scholarly journals The Relationship Between Activity and Cell-wall Permeability in Dried Baker's Yeast

1961 ◽  
Vol 25 (1) ◽  
pp. 87-95 ◽  
Author(s):  
L. I. K. EBBUTT
2021 ◽  
Author(s):  
Yanrong Huang ◽  
Sushil Dhital ◽  
Feitong Liu ◽  
Xiong Fu ◽  
Qiang Huang ◽  
...  

Processing induced structural changes of whole foods on regulation of colonic fermentation rate and microbiota composition are least understood and often overlooked. In the present study, intact cotyledon cells from...


2003 ◽  
Vol 69 (12) ◽  
pp. 7019-7027 ◽  
Author(s):  
Ivana Sokolovská ◽  
Raoul Rozenberg ◽  
Christophe Riez ◽  
Paul G. Rouxhet ◽  
Spiros N. Agathos ◽  
...  

ABSTRACT The influence of the carbon source on cell wall properties was analyzed in an efficient alkane-degrading strain of Rhodococcus erythropolis (strain E1), with particular focus on the mycolic acid content. A clear correlation was observed between the carbon source and the mycolic acid profiles as estimated by high-performance liquid chromatography and mass spectrometry. Two types of mycolic acid patterns were observed after growth either on saturated linear alkanes or on short-chain alkanoates. One type of pattern was characterized by the lack of odd-numbered carbon chains and resulted from growth on linear alkanes with even numbers of carbon atoms. The second type of pattern was characterized by mycolic acids with both even- and odd-numbered carbon chains and resulted from growth on compounds with odd-numbered carbon chains, on branched alkanes, or on mixtures of different compounds. Cellular short-chain fatty acids were twice as abundant during growth on a branched alkane (pristane) as during growth on acetate, while equal amounts of mycolic acids were found under both conditions. More hydrocarbon-like compounds and less polysaccharide were exposed at the cell wall surface during growth on alkanes. Whatever the substrate, the cells had the same affinity for aqueous-nonaqueous solvent interfaces. By contrast, bacteria displayed completely opposite susceptibilities to hydrophilic and hydrophobic antibiotics and were found to be strongly stained by hydrophobic dyes after growth on pristane but not after growth on acetate. Taken together, these data show that the cell wall composition of R. erythropolis E1 is influenced by the nutritional regimen and that the most marked effect is a radical change in cell wall permeability.


2018 ◽  
Vol 81 (4) ◽  
pp. 670-676 ◽  
Author(s):  
MOHAMMAD HADI AAZAMI ◽  
MOHAMMAD HASAN FATHI NASRI ◽  
MOHSEN MOJTAHEDI ◽  
SHAHLA ROUDBAR MOHAMMADI

ABSTRACT The aim of this study was to evaluate the ability of heat-killed baker's yeast (HKBY), the cell wall of baker's yeast (CWBY), and cell wall (1→3)-β-d-glucan of baker's yeast (BGBY) to bind aflatoxin B1 (AFB1) in phosphate-buffered saline (PBS) spiked with 0.5 μg/mL AFB1. Baker's yeast (Saccharomyces cerevisiae) was heat killed by autoclaving at 121°C for 10 min. The cell wall was physically extracted, and (1→3)-β-d-glucan was extracted by a modified method. The concentration of AFB1 was determined by high-performance liquid chromatography after exposure to binders for three contact times, 30 min, 5 h, and 24 h, at room temperature. AFB1 binding by HKBY, CWBY, and BGBY was 6.30 to 46.34%. The lowest binding capacity was found for HKBY with a contact time of 30 min, and the highest binding capacity was found for BGBY with a contact time of 24 h. Among binders, CWBY had the highest binder-AFB1 complex stability during washing with PBS, and the lowest stability was found for HKBY complexes. Results of this study indicated that BGBY was the most effective binder, and more exposure to BGBY removes more AFB1 from PBS.


2002 ◽  
Vol 277 (40) ◽  
pp. 37567-37572 ◽  
Author(s):  
Harald Engelhardt ◽  
Christian Heinz ◽  
Michael Niederweis

2016 ◽  
Vol 15 (2) ◽  
pp. 114-121
Author(s):  
Monika Bardáčová ◽  
Marína Maglovski ◽  
Zuzana Gregorová ◽  
Yevheniia Konotop ◽  
Miroslav Horník ◽  
...  

AbstractCell walls represent the first barrier that can prevent the entrance of toxic heavy metals into plants. The composition and the flexibility of the cell wall are regulated by different enzymes. The ß-1,3-glucanases control the degradation of the polysaccharide callose as a flexible regulation mechanism of cell wall permeability and/or its ability to bind metals under stress conditions. The profile and activity of ß-1,3-glucanases in the presence of heavy metals, however, has rarely been studied. Here we studied these enzymes in four soybean varieties (Glycine max) grown in the presence of cadmium ions. These analyses revealed three acidic and one basic enzyme isoforms in each soybean variety, but only two of the acidic isoforms in the variety Moravians were substantially responsive to the presence of Cd2+. Since the responses of certain glucanases were detected mainly in the varieties sensitive to metal and accumulating high amounts of metals, we assume their role in the defense rather than strategic metal sequestration.


2021 ◽  
Vol 19 (6) ◽  
pp. 49-53
Author(s):  
Tatyana Vasilyevna Ryabtseva ◽  
Denis Alexandrovich Makarevich ◽  
Evgeny Mikhailovich Ermola

Science ◽  
1956 ◽  
Vol 124 (3215) ◽  
pp. 272-273
Author(s):  
G. Falcone ◽  
Walter J. Nickerson

Sign in / Sign up

Export Citation Format

Share Document