scholarly journals Phenotypic and genotypic characterization of antibiotic Resistant Gram Negative Bacteria isolated in Tabuk City, Saudi Arabia

2021 ◽  
Author(s):  
Tarig M.S. Alnour ◽  
Elmutuz H. Elssaig ◽  
Eltayib H. Ahmed-Abakur ◽  
Faisel M. Abuduhier ◽  
Khalid A. S. Alfifi ◽  
...  
Keyword(s):  
Saudi Arabia ◽  
Dominant Gene ◽  
Cross Sectional Study ◽  
Automation System ◽  
Gram Negative Bacteria ◽  
Cross Sectional ◽  
Antibiotic Resistant ◽  
Gram Negative ◽  
Antimicrobial Surveillance ◽  
Current Therapies

AbstractAntimicrobial surveillance and identifying the genetic basis of antimicrobial resistance provide important information to optimize patient care. The present study was analytical cross sectional study aimed to determine the prevalence of MDR, XDR, PDR and extended-spectrum β-lactamases genes (SHV, CTX-M and TEM) among Gram-negative bacteria isolated in Tabuk, Saudi Arabia. A total number of 386 non-duplicate Gram-negative isolate were collected. Identification and susceptibility testing were done using automation system (BD Phoenix™). The extracted DNA were subjected to multiplex polymerase chain reaction (PCR). The results showed that only 15 (3.9%) of isolates were fully susceptible, the overall prevalence of XDR, MDR, PDR was 129 (33.4%), 113 (29.3%) and 48(12.4%) respectively. High resistant rate was observed against the antibiotic agents of cephalosporins class 79.3% followed by the agents of penicillins class 69.4%. The most dominant gene was bla SHV which detected in 106/386 (27.5%) isolates followed by bla CTX-M 90/386 (23.3%). Bla CTX-M showed significant relation with all used antibiotic except ampicillin/clavulanic acid, aztreonam, cefoxtin, and meropene. The isolates which showed most frequent resistant genes were Klebsiella pneumoniae 90/124 (72.6%), A. baumanni 37/67 (55.2%), and P.mirabilis 24/44 (54.5%). These findings underscores the need for optimization of current therapies and prevention of the spread of these organisms.

scholarly journals Cystic fibrosis pathogens survive for extended periods within cough-generated droplet nuclei

Thorax ◽  
2018 ◽  
Vol 74 (1) ◽  
pp. 87-90 ◽  
Author(s):  
Michelle E Wood ◽  
Rebecca E Stockwell ◽  
Graham R Johnson ◽  
Kay A Ramsay ◽  
Laura J Sherrard ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Cross Sectional Study ◽  
Gram Negative Bacteria ◽  
Sectional Study ◽  
Bacterial Strains ◽  
Cross Sectional ◽  
Gram Negative ◽  
Droplet Nuclei

The airborne route is a potential pathway in the person-to-person transmission of bacterial strains among cystic fibrosis (CF) populations. In this cross-sectional study, we investigate the physical properties and survival of common non-Pseudomonas aeruginosa CF pathogens generated during coughing. We conclude that Gram-negative bacteria and Staphylococcus aureus are aerosolised during coughing, can travel up to 4 m and remain viable within droplet nuclei for up to 45 min. These results suggest that airborne person-to-person transmission is plausible for the CF pathogens we measured.

scholarly journals Carbapenem-resistant Gram-negative bacteria isolated from poultry samples: a cross-sectional study

The Lancet ◽  
2019 ◽  
Vol 393 ◽  
pp. S21
Author(s):  
Abdelraouf A Elmanama ◽  
Mariam R Al-Reefi ◽  
Madleen A Shamali ◽  
Haya I Hemaid
Keyword(s):  
Cross Sectional Study ◽  
Gram Negative Bacteria ◽  
Sectional Study ◽  
Cross Sectional ◽  
Gram Negative ◽  
Carbapenem Resistant

Association between level of care and colonization with resistant gram-negative bacteria among nursing-home residents

2021 ◽  
pp. 1-3
Author(s):  
Timileyin Y Adediran ◽  
Kinjal N Sethuraman ◽  
Surbhi Leekha ◽  
Mary-Claire Roghmann
Keyword(s):  
Nursing Home ◽  
Odds Ratio ◽  
Nursing Home Residents ◽  
Cross Sectional Study ◽  
Level Of Care ◽  
Gram Negative Bacteria ◽  
Sectional Study ◽  
Cross Sectional ◽  
Gram Negative ◽  
The Relationship

Abstract In this cross-sectional study, we examined the relationship between resident level of care in the nursing home and colonization with resistant gram-negative bacteria. Residential-care residents were more likely to be colonized with resistant gram-negative bacteria than were postacute care residents (odds ratio, 2.3; 95% confidence interval, 1.40–3.80; P < .001).

scholarly journals Gram Negative Bacteria Associated with Spoilage of Tomatoes Sold at Mararaba and Masaka Market, New Karu Local Government Area, Nasarawa State

2019 ◽  
pp. 1-7
Author(s):  
A. L. Olokun ◽  
A. B. Ajide ◽  
J. O. Ihuma
Keyword(s):  
Local Government ◽  
Pathogenic Bacteria ◽  
Cross Sectional Study ◽  
Local Government Area ◽  
Biochemical Test ◽  
Gram Negative Bacteria ◽  
Salmonella Spp ◽  
Cross Sectional ◽  
Gram Negative ◽  
Esherichia Coli

Aims: The study is aimed at isolating and identifying the gram negative bacteria associated with spoilage of tomatoes at Mararaba and Masaka markets, New Karu Local Government Area, Nasarawa State. Study Design: A cross-sectional study to isolate gram negative bacteria associated with spoilage of tomatoes at Mararaba and Masaka markets, New Karu Local Government Area, Nasarawa State. Place and Duration of Study: The isolation of gram negative bacteria was carried out at the Department of Biological Sciences, Bingham University New Karu, Nasarawa State, Nigeria, between January, 2018 and September, 2018. Methodology: The samples collected were crushed with a sterile mortar and pestle after which tenfold serial dilution was carried out using sterile distilled water before inoculating on MaCconkey and Blood agar using the pour plate technique. Colonies were then subjected to gram staining and biochemical test. Results: From the result of the biochemical test, Salmonella spp, Esherichia coli spp, Klebsiella spp and vibrio spp. were the gram-negative bacteria isolated from the tomato samples collected at both markets. Conclusion: Tomatoes sold in an unclean environment that is contamination with food- borne pathogenic bacteria poses a risk to the consumers.

scholarly journals Anti-microbial resistant in Gram-negative bacteria bacillus: Enterobacteriaceae and non-fermenting bacilli isolated at Sominé DOLO Hospital of Mopti, Mali

2022 ◽  
Vol 18 (1) ◽  
pp. 008-013
Author(s):  
Moussa Diawara ◽  
Modibo Coulibaly ◽  
Dramane Samaké ◽  
Soumaila Touré ◽  
Dramane Cissé ◽  
...  
Keyword(s):  
Hospital Setting ◽  
Cross Sectional Study ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Sensitivity Testing ◽  
Cross Sectional ◽  
Gram Negative ◽  
Epidemiological Aspect ◽  
Antibiotics Sensitivity ◽  
High Level

Background: β-lactams and carbapenems. are the major antibiotics used to treat gram-negative bacteria and non-fermenting bacilli. However, the increasing production of β-lactamase and carbapenemase limits the therapeutic options. Our study aims to determine the resistant phenotypes of these bacteria while describing their epidemiological aspect. Material and Methods: This was a cross-sectional study by consecutive enrollment from January 2018 to December 2019 at Sominé DOLO Hospital of Mopti, Mali. We performed manual method for bacteria culture, identification and antibiotics sensitivity testing. The antibiotics sensitivity testing was accessed by the diffusion method according to CA-SFM/EUCAST (“Comité de l’Antibiogramme de la Société Française de Microbiologie” / European Committee on Antimicrobial Susceptibility Testing) recommendations V1.0 february 2018 and V2.0 may 2019 2019 V.2.0. May recommendation released in 2019. Data were analyzed by software R 4.0.3 GUI 1.73 Catalina build (7892). Results: At all 904 samples were included in this study. Out of the 904 cultures, 297 sample (32.85%) were positive. The rates of enzymes production were as follow: Extended-spectrum ß-lactamase (ESBL) 56.42% (101/179), cepholosporinase hyperproduction (HCASE) 15.64% (28/179), cephalosporinase production (CASE) 6.14% (11/179), penicillinase hyperproduction (HP) 5.58% (10/179), carbapenemase production (CP) 6.14% (11/179) and savage strains 10.05% (18/179). Conclusion: Our data showed a high prevalence of resistance to β-lactamins ß-talactamins and carbapenemes in gram-negative bacteria and non-fermenting bacillus bacilli. The A high level of β-lactamase and carbapenemase production by gram negative bacillus were also reported by others authors calls for the rational use of antibiotic in hospital setting.

scholarly journals Anti-microbial resistant in Gram-negative bacteria bacillus: Enterobacteriaceae and non-fermenting bacilli isolated at Sominé DOLO Hospital of Mopti, Mali

2022 ◽  
Vol 18 (1) ◽  
pp. 008-013
Author(s):  
Moussa Diawara ◽  
Modibo Coulibaly ◽  
Dramane Samaké ◽  
Soumaila Touré ◽  
Dramane Cissé ◽  
...  
Keyword(s):  
Hospital Setting ◽  
Cross Sectional Study ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Sensitivity Testing ◽  
Cross Sectional ◽  
Gram Negative ◽  
Epidemiological Aspect ◽  
Antibiotics Sensitivity ◽  
High Level

Background: β-lactams and carbapenems. are the major antibiotics used to treat gram-negative bacteria and non-fermenting bacilli. However, the increasing production of β-lactamase and carbapenemase limits the therapeutic options. Our study aims to determine the resistant phenotypes of these bacteria while describing their epidemiological aspect. Material and Methods: This was a cross-sectional study by consecutive enrollment from January 2018 to December 2019 at Sominé DOLO Hospital of Mopti, Mali. We performed manual method for bacteria culture, identification and antibiotics sensitivity testing. The antibiotics sensitivity testing was accessed by the diffusion method according to CA-SFM/EUCAST (“Comité de l’Antibiogramme de la Société Française de Microbiologie” / European Committee on Antimicrobial Susceptibility Testing) recommendations V1.0 february 2018 and V2.0 may 2019 2019 V.2.0. May recommendation released in 2019. Data were analyzed by software R 4.0.3 GUI 1.73 Catalina build (7892). Results: At all 904 samples were included in this study. Out of the 904 cultures, 297 sample (32.85%) were positive. The rates of enzymes production were as follow: Extended-spectrum ß-lactamase (ESBL) 56.42% (101/179), cepholosporinase hyperproduction (HCASE) 15.64% (28/179), cephalosporinase production (CASE) 6.14% (11/179), penicillinase hyperproduction (HP) 5.58% (10/179), carbapenemase production (CP) 6.14% (11/179) and savage strains 10.05% (18/179). Conclusion: Our data showed a high prevalence of resistance to β-lactamins ß-talactamins and carbapenemes in gram-negative bacteria and non-fermenting bacillus bacilli. The A high level of β-lactamase and carbapenemase production by gram negative bacillus were also reported by others authors calls for the rational use of antibiotic in hospital setting.

scholarly journals Bacteremia in Diarrheal Children With Severe Pneumonia

2019 ◽  
Vol 6 ◽  
pp. 2333794X1986246
Author(s):  
Haimanti Saha ◽  
Lubaba Shahrin ◽  
Monira Sarmin ◽  
Tahmeed Ahmed ◽  
Mohammod Jobayer Chisti
Keyword(s):  
Diarrheal Disease ◽  
Severe Pneumonia ◽  
Cross Sectional Study ◽  
Childhood Mortality ◽  
Gram Negative Bacteria ◽  
Limited Information ◽  
Bacterial Isolates ◽  
Cross Sectional ◽  
Gram Negative ◽  
The Difference

Objectives. Diarrhea and pneumonia are the leading causes of under-5 childhood mortality. However, there is limited information on bacterial etiology of severe pneumonia in children with diarrhea. We analyzed bacterial pathogens from the blood of children under the age of 5 years. Methods. In this retrospective cross-sectional study, we studied all children having severe pneumonia with or without diarrhea admitted to the icddr,b (International Centre for Diarrheal Disease Research, Bangladesh) who had their blood culture done during January 2014 to December 2014. Results. Among a total of 159 study children, 118 had diarrhea. There were 13 bacterial isolates, and predominant organisms were gram-negative bacteria (11/13, 85%). Children with diarrhea coexisting with severe pneumonia proportionately had higher bacteremia (12/141 [10.16%] vs 1/41 [2.43%]), but the difference was not statistically insignificant ( P = .186). Conclusion. We recognized that the coexistence of diarrhea and severe pneumonia had proportionately higher bacteremia, especially gram-negative bacteria compared with those without diarrhea. The results emphasize the trend of bacterial etiology of pneumonia in children with diarrhea and may warrant revised antibiotics guideline for their management.

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