  
pp. L350-L359 ◽  
Author(s):  
Gary L. Larsen ◽  
Carl W. White ◽  
Katsuyuki Takeda ◽  
Joan E. Loader ◽  
Dee Dee H. Nguyen ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Smooth Muscle ◽  
Transgenic Mice ◽  
Neural Control ◽  
Electrical Field Stimulation ◽  
Tracheal Smooth Muscle ◽  
Wild Type ◽  
Airway Control ◽  
Induced Changes

Within the respiratory epithelium of asthmatic patients, copper/zinc-containing superoxide dismutase (Cu/Zn SOD) is decreased. To address the hypothesis that lung Cu/Zn SOD protects against allergen-induced injury, wild-type and transgenic mice that overexpress human Cu/Zn SOD were either passively sensitized to ovalbumin (OVA) or actively sensitized by repeated airway exposure to OVA. Controls included nonsensitized wild-type and transgenic mice given intravenous saline or airway exposure to saline. After aerosol challenge to saline or OVA, segments of tracheal smooth muscle were obtained for in vitro analysis of neural control. In response to electrical field stimulation, wild-type sensitized mice challenged with OVA had significant increases in cholinergic reactivity. Conversely, sensitized transgenic mice challenged with OVA were resistant to changes in neural control. Stimulation of tracheal smooth muscle to elicit acetylcholine release showed that passively sensitized wild-type but not transgenic mice released more acetylcholine after OVA challenge. Function of the M2 muscarinic autoreceptor was preserved in transgenic mice. These results demonstrate that murine airways with elevated Cu/Zn SOD were resistant to allergen-induced changes in neural control.

Axonal Transport of Mutant Superoxide Dismutase 1 and Focal Axonal Abnormalities in the Proximal Axons of Transgenic Mice

1998 ◽  
Vol 5 (1) ◽  
pp. 27-35 ◽  
Author(s):  
David R. Borchelt ◽  
Philip C. Wong ◽  
Mark W. Becher ◽  
Carlos A. Pardo ◽  
Michael K. Lee ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Transgenic Mice ◽  
Axonal Transport ◽  
Superoxide Dismutase 1

Immunosuppressant FK506 does not exert beneficial effects in symptomatic G93A superoxide dismutase-1 transgenic mice

Neuroreport ◽  
2001 ◽  
Vol 12 (12) ◽  
pp. 2663-2665 ◽  
Author(s):  
Johanna M. H. Anneser ◽  
Andreas Gmerek ◽  
Jasmin Gerkrath ◽  
Gian Domenico Borasio ◽  
Rolf Heumann
Keyword(s):  
Superoxide Dismutase ◽  
Transgenic Mice ◽  
Superoxide Dismutase 1 ◽  
Beneficial Effects

scholarly journals Overexpression of Copper and Zinc Superoxide Dismutase in Transgenic Mice Prevents the Induction and Activation of Matrix Metalloproteinases after Cold Injury-Induced Brain Trauma

2000 ◽  
Vol 20 (1) ◽  
pp. 130-138 ◽  
Author(s):  
Yuiko Morita-Fujimura ◽  
Miki Fujimura ◽  
Yvan Gasche ◽  
Jean-Christophe Copin ◽  
Pak H. Chan
Keyword(s):  
Superoxide Dismutase ◽  
Matrix Metalloproteinases ◽  
Transgenic Mice ◽  
Cell Injury ◽  
Proteolytic Enzymes ◽  
Vasogenic Edema ◽  
Cold Injury ◽  
Tissue Inhibitors Of Metalloproteinases ◽  
Critical Event ◽  
Wild Type

Matrix metalloproteinases (MMPs), a family of proteolytic enzymes which degrade the extracellular matrix, are implicated in blood—brain barrier disruption, which is a critical event leading to vasogenic edema. To investigate the role of reactive oxygen species (ROS) in the expression of MMPs in vasogenic edema, the authors measured gelatinase activities before and after cold injury (CI) using transgenic mice that overexpress superoxide dismutase-1. A marked induction of pro-gelatinase B (pro-MMP-9) was seen 2 hours after CI and was maximized at 12 hours in wild-type mice. The pro-MMP-9 level was significantly lower in transgenic mice 4 hours ( P < 0.001) and 12 hours ( P < 0.05) after CI compared to wild-type mice. The activated MMP-9 was detected from 6 to 24 hours after injury. A mild induction of pro-gelatinase A (pro-MMP-2) was seen at 6 hours and was sustained until 7 days. In contrast, the activated form of MMP-2 appeared at 24 hours, was maximized at 7 days, and was absent in transgenic mice. Western blot analysis showed that the tissue inhibitors of metalloproteinases were not modified after CI. The results suggest that ROS production after CI may contribute to the induction and/or activation of MMPs and could thereby exacerbate endothelial cell injury and the development of vasogenic edema after injury.

scholarly journals Fully Metallated S134N Cu,Zn-Superoxide Dismutase Displays Abnormal Mobility and Intermolecular Contacts in Solution

2005 ◽  
Vol 280 (43) ◽  
pp. 35815-35821 ◽  
Author(s):  
Lucia Banci ◽  
Ivano Bertini ◽  
Nicola D'Amelio ◽  
Elena Gaggelli ◽  
Elisa Libralesso ◽  
...  
Keyword(s):  
Amyotrophic Lateral Sclerosis ◽  
Superoxide Dismutase ◽  
Familial Amyotrophic Lateral Sclerosis ◽  
Wild Type ◽  
Mutant Sod1 ◽  
Copper Zinc Superoxide Dismutase ◽  
Intermolecular Contacts ◽  
Nmr Methods ◽  
The Individual ◽  
Lateral Sclerosis

S134N copper-zinc superoxide dismutase (SOD1) is one of the many mutant SOD1 proteins known to cause familial amyotrophic lateral sclerosis. Earlier studies demonstrated that partially metal-deficient S134N SOD1 crystallized in filament-like arrays with abnormal contacts between the individual protein molecules. Because protein aggregation is implicated in SOD1-linked familial amyotrophic lateral sclerosis, abnormal intermolecular interactions between mutant SOD1 proteins could be relevant to the mechanism of pathogenesis in the disease. We have therefore applied NMR methods to ascertain whether abnormal contacts also form between S134N SOD1 molecules in solution and whether Cys-6 or Cys-111 plays any role in the aggregation. Our studies demonstrate that the behavior of fully metallated S134N SOD1 is dramatically different from that of fully metallated wild type SOD1 with a region of subnanosecond mobility located close to the site of the mutation. Such a high degree of mobility is usually seen only in the apo form of wild type SOD1, because binding of zinc to the zinc site normally immobilizes that region. In addition, concentration-dependent chemical shift differences were observed for S134N SOD1 that were not observed for wild type SOD1, indicative of abnormal intermolecular contacts in solution. We have here also established that the two free cysteines (6 and 111) do not play a role in this behavior.

scholarly journals Mutant superoxide dismutase-1 indistinguishable from wild-type causes ALS

2012 ◽  
Vol 21 (16) ◽  
pp. 3568-3574 ◽  
Author(s):  
Matthis Synofzik ◽  
Dario Ronchi ◽  
Isil Keskin ◽  
Ayse N. Basak ◽  
Christian Wilhelm ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Superoxide Dismutase 1 ◽  
Wild Type
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