scholarly journals Alkalibacillus flavidus sp. nov., isolated from a marine solar saltern

2010 ◽  
Vol 60 (2) ◽  
pp. 434-438 ◽  
Author(s):  
Jung-Hoon Yoon ◽  
So-Jung Kang ◽  
Yong-Taek Jung ◽  
Mi-Hwa Lee ◽  
Tae-Kwang Oh
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Sequence Similarity ◽  
Diaminopimelic Acid ◽  
The Yellow Sea ◽  
Rrna Gene ◽  
Solar Saltern ◽  
Marine Solar Saltern ◽  
Phenotypic Properties ◽  
Diamino Acid

A Gram-stain-positive, motile, rod-shaped bacterial strain, ISL-17T, was isolated from a marine solar saltern of the Yellow Sea, Korea, and its taxonomic position was investigated by means of a polyphasic study. Strain ISL-17T grew optimally at pH 8.5–9.0, at 37 °C and in the presence of approximately 10 % (w/v) NaCl. It contained meso-diaminopimelic acid as the diagnostic diamino acid in the peptidoglycan, MK-7 as the predominant menaquinone and iso-C15 : 0, anteiso-C15 : 0 and iso-C16 : 0 as the major fatty acids. The DNA G+C content was 48.1 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain ISL-17T fell within the genus Alkalibacillus, clustering with Alkalibacillus salilacus BH163T with a bootstrap resampling value of 100 %. Strain ISL-17T exhibited 98.2 % 16S rRNA gene sequence similarity to A. salilacus BH163T and 95.8–96.5 % similarity to the type strains of the other Alkalibacillus species. The mean DNA–DNA relatedness value between strain ISL-17T and A. salilacus KCTC 3916T was 19 %. The phenotypic properties of strain ISL-17T, together with its phylogenetic and genetic distinctiveness, enable this strain to be differentiated from recognized Alkalibacillus species. On the basis of phenotypic, phylogenetic and genetic data, strain ISL-17T represents a novel species within the genus Alkalibacillus, for which the name Alkalibacillus flavidus sp. nov. is proposed; the type strain is ISL-17T (=KCTC 13258T =CCUG 56753T).

scholarly journals Marinobacter salicampi sp. nov., isolated from a marine solar saltern in Korea

2007 ◽  
Vol 57 (9) ◽  
pp. 2102-2105 ◽  
Author(s):  
Jung-Hoon Yoon ◽  
Mi-Hwa Lee ◽  
So-Jung Kang ◽  
Tae-Kwang Oh
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Sequence Similarity ◽  
The Yellow Sea ◽  
Rrna Gene ◽  
The Novel ◽  
Solar Saltern ◽  
Marine Solar Saltern ◽  
Phenotypic Properties ◽  
Type Strains

A Gram-negative, motile, rod-shaped, Marinobacter-like bacterial strain, ISL-40T, was isolated from a marine solar saltern of the Yellow Sea in Korea. The taxonomic position of the novel strain was investigated using a polyphasic approach. Strain ISL-40T grew optimally at pH 7.0–8.0 and at 30 °C. It contained Q-9 as the predominant ubiquinone. The major fatty acids were C16 : 0, C16 : 1 ω7c and/or iso-C15 : 0 2-OH and 10-methyl C16 : 0. The DNA G+C content was 58.1 mol%. A phylogenetic analysis based on 16S rRNA gene sequences showed that strain ISL-40T belongs to the genus Marinobacter. Strain ISL-40T exhibited 16S rRNA gene sequence similarity values of 93.5–96.4 % to the type strains of recognized Marinobacter species. The differential phenotypic properties and phylogenetic distinctiveness of strain ISL-40T revealed that it is separate from recognized Marinobacter species. On the basis of phenotypic, phylogenetic and genetic data, therefore, strain ISL-40T represents a novel species of the genus Marinobacter, for which the name Marinobacter salicampi sp. nov. is proposed. The type strain is ISL-40T (=KCTC 12972T=CCUG 54357T).

scholarly journals Microbulbifer celer sp. nov., isolated from a marine solar saltern of the Yellow Sea in Korea

2007 ◽  
Vol 57 (10) ◽  
pp. 2365-2369 ◽  
Author(s):  
Jung-Hoon Yoon ◽  
Seo-Youn Jung ◽  
So-Jung Kang ◽  
Tae-Kwang Oh
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Yellow Sea ◽  
Sequence Similarity ◽  
The Yellow Sea ◽  
Rrna Gene ◽  
Solar Saltern ◽  
Marine Solar Saltern ◽  
Phenotypic Properties ◽  
Type Strains

A Gram-negative, non-motile, rod-shaped, Microbulbifer-like bacterial strain, ISL-39T, was isolated from a marine solar saltern of the Yellow Sea in Korea and was subjected to a polyphasic taxonomic investigation. Strain ISL-39T grew optimally at pH 7.0–8.0 and 37 °C. It contained Q-8 as the predominant ubiquinone and iso-C15 : 0, C16 : 0 and iso-C17 : 0 as the major fatty acids. The DNA G+C content was 57.7 mol%. A phylogenetic analysis based on 16S rRNA gene sequences showed that strain ISL-39T belonged to the genus Microbulbifer. Strain ISL-39T exhibited 16S rRNA gene sequence similarity values of 94.7–97.5 % with respect to the type strains of four recognized Microbulbifer species. DNA–DNA relatedness data and the differential phenotypic properties and phylogenetic distinctiveness of ISL-39T make this strain distinguishable from the recognized Microbulbifer species. On the basis of the phenotypic, phylogenetic and genetic data, strain ISL-39T represents a novel species of the genus Microbulbifer, for which the name Microbulbifer celer sp. nov. is proposed. The type strain is ISL-39T (=KCTC 12973T=CCUG 54356T).

scholarly journals Salegentibacter salarius sp. nov., isolated from a marine solar saltern

2007 ◽  
Vol 57 (12) ◽  
pp. 2738-2742 ◽  
Author(s):  
Jung-Hoon Yoon ◽  
Seo-Youn Jung ◽  
So-Jung Kang ◽  
Yong-Taek Jung ◽  
Tae-Kwang Oh
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Sequence Similarity ◽  
The Yellow Sea ◽  
Rrna Gene ◽  
Solar Saltern ◽  
Marine Solar Saltern ◽  
Phenotypic Properties ◽  
Bacterium Strain ◽  
Type Strains

A Gram-negative, non-motile, rod-shaped bacterium, strain ISL-6T, phenotypically resembling members of the genus Salegentibacter, was isolated from a marine solar saltern of the Yellow Sea in Korea and subjected to a polyphasic taxonomic investigation. Strain ISL-6T grew optimally at pH 7.0–8.0 and 30 °C and in the presence of 8 % (w/v) NaCl. It contained MK-6 as the predominant menaquinone. The major fatty acids were iso-C15 : 0 and anteiso-C15 : 0. The DNA G+C content was 37.5 mol%. A phylogenetic analysis based on 16S rRNA gene sequences showed that strain ISL-6T belonged to the genus Salegentibacter. Strain ISL-6T exhibited 16S rRNA gene sequence similarity values of 92.0–98.6 % with respect to the type strains of recognized Salegentibacter species. Low DNA–DNA relatedness values, differential phenotypic properties and phylogenetic distinctiveness demonstrated that strain ISL-6T is distinguishable from the recognized Salegentibacter species. Therefore strain ISL-6T represents a novel species of the genus Salegentibacter, for which the name Salegentibacter salarius sp. nov. is proposed. The type strain is ISL-6T (=KCTC 12974T =CCUG 54355T).

scholarly journals Idiomarina salinarum sp. nov., isolated from a marine solar saltern in Korea

2007 ◽  
Vol 57 (11) ◽  
pp. 2503-2506 ◽  
Author(s):  
Jung-Hoon Yoon ◽  
Seo-Youn Jung ◽  
Yong-Taek Jung ◽  
Tae-Kwang Oh
Keyword(s):  
Fatty Acids ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Strain ◽  
Sequence Similarity ◽  
The Yellow Sea ◽  
Rrna Gene ◽  
Solar Saltern ◽  
Marine Solar Saltern ◽  
Phenotypic Properties

A Gram-negative, motile, rod-shaped, Idiomarina-like bacterial strain, ISL-52T, was isolated from a marine solar saltern of the Yellow Sea in Korea and was subjected to a polyphasic taxonomic investigation. Strain ISL-52T grew optimally at pH 7.0–8.0 and at 30–37 °C. It contained Q-8 as the predominant ubiquinone. The major fatty acids (>10 % of total fatty acids) were iso-C15 : 0, iso-C17 : 0 and iso-C17 : 1 ω9c. The DNA G+C content was 53.9 mol%. A phylogenetic analysis based on 16S rRNA gene sequences showed that strain ISL-52T fell within the genus Idiomarina, joining the type strain of Idiomarina homiensis at a bootstrap resampling value of 100 %. Strain ISL-52T exhibited 16S rRNA gene sequence similarity values of 94.9–96.7 % with respect to the type strains of eight recognized Idiomarina species. The differential phenotypic properties of ISL-52T, together with its phylogenetic distinctiveness, demonstrated that this strain is distinguishable from the recognized Idiomarina species. On the basis of phenotypic, phylogenetic and genetic data, therefore, strain ISL-52T represents a novel species of the genus Idiomarina, for which the name Idiomarina salinarum sp. nov. is proposed. The type strain is ISL-52T (=KCTC 12971T=CCUG 54359T).

Reclassification of Marinococcus albus Hao et al. 1985 as Salimicrobium album gen. nov., comb. nov. and Bacillus halophilus Ventosa et al. 1990 as Salimicrobium halophilum comb. nov., and description of Salimicrobium luteum sp. nov.

2007 ◽  
Vol 57 (10) ◽  
pp. 2406-2411 ◽  
Author(s):  
Jung-Hoon Yoon ◽  
So-Jung Kang ◽  
Tae-Kwang Oh
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
New Genus ◽  
Sequence Similarity ◽  
Phylogenetic Analyses ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Phenotypic Properties ◽  
Separate Species ◽  
Diamino Acid

A Gram-positive, non-motile, coccoid-shaped, non-spore-forming halophilic bacterial strain, BY-5T, was isolated from a marine solar saltern in Korea and its taxonomic position was investigated by using a polyphasic approach. The novel strain grew optimally at 37 °C and in the presence of 10 % (w/v) NaCl. Strain BY-5T had meso-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan, MK-7 as the predominant menaquinone and anteiso-C15 : 0, iso-C15 : 0, anteiso-C17 : 0 and iso-C17 : 0 as the major fatty acids. The DNA G+C content was 47.9 mol%. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain BY-5T formed a coherent cluster with Bacillus halophilus and Marinococcus albus. Strain BY-5T exhibited 16S rRNA gene sequence similarity values of 98.7 and 97.4 % to the type strains of B. halophilus and M. albus, respectively. Strain BY-5T was distinguished from B. halophilus and M. albus by several phenotypic properties and DNA–DNA relatedness data. On the basis of the combined chemotaxonomic and phylogenetic data, it is proposed that M. albus, B. halophilus and strain BY-5T should be placed in a new genus as three separate species. Marinococcus albus and Bacillus halophilus are reclassified in a new genus, Salimicrobium gen. nov., as Salimicrobium album comb. nov. and Salimicrobium halophilum comb. nov., respectively. The type species of the new genus is Salimicrobium album. Strain BY-5T (=KCTC 3989T=CIP 108918T) is placed in the genus Salimicrobium as a novel species Salimicrobium luteum sp. nov.

scholarly journals Virgibacillus campisalis sp. nov., from a marine solar saltern

2012 ◽  
Vol 62 (2) ◽  
pp. 347-351 ◽  
Author(s):  
Soo-Young Lee ◽  
Chul-Hyung Kang ◽  
Tae-Kwang Oh ◽  
Jung-Hoon Yoon
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Sequence Similarity ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Solar Saltern ◽  
Marine Solar Saltern ◽  
Gram Staining ◽  
A Cell ◽  
Type Strains

A Gram-staining-variable, motile, endospore-forming and rod-shaped bacterial strain, IDS-20T, was isolated from a marine solar saltern in Korea and subjected to a polyphasic taxonomic investigation. Strain IDS-20T grew optimally at 37 °C, at pH 7.5–8.0 and in the presence of 4–5 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain IDS-20T belongs to the genus Virgibacillus. Strain IDS-20T exhibited 93.4–96.6 % 16S rRNA gene sequence similarity to the type strains of species of the genus Virgibacillus. Strain IDS-20T had MK-7 as the predominant menaquinone and a cell-wall peptidoglycan based on meso-diaminopimelic acid. The major fatty acids were anteiso-C15 : 0 and anteiso-C17 : 0 and major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and two unidentified phospholipids. The DNA G+C content was 39.5 mol%. The phylogenetic distinctiveness and differential phenotypic characteristics of strain IDS-20T demonstrated that this strain can be distinguished from recognized species of the genus Virgibacillus. On the basis of the data presented, strain IDS-20T represents a novel species of the genus Virgibacillus, for which the name Virgibacillus campisalis sp. nov. is proposed. The type strain is IDS-20T ( = KCTC 13727T  = CCUG 59308T).

scholarly journals Jeotgalibacillus salarius sp. nov., isolated from a marine saltern, and reclassification of Marinibacillus marinus and Marinibacillus campisalis as Jeotgalibacillus marinus comb. nov. and Jeotgalibacillus campisalis comb. nov., respectively

2010 ◽  
Vol 60 (1) ◽  
pp. 15-20 ◽  
Author(s):  
Jung-Hoon Yoon ◽  
So-Jung Kang ◽  
Peter Schumann ◽  
Tae-Kwang Oh
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Strain ◽  
Sequence Similarity ◽  
The Yellow Sea ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Phenotypic Properties ◽  
Diamino Acid ◽  
Type Strains

A Gram-variable, motile and rod-shaped bacterial strain, ASL-1T, was isolated from a marine saltern located on the coast of the Yellow Sea, Korea. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain ASL-1T clustered with Jeotgalibacillus alimentarius YKJ-13T and that this cluster joined the clade comprising the type strains of two Marinibacillus species. Strain ASL-1T exhibited 16S rRNA gene sequence similarity values of 97.3 % to J. alimentarius YKJ-13T and 96.5 % to the type strains of Marinibacillus marinus and Marinibacillus campisalis. The chemotaxonomic properties of strain ASL-1T were similar to those of one or two of the genera Jeotgalibacillus and Marinibacillus. The peptidoglycan type was A1α linked directly through l-lysine as the diamino acid. Strain ASL-1T contained MK-7 as the predominant menaquinone with the presence of a significant amount of MK-8. The predominant fatty acid was anteiso-C15 : 0. The DNA G+C content was 42.9 mol%. Differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that strain ASL-1T could be differentiated from J. alimentarius and the two Marinibacillus species. On the basis of the data presented, strain ASL-1T represents a novel species within the genus Jeotgalibacillus, for which the name Jeotgalibacillus salarius sp. nov. is proposed. The type strain is ASL-1T (=KCTC 13257T=CCUG 56751T). It is also proposed that Marinibacillus marinus and Marinibacillus campisalis be reclassified as Jeotgalibacillus marinus comb. nov. (type strain 581T=DSM 1297T=ATCC 29841T=CCUG 28884T=CIP 103308T=LMG 6930T) and Jeotgalibacillus campisalis comb. nov. (type strain SF-57T=KCCM 41644T=JCM 11810T), respectively.

Virgibacillus byunsanensis sp. nov., isolated from a marine solar saltern

2010 ◽  
Vol 60 (2) ◽  
pp. 291-295 ◽  
Author(s):  
Jung-Hoon Yoon ◽  
So-Jung Kang ◽  
Yong-Taek Jung ◽  
Keun Chul Lee ◽  
Hyun Woo Oh ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Gene Sequence ◽  
Sequence Similarity ◽  
The Yellow Sea ◽  
16S Rrna Gene Sequence ◽  
Rrna Gene ◽  
Solar Saltern ◽  
Rrna Gene Sequence ◽  
Marine Solar Saltern

A Gram-variable, motile, endospore-forming and rod-shaped bacterial strain, ISL-24T, was isolated from a marine solar saltern of the Yellow Sea, Korea, and its taxonomic position was investigated by a polyphasic study. Strain ISL-24T grew optimally at pH 7.0–8.0, at 30–37 °C and in the presence of 8 % (w/v) NaCl. It contained MK-7 as the predominant menaquinone and anteiso-C15 : 0 as the predominant fatty acid. The DNA G+C content was 37.6 mol%. A phylogenetic analysis based on 16S rRNA gene sequences showed that strain ISL-24T fell within the genus Virgibacillus, clustering with Virgibacillus carmonensis LMG 20964T and Virgibacillus necropolis LMG 19488T, with a bootstrap resampling value of 92.3 %, and exhibiting 97.3 and 97.4 % 16S rRNA gene sequence similarity, respectively, to these strains. Strain ISL-24T exhibited 94.8–96.8 % 16S rRNA gene sequence similarity to the type strains of the other Virgibacillus species. Mean DNA–DNA relatedness values between strain ISL-24T and V. carmonensis DSM 14868T and V. necropolis DSM 14866T were 11 and 19 %, respectively. Differential phenotypic properties of strain ISL-24T, together with the phylogenetic and genetic distinctiveness, revealed that this strain is different from recognized Virgibacillus species. On the basis of phenotypic, phylogenetic and genetic data, strain ISL-24T represents a novel species of the genus Virgibacillus, for which the name Virgibacillus byunsanensis sp. nov. is proposed. The type strain is ISL-24T (=KCTC 13259T =CCUG 56754T).

scholarly journals Halobacillus yeomjeoni sp. nov., isolated from a marine solar saltern in Korea

2005 ◽  
Vol 55 (6) ◽  
pp. 2413-2417 ◽  
Author(s):  
Jung-Hoon Yoon ◽  
So-Jung Kang ◽  
Choong-Hwan Lee ◽  
Hyun Woo Oh ◽  
Tae-Kwang Oh
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Sequence Similarity ◽  
Phylogenetic Analyses ◽  
Rrna Gene ◽  
Solar Saltern ◽  
Marine Solar Saltern ◽  
Phenotypic Properties ◽  
Evolutionary Radiation ◽  
Type Strains

A Gram-positive or Gram-variable, motile, endospore-forming, halophilic bacterial strain, MSS-402T, was isolated from a marine solar saltern in Korea, and subjected to a polyphasic taxonomic study. Some cells of strain MSS-402T were long filamentous rods. The isolate grew optimally at 37 °C and in the presence of 3–5 % (w/v) NaCl. Strain MSS-402T had cell-wall peptidoglycan based on l-orn–d-Asp, MK-7 as the predominant menaquinone and anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0 as major fatty acids. The DNA G+C content was 42·9 mol%. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain MSS-402T falls within the evolutionary radiation of species of the genus Halobacillus. Levels of 16S rRNA gene sequence similarity between strain MSS-402T and the type strains of recognized Halobacillus species ranged from 98·0 % (with Halobacillus halophilus) to 99·2 % (with Halobacillus litoralis and Halobacillus trueperi). Levels of DNA–DNA binding indicated that strain MSS-402T represents a genomic species that is distinct from recognized Halobacillus species. Strain MSS-402T was differentiated from Halobacillus species by means of several phenotypic characteristics. On the basis of its phenotypic properties and phylogenetic and genetic distinctiveness, strain MSS-402T (=KCTC 3957T=DSM 17110T) should be classified as the type strain of a novel Halobacillus species, for which the name Halobacillus yeomjeoni sp. nov. is proposed.

scholarly journals Halobacillus campisalis sp. nov., containing meso-diaminopimelic acid in the cell-wall peptidoglycan, and emended description of the genus Halobacillus

2007 ◽  
Vol 57 (9) ◽  
pp. 2021-2025 ◽  
Author(s):  
Jung-Hoon Yoon ◽  
So-Jung Kang ◽  
Yong-Taek Jung ◽  
Tae-Kwang Oh
Keyword(s):  
Cell Wall ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
Sequence Similarity ◽  
Diaminopimelic Acid ◽  
The Yellow Sea ◽  
Rrna Gene ◽  
Phenotypic Properties ◽  
Cell Wall Peptidoglycan ◽  
Type Strains

A Gram-positive or variable, motile and coccoid or oval-shaped bacterial strain, ASL-17T, was isolated from a marine solar saltern of the Yellow Sea in Korea and its exact taxonomic position was investigated by a polyphasic approach. Strain ASL-17T grew optimally at pH 7.0–8.0 and 37 °C. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain ASL-17T is most closely affiliated phylogenetically to the genus Halobacillus. Strain ASL-17T exhibited 16S rRNA gene sequence similarity values of 97.7–98.6 % to the type strains of recognized Halobacillus species. Interestingly, strain ASL-17T had cell-wall peptidoglycan based on meso-diaminopimelic acid, unlike other Halobacillus species. It contained MK-7 as the predominant menaquinone. The major fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0. The DNA G+C content was 42.1 mol%. DNA–DNA relatedness data and differential phenotypic properties demonstrated that strain ASL-17T can be differentiated from recognized Halobacillus species. On the basis of phenotypic, chemotaxonomic, phylogenetic and genetic data, strain ASL-17T represents a novel species of the genus Halobacillus, for which the name Halobacillus campisalis sp. nov. is proposed. The type strain is ASL-17T (=KCTC 13144T =CCUG 54360T).

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