scholarly journals Evidence for Related Virulence Sequences in Plasmids of Salmonella dublin and Salmonella typhimurium

Microbiology ◽  
1985 ◽  
Vol 131 (7) ◽  
pp. 1815-1823 ◽  
Author(s):  
G. D. BAIRD ◽  
E. J. MANNING ◽  
P. W. JONES
Keyword(s):  
Salmonella Typhimurium ◽  
Salmonella Dublin

scholarly journals The immunity produced by a rough Salmonella dublin variant against Salmonella typhimurium and Salmonella choleraesuis infection in guinea-pigs

1966 ◽  
Vol 64 (3) ◽  
pp. 357-359 ◽  
Author(s):  
H. Williams Smith ◽  
Sheila Halls
Keyword(s):  
Salmonella Typhimurium ◽  
Guinea Pigs ◽  
Harmful Effect ◽  
Salmonella Dublin ◽  
Salmonella Choleraesuis

Vaccination with a rough variant of Salmonella dublin had no observable harmful effect on guinea-pigs. It conferred a good immunity against S. typhimurium and S. choleraesuis var kunzendorf infections.

scholarly journals Evaluation of WGS-subtyping methods for epidemiological surveillance of foodborne salmonellosis.

2020 ◽  
Author(s):  
Manal Mohammed ◽  
Salina Thapa
Keyword(s):  
Salmonella Typhimurium ◽  
Multilocus Sequence Typing ◽  
Bacterial Infections ◽  
Outbreak Investigation ◽  
Whole Genome Sequence ◽  
Epidemiological Surveillance ◽  
Whole Genome ◽  
Salmonella Dublin ◽  
Source Of Infection ◽  
Investigation Methods

Abstract Background: Salmonellosis is one of the most common foodborne diseases worldwide. Although human infection by non-typhoidal Salmonella (NTS) enterica subspecies enterica is associated primarily with a self-limiting diarrhoeal illness, invasive bacterial infections (such as septicaemia, bacteraemia and meningitis) were also reported. Human outbreaks of NTS were reported in several countries all over the world including developing as well as high-income countries. Conventional laboratory methods such as pulsed field gel electrophoresis (PFGE) do not display adequate discrimination and have their limitations in epidemiological surveillance. It is therefore very crucial to use accurate, reliable and highly discriminative subtyping methods for epidemiological characterisation and outbreak investigation. Methods: Here, we used different whole genome sequence (WGS)-based subtyping methods for retrospective investigation of two different outbreaks of Salmonella Typhimurium and Salmonella Dublin that occurred in 2013 in UK and Ireland respectively. Results: Single nucleotide polymorphism (SNP)-based cluster analysis of Salmonella Typhimurium genomes revealed well supported clades, that were concordant with epidemiologically defined outbreak and confirmed the source of outbreak is due to consumption of contaminated mayonnaise. SNP-analyses of Salmonella Dublin genomes confirmed the outbreak however the source of infection could not be determined. The core genome multilocus sequence typing (cgMLST) was discriminatory and separated the outbreak strains of Salmonella Dublin from the non-outbreak strains that were concordant with the epidemiological data however cgMLST could neither discriminate between the outbreak and non-outbreak strains of Salmonella Typhimurium nor confirm that contaminated mayonnaise is the source of infection, On the other hand, other WGS-based subtyping methods including multilocus sequence typing (MLST), ribosomal MLST (rMLST), whole genome MLST (wgMLST), clustered regularly interspaced short palindromic repeats (CRISPRs), prophage sequence profiling, antibiotic resistance profile and plasmid typing methods were less discriminatory and could not confirm the source of the outbreak. Conclusions: Foodborne salmonellosis is an important concern for public health therefore, it is crucial to use accurate, reliable and highly discriminative subtyping methods for epidemiological surveillance and outbreak investigation. In this study, we showed that SNP-based analyses do not only have the ability to confirm the occurrence of the outbreak but also to provide definitive evidence of the source of the outbreak in real-time.

Survival of Salmonella dublin and Salmonella typhimurium in Lebanon Bologna

1975 ◽  
Vol 38 (3) ◽  
pp. 150-154 ◽  
Author(s):  
J. L. SMITH ◽  
S. A. PALUMBO ◽  
J. C. KISSINGER ◽  
C. N. HUHTANEN
Keyword(s):  
Salmonella Typhimurium ◽  
Starter Culture ◽  
Viable Cell ◽  
Salmonella Dublin ◽  
Aging Period ◽  
Cell Counts ◽  
Natural Flora

Survival and destruction of Salmonella dublin and S. typhimurium added to Lebanon bologna was studied during manufacture of this sausage. During the aging period of salted cubed beef at 5 C, viable cell counts of S. dublin did not change over a 10-day period. Cell counts of S. Dublin were reduced 3 to 4 log cycles during the 4-day fermentation at 35 C; further reduction in the viable Salmonella count occurred during mellowing of the bolognas at 5 C. The number of S. typhimurium was usually reduced to an undetectible level before the end of the fermentation. Salmonella typhimurium was consistently more sensitive to the acid conditions of Lebanon bologna than was S. dublin. Introduction of an optional cooking step indicated that heating of bolognas to 51.7 C or above led to destruction of salmonellae. Unaged beef which was not inoculated with starter culture did not ferment and there was very little reduction in numbers of added salmonellae. Salmonellae were destroyed more rapidly in Lebanon bologna made from unaged beef with starter culture than in bologna made from aged beef (natural flora fermentation). Smoking also appeared to contribute to destruction of salmonellae. Four commercial Lebanon bolognas were tested for the presence of salmonellae but none were detected.

Comparison of the environmental survival characteristics of Salmonella Dublin and Salmonella Typhimurium

2012 ◽  
Vol 159 (3-4) ◽  
pp. 509-514 ◽  
Author(s):  
Miranda J. Kirchner ◽  
Ernesto Liebana ◽  
Ian McLaren ◽  
Felicity A. Clifton-Hadley ◽  
Andrew D. Wales ◽  
...  
Keyword(s):  
Salmonella Typhimurium ◽  
Salmonella Dublin ◽  
Environmental Survival

scholarly journals Interaction of Salmonella choleraesuis, Salmonella dublin and Salmonella typhimurium with porcine and bovine terminal ileum in vivo

Microbiology ◽  
1999 ◽  
Vol 145 (9) ◽  
pp. 2431-2441 ◽  
Author(s):  
Alex J. Bolton ◽  
Michael P. Osborne ◽  
Tim S. Wallis ◽  
John Stephen
Keyword(s):  
Salmonella Typhimurium ◽  
Terminal Ileum ◽  
Salmonella Dublin ◽  
Salmonella Choleraesuis

scholarly journals Evaluation of WGS-subtyping methods for epidemiological surveillance of foodborne salmonellosis.

2020 ◽  
Author(s):  
Manal Mohammed ◽  
Salina Thapa
Keyword(s):  
Salmonella Typhimurium ◽  
Multilocus Sequence Typing ◽  
Bacterial Infections ◽  
Rapid Development ◽  
Outbreak Investigation ◽  
Whole Genome Sequence ◽  
Epidemiological Surveillance ◽  
Whole Genome ◽  
Salmonella Dublin ◽  
Investigation Methods

Abstract Background: Salmonellosis is one of the most common foodborne diseases worldwide. Although human infection by non-typhoidal Salmonella (NTS) enterica subspecies enterica is associated primarily with a self-limiting diarrhoeal illness, invasive bacterial infections (such as septicaemia, bacteraemia and meningitis) were also reported. Human outbreaks of NTS were reported in several countries all over the world including developing as well as high-income countries. Conventional laboratory methods such as pulsed field gel electrophoresis (PFGE) do not display adequate discrimination and have their limitations in epidemiological surveillance. It is therefore very crucial to use accurate, reliable and highly discriminative subtyping methods for epidemiological characterisation and outbreak investigation. Methods: Here, we used different whole genome sequence (WGS)-based subtyping methods for retrospective investigation of two different outbreaks of Salmonella Typhimurium and Salmonella Dublin that occurred in 2013 in UK and Ireland respectively. Results: The core genome multilocus sequence typing (cgMLST) was discriminatory and separated the outbreak strains of Salmonella Dublin from the non-outbreak strains that were concordant with the epidemiological data however cgMLST could not discriminate between outbreak and non-outbreak strains of Salmonella Typhimurium, On the other hand, other WGS-based subtyping methods including multilocus sequence typing (MLST), ribosomal MLST (rMLST), whole genome MLST (wgMLST), clustered regularly interspaced short palindromic repeats (CRISPRs), prophage sequence profiling, antibiotic resistance profile and plasmid typing methods were less discriminatory. Conclusions: Foodborne salmonellosis is an important concern for public health therefore, it is crucial to use accurate, reliable and highly discriminative subtyping methods for epidemiological surveillance and outbreak investigation. The rapid development of next-generation sequencing (NGS) technology and bioinformatics tools have enabled WGS of any bacterial strain feasible. Various typing tools have been proposed by using WGS data but currently, the adoption of WGS-based methods have proved to be difficult due to lack of standardization. There are many layers on obtaining WGS data and there is need of standardization from the type of sequencers used to the bioinformatics analysis. Therefore, the emerging genetic analysis techniques should be combined with conventional phenotypic and molecular methods for routine surveillance and outbreak investigation until the WGS-based methods can be fully exploited, improved and standardized.

scholarly journals Characterization of intestinal invasion by Salmonella typhimurium and Salmonella dublin and effect of a mutation in the invH gene.

1995 ◽  
Vol 63 (7) ◽  
pp. 2743-2754 ◽  
Author(s):  
P R Watson ◽  
S M Paulin ◽  
A P Bland ◽  
P W Jones ◽  
T S Wallis
Keyword(s):  
Salmonella Typhimurium ◽  
Salmonella Dublin
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