scholarly journals Secretory Immunoglobulin A Antibody Response Is Conserved in Aged Mice following Oral Immunization with Influenza Virus Vaccine

1989 ◽  
Vol 70 (12) ◽  
pp. 3291-3296 ◽  
Author(s):  
K.-S. Chen ◽  
G. V. Quinnan
Keyword(s):  
Influenza Virus ◽  
Antibody Response ◽  
Virus Vaccine ◽  
Immunoglobulin A ◽  
Influenza Virus Vaccine ◽  
Oral Immunization ◽  
Secretory Immunoglobulin A ◽  
Aged Mice ◽  
Secretory Immunoglobulin

scholarly journals Fecal Antibodies to Cryptosporidium parvum in Healthy Volunteers

2000 ◽  
Vol 68 (9) ◽  
pp. 5068-5074 ◽  
Author(s):  
Sara M. Dann ◽  
Pablo C. Okhuysen ◽  
Bassam M. Salameh ◽  
Herbert L. DuPont ◽  
Cynthia L. Chappell
Keyword(s):  
Antibody Response ◽  
Healthy Volunteers ◽  
Cryptosporidium Parvum ◽  
Immunoglobulin A ◽  
Reactivity Index ◽  
Secretory Immunoglobulin A ◽  
Low Doses ◽  
Previous Exposure ◽  
Oocyst Excretion ◽  
Secretory Immunoglobulin

ABSTRACT This study examined the intestinal antibody response in 26 healthy volunteers challenged with Cryptosporidium parvum oocysts. Fecal extracts were assayed for total secretory immunoglobulin A (IgA) and C. parvum-specific IgA reactivity. Specific IgA reactivity was standardized to IgA concentration and expressed as a reactivity index (RI). Anti-C. parvum fecal IgA (fIgA) increased significantly in 17 of 26 (65.4%) following oocyst ingestion. Of those with detectable responses, 59, 76.5, and 94.1% were positive by days 7, 14, and 30, respectively. Volunteers receiving high challenge doses (>1,000 and 300 to 500 oocysts) had higher RIs (RI = 5.57 [P = 0.027] and RI = 1.68 [P = 0.039], respectively) than those ingesting low doses (30 to 100 oocysts; RI = 0.146). Subjects shedding oocysts and experiencing a diarrheal illness had the highest fIgA reactivity. When evaluated separately, oocyst excretion was associated with an increased fIgA response compared to nonshedders (RI = 1.679 versus 0.024, respectively; P = 0.003). However, in subjects experiencing diarrhea with or without oocyst shedding, a trend toward a higher RI (P = 0.065) was seen. Extracts positive for fecal IgA were further examined for IgA subclass. The majority of stools contained both IgA1 and IgA2, and the relative proportions did not change following challenge. Also, no C. parvum-specific IgM or IgG was detected in fecal extracts. Thus, fecal IgA to C. parvum antigens was highly associated with infection in subjects who had no evidence of previous exposure and may provide a useful tool in detecting recent infections.

Different secretory immunoglobulin A antibody responses to cholera vaccination in Swedish and Pakistani women

1980 ◽  
Vol 30 (2) ◽  
pp. 427-430
Author(s):  
A M Svennerholm ◽  
L A Hanson ◽  
J Holmgren ◽  
B S Lindblad ◽  
B Nilsson ◽  
...  
Keyword(s):  
Antibody Response ◽  
Immunoglobulin A ◽  
Antibody Responses ◽  
Secretory Immunoglobulin A ◽  
Cholera Vaccine ◽  
Intestinal Immunity ◽  
Significant Difference ◽  
Pakistani Women ◽  
Cholera Vaccination ◽  
Secretory Immunoglobulin

The capacity of subcutaneous cholera vaccination to induce an antibody response in milk and saliva was studied in lactating Swedish and Pakistani women, since secretory immunoglobulin A (SIgA) antibody responses in these secretions may reflect intestinal immunity. Before immunization, most of the Pakistani women had significant titers of specific SIgA antibodies against Vibrio cholerae lipopolysaccharide in milk, whereas only a few of the Swedish women had measurable, low titers. In the Pakistani women a single subcutaneous injection of cholera vaccine gave rise to a significant SIgA titer rise in 70% of the milk and 45% of the saliva samples. The Swedish women, on the other hand, did not respond with a significant antibody response of any immunoglobulin class in milk or saliva, either after a single or after a booster dose 14 days later. In serum, however, the vaccination induced significant titer rises, mainly of IgG antibodies, also in the Swedish women, but these rises were of lower magnitude than those in the Pakistani group. The results suggest a significant difference in the capacity of parenterally administered cholera vaccine to stimulate SIgA antibody formation in naturally primed and nonprimed individuals.

THE ANTIBODY RESPONSE IN MAN TO A QUADRIVALENT INFLUENZA VIRUS VACCINE

1955 ◽  
Vol 1 (4) ◽  
pp. 249-255
Author(s):  
Barbara K. Buchner ◽  
D. B. W. Reid ◽  
G. Dempster
Keyword(s):  
Influenza Virus ◽  
Antibody Response ◽  
Virus Vaccine ◽  
Influenza Virus Vaccine ◽  
The Other ◽  
Type B ◽  
Strain Type ◽  
Antibody Levels ◽  
Subcutaneous Inoculation

The antibody response to the subcutaneous inoculation of a single 1 ml. dose of a quadrivalent formalin-killed influenza virus egg vaccine has been measured. The vaccine used contained two A prime components and an A and a B component. Satisfactory responses were obtained two weeks after inoculation to the A and B components and to one of the A prime strains (FM1). A poor antibody response was noted to the other A prime strain incorporated in the vaccine (FW50). The highest levels were obtained with the Lee strain (Type B) which also stimulated an antibody rise to a recently isolated Type B strain. Antibody levels were maintained for at least 12 weeks. Treatment of the sera with RDE was found to influence the results obtained with the FM1 strain used.

scholarly journals Responses of volunteers to inactivated influenza virus vaccines

1981 ◽  
Vol 86 (1) ◽  
pp. 1-16 ◽  
Author(s):  
R. Jennings ◽  
C. W. Potter ◽  
P. M. O. Massey ◽  
B. I. Duerden ◽  
J. Martin ◽  
...  
Keyword(s):  
Influenza Virus ◽  
New Jersey ◽  
Antibody Response ◽  
Surface Antigen ◽  
Virus Vaccine ◽  
Influenza Virus Vaccine ◽  
Systemic Reactions ◽  
Antigen Vaccine ◽  
Different Types ◽  
Aqueous Surface

SUMMARYThree different types of bivalent influenza virus vaccine, a whole virus, an aqueous-surface-antigen vaccine and an adsorbed-surface-antigen vaccine were tested at three dosage levels in volunteers primed with respect to only one of the haemagglutinin antigens present in the vaccines.The local and systemic reactions to all three vaccine types were mild in nature and, following first immunization, the aqueous-surface-antigen vaccine was the least reactogenic. The serum haemagglutination-inhibiting antibody response to the A/Victoria/75 component of the vaccines, to which the volunteer population was primed, was greatest following immunization with the aqueous-surface-antigen vaccine; the greatest antibody response to the A/New Jersey/76 component of the vaccines was observed following immunization with whole virus vaccine.

ANTIBODY RESPONSE TO INFLUENZA-VIRUS VACCINE IN CHILDREN WITH NEPHROSIS: EFFECT OF CORTISONE

PEDIATRICS ◽  
1959 ◽  
Vol 23 (1) ◽  
pp. 54-62
Author(s):  
Calvin M. Kunin ◽  
Robert Schwartz ◽  
Sumner Yaffe ◽  
John Knapp ◽  
Francis X. Fellers ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Nephrotic Syndrome ◽  
Antibody Response ◽  
Virus Vaccine ◽  
Influenza A ◽  
Serum Levels ◽  
Influenza Virus Vaccine ◽  
Inactive Disease ◽  
Strain Component ◽  
Adrenogenital Syndrome

Polyvalent influenza-virus vaccine was administered to 55 children; 44 of these children were in various stages of nephrotic syndrome, including 17 under treatment with adrenocortical hormones; 5 were children with the adrenogenital syndrome receiving replacement cortisone therapy, and 6 were normal controls. Antibody response, as measured by the hemagglutination-inhibition test 2 weeks following a single subcutaneous dose of vaccine, was not significantly different in children with active or inactive disease, and appeared to be similar in those receiving hormone therapy and in those who were not. The serum levels of gamma-globulin were lower in children with active nephrotic syndrome, but this did not affect either the prevaccination levels of influenza A prime antibody or the response to the vaccine. No change in the status of activity of nephrosis occurred during the period of immunization or immediately thereafter, even in patients who had moderate febrile reactions to the vaccine. The failure of any of the groups to respond serologically to the Asian strain component of the vaccine is discussed.

scholarly journals Immunostimulant Patch Containing Heat-Labile Enterotoxin from Escherichia coli Enhances Immune Responses to Injected Influenza Virus Vaccine through Activation of Skin Dendritic Cells

2003 ◽  
Vol 77 (9) ◽  
pp. 5218-5225 ◽  
Author(s):  
Mimi Guebre-Xabier ◽  
Scott A. Hammond ◽  
Diane E. Epperson ◽  
Jianmei Yu ◽  
Larry Ellingsworth ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Influenza Virus ◽  
Immune Responses ◽  
Virus Vaccine ◽  
Immunoglobulin A ◽  
Unmet Need ◽  
The Elderly ◽  
Influenza Virus Vaccine ◽  
Interleukin 4 ◽  
Heat Labile

ABSTRACT Vaccine strategies, such as influenza virus vaccination of the elderly, are highly effective at preventing disease but provide protection for only the responding portion of the vaccinees. Adjuvants improve the magnitude and rates of responses, but their potency must be attenuated to minimize side effects. Topical delivery of strong adjuvants such as heat-labile enterotoxin from Escherichia coli (LT) induces potent immune responses. We hypothesized that LT delivered alone in an immunostimulating (LT-IS) patch placed on the skin at the site of injection could augment the immune response to injected vaccines. This was based on the observation that topically applied LT induces migration of activated antigen-presenting cells (APCs) from the skin to the proximal draining lymph node (DLN), and that APCs loaded with antigen by injection in the same anatomical region also migrate to the same DLN. We observed that when influenza virus vaccine is injected and an LT-IS patch is placed to target the same DLN, the influenza virus antibody response is enhanced. Similarly, influenza virus-specific T cells isolated from the lungs show increased levels of gamma interferon and interleukin-4 production. An LT-IS patch placed near an injected vaccine also leads to increased levels of hemagglutination inhibition titers, enhanced mucosal immunoglobulin A responses, and enhanced antigen presentation. Although the mechanisms by which an LT-IS patch exerts its enhancing effects need further study, the enhanced immune responses, ability to safely use potent adjuvants, and simplicity of LT-IS patch application address an important unmet need and provide a new immune enhancement strategy.

Sign in / Sign up

Export Citation Format

Share Document