scholarly journals Characterization of the Antibiotic Compound No. 70 Produced byStreptomycessp. IMV-70

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Lyudmila P. Trenozhnikova ◽  
Almagul K. Khasenova ◽  
Assya S. Balgimbaeva ◽  
Galina B. Fedorova ◽  
Genrikh S. Katrukha ◽  
...  
Keyword(s):  
Optimal Growth ◽  
Growth Conditions ◽  
Culture Broth ◽  
Producer Strain ◽  
New Class ◽  
New Antibiotics ◽  
Actinomycete Strain ◽  
Main Component

We describe the actinomycete strain IMV-70 isolated from the soils of Kazakhstan, which produces potent antibiotics with high levels of antibacterial activity. After the research of its morphological, chemotaxonomic, and cultural characteristics, the strain with potential to be developed further as a novel class of antibiotics with chemotherapeutics potential was identified asStreptomycessp. IMV-70. In the process of fermentation, the strainStreptomycesspp. IMV-70 produces the antibiotic no. 70, which was isolated from the culture broth by extraction with organic solvents. Antibiotic compound no. 70 was purified and separated into individual components by HPLC, TLC, and column chromatography methods. The main component of the compound is the antibiotic 70-A, which was found to be identical to the peptolide etamycin A. Two other antibiotics 70-B and 70-C have never been described and therefore are new antibiotics. The physical-chemical and biological characteristics of these preparations were described and further researched. Determination of the optimal growth conditions to cultivate actinomycete-producer strain IMV-70 and development of methods to isolate, purify, and accumulate preparations of the new antibiotic no. 70 enable us to research further the potential of this new class of antibiotics.

Characterization of a hydrocarbon degrading psychrotrophic Antarctic bacterium

1997 ◽  
Vol 9 (2) ◽  
pp. 150-155 ◽  
Author(s):  
Walter P. Mac Cormack ◽  
Elda R. Fraile
Keyword(s):  
Culture Media ◽  
Growth Conditions ◽  
Culture Broth ◽  
Cold Climate ◽  
South Shetland Islands ◽  
Psychrotrophic Bacteria ◽  
Degrading Bacteria ◽  
Nacl Concentration ◽  
Initial Ph

Antarctic soil chronically exposed to gas-oil was analysed in order to isolate and study the growth conditions of hydrocarbon degrading bacteria. Soil samples taken near the shoreline in Jubany Station (King George Island, South Shetland Islands) were used as inoculum in liquid culture media with crude oil as sole carbon source. A psychrotrophic Acinetobacter strain was isolated and selected for further investigations. Effects were studied of temperature, initial pH, NaCl concentration and different chemical structure of the hydrocarbon on growth. Degradation rate was determined with n-dodecane and n-hexadecane. Growth of Acinetobacter ADH-1 showed no differences at an initial pH of 7.0, 7.5 and 8.0. Optimum temperature ranged between 25–30°C but the strain was capable of growing on n-dodecane at 4°C. Growth was observed in the presence of 3.5% NaCl. A decrease in the surface tension values was observed in the culture broth during the first 20 h of incubation (from 68 din cm−1 to 31 din cm−1). This proved to be related to the cellular fraction of the culture. The study shows that Acinetobacter ADH-1 is a psychrotrophic bacteria able to grow with hydrocarbons as sole carbon and energy source and could be potentially useful to design bioremediation processes in temperate and cold climate areas.

scholarly journals Catalytic Activity of Zr4+/ZA on The Isomerization Reaction of α-Pinene and It’s Reusability Test

2016 ◽  
Vol 1 (3) ◽  
pp. 157
Author(s):  
Nico Aditya Wijaya ◽  
Nanik Wijayati ◽  
Edy Cahyono
Keyword(s):  
Natural Zeolite ◽  
Raw Materials ◽  
Batch Reactor ◽  
Gravimetric Method ◽  
Isomerization Reaction ◽  
X Ray ◽  
Bet Method ◽  
Main Component

Production of turpentine oil from pine resin until December 2014<sup>th</sup> was reported to reaching 17.150 tones with the wide of pine forest is 876.992,66 acres. The main component of turpentine oil is α-pinena which isomer products have high value for industrial raw materials. Effects concentration of impregnation and reusability test a best catalyst on α-pinene isomerization reaction using catalysts Zr<sup>4+</sup>/natural zeolite in which modificated with different Zr<sup>4+</sup> concentration were analyzed. Characterization of catalyst included <em>X-ray Fluoroscent </em>for detection of Zr concentration in natural zeolites, <em>Surface Area Analyzer </em> for determine catalyst porosity using BET method, and determination of acidity using vapor ammonia and pyridine base adsorption gravimetric method. Isomerization process used a batch reactor with variation in the concentrations of Zr<sup>4+</sup> impregnations 10, 15, and 20 w/w as well as reusability test used best catalyst. The highest conversion of α-pinene  used Zr<sup>4+</sup>/ZA 10% catalyst was 3,89% so as Zr<sup>4+</sup>/ZA 10% catalyst is best catalyst than other catalysts whereas highest conversion of α-pinene used Zr<sup>4+</sup>/ZA 10% regeneration catalyst was 4,26%. The product is produced from isomerization reaction of α-pinene used Zr<sup>4+</sup>/ZA all variations catalysts was camphene and limonene.

scholarly journals Isolation and Characterization of Vibrio tubiashii Outer Membrane Proteins and Determination of a toxR Homolog

2007 ◽  
Vol 74 (3) ◽  
pp. 907-911 ◽  
Author(s):  
J. Jean-Gilles Beaubrun ◽  
M. H. Kothary ◽  
S. K. Curtis ◽  
N. C. Flores ◽  
B. E. Eribo ◽  
...  
Keyword(s):  
Membrane Proteins ◽  
Outer Membrane ◽  
Outer Membrane Proteins ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Growth Conditions ◽  
Isolation And Characterization ◽  
Vibrio Tubiashii

ABSTRACT Outer membrane proteins (OMPs) expressed by Vibrio tubiashii under different environmental growth conditions were characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, N-terminal amino acid sequencing, and PCR analyses. Results showed the presence of a 38- to 40-kDa OmpU-like protein and ompU gene, a maltoporin-like protein, several novel OMPs, and a regulatory toxR homolog.

scholarly journals Evaluation of the GPR (1.2 GHz) technique in the characterization of masonry shells of the Theatro Municipal do Rio de Janeiro

2020 ◽  
Vol 13 (2) ◽  
pp. 274-297
Author(s):  
D. C. B. CINTRA ◽  
P. M. B. MANHÃES ◽  
F. M. C. P. FERNANDES ◽  
D. M. ROEHL ◽  
J. T. ARARUNA JÚNIOR ◽  
...  
Keyword(s):  
Rio De Janeiro ◽  
Structural Integrity ◽  
Cultural Value ◽  
Sample Extraction ◽  
Characterization Of Materials ◽  
Main Component ◽  
Ground Penetrating ◽  
Non Destructive

Abstract The geometric characterization and evaluation of structural integrity are challenges for the preservation of historical buildings. The challenges are associated to the lack of constructive records, material diversity, access and contact restrictions, and sample extraction. Non-destructive tests are indicated in these cases. The objective of this paper is to evaluate the Ground Penetrating Radar (GPR) technique with 1.2 GHz antenna in the study of the determination of material thickness and discontinuities in the masonry shells of the Theatro Municipal do Rio de Janeiro, a heritage built in early 20th century, with high historical and cultural value. With paintings by renowned artists on their intrados, the domes and vault of brick masonry were at risk of collapse in the 1970s due to differential settlements in the supports and moisture, compromising their paintings. A strengthening intervention was carried out in 1976. Considering the masonry of bricks the main component, the electromagnetic wave propagation velocity was determined as 0,15 m/ns in one of the support walls, allowing to estimate the thicknesses of the elements ranging between 20 and 31 cm. Materials incorporated into the structure were identified: reinforcement, metal inserts and bricks. However, the frequency of the antenna did not allow the detection of damages. The application of the GPR to the characterization of materials and investigation of the integrity of the domes of the Theatro Municipal highlights the potential of this technology to study heritage and other constructions.

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