scholarly journals Crosstalk between chloroplast protein import and the SUMO system revealed through genetic and molecular investigation

2020 ◽  
Author(s):  
Samuel Watson ◽  
Na Li ◽  
Feijie Wu ◽  
Qihua Ling ◽  
R. Paul Jarvis
Keyword(s):  
Protein Import ◽  
Chloroplast Development ◽  
Nuclear Genome ◽  
Chloroplast Protein Import ◽  
Chloroplast Protein ◽  
Sumo Conjugation ◽  
Molecular Investigation ◽  
Regulatory Link ◽  
The Stability ◽  
Chloroplast Proteome

AbstractThe chloroplast proteome contains thousands of different proteins that are encoded by the nuclear genome. These proteins are imported into the chloroplast via the action of the TOC translocase and associated downstream systems. Our recent work has revealed that the stability of the TOC complex is dynamically regulated via the ubiquitin-dependent chloroplast-associated protein degradation (CHLORAD) pathway. Here, we demonstrate that the stability of the TOC complex is also regulated by the SUMO system. Arabidopsis mutants representing almost the entire SUMO conjugation pathway can partially suppress the phenotype of ppi1, a pale yellow mutant lacking the Toc33 protein. This suppression is linked to increased stability of TOC proteins and enhanced chloroplast development. In addition, we demonstrate using molecular and biochemical experiments that the SUMO system directly targets TOC proteins. Thus, we have identified a regulatory link between the SUMO system and chloroplast protein import.

scholarly journals Crosstalk between the chloroplast protein import and SUMO systems revealed through genetic and molecular investigation in Arabidopsis

eLife ◽  
2021 ◽  
Vol 10 ◽  
Author(s):  
Samuel Watson ◽  
Na Li ◽  
Yiting Ye ◽  
Feijie Wu ◽  
Qihua Ling ◽  
...  
Keyword(s):  
Protein Import ◽  
Chloroplast Development ◽  
Nuclear Genome ◽  
Chloroplast Protein Import ◽  
Chloroplast Protein ◽  
Sumo Conjugation ◽  
Molecular Investigation ◽  
Regulatory Link ◽  
The Stability ◽  
Chloroplast Proteome

The chloroplast proteome contains thousands of different proteins that are encoded by the nuclear genome. These proteins are imported into the chloroplast via the action of the TOC translocase and associated downstream systems. Our recent work has revealed that the stability of the TOC complex is dynamically regulated by the ubiquitin-dependent chloroplast-associated protein degradation (CHLORAD) pathway. Here, we demonstrate that the TOC complex is also regulated by the SUMO system. Arabidopsis mutants representing almost the entire SUMO conjugation pathway can partially suppress the phenotype of ppi1, a pale-yellow mutant lacking the Toc33 protein. This suppression is linked to increased abundance of TOC proteins and improvements in chloroplast development. Moreover, data from molecular and biochemical experiments support a model in which the SUMO system directly regulates TOC protein stability. Thus, we have identified a regulatory link between the SUMO system and the chloroplast protein import machinery.

scholarly journals Further analysis of the involvement of the envelope anion channel PIRAC in chloroplast protein import

2000 ◽  
Vol 267 (12) ◽  
pp. 3812-3817 ◽  
Author(s):  
Paul W. J. van den Wijngaard ◽  
Jeroen A. A. Demmers ◽  
Simon J. Thompson ◽  
Hans L. J. Wienk ◽  
Ben de Kruijff ◽  
...  
Keyword(s):  
Protein Import ◽  
Anion Channel ◽  
Chloroplast Protein Import ◽  
Chloroplast Protein

scholarly journals AtToc90, a New GTP-Binding Component of the Arabidopsis Chloroplast Protein Import Machinery

2004 ◽  
Vol 54 (3) ◽  
pp. 427-440 ◽  
Author(s):  
Andreas Hiltbrunner ◽  
Kathrin Grünig ◽  
Mayte Alvarez-Huerta ◽  
Sibylle Infanger ◽  
Jörg Bauer ◽  
...  
Keyword(s):  
Protein Import ◽  
Chloroplast Protein Import ◽  
Chloroplast Protein ◽  
Gtp Binding ◽  
Binding Component
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