scholarly journals Sequence data from the internal and external transcribed spacers of nuclear ribosomal DNA of Cyclamen purpurascens allow geographic mapping

2018 ◽  
Author(s):  
Hannah Miriam Jaag

AbstractCyclamen purpurascens (Alpine, European or purple cyclamen) is native to central Europe. Since decades it is discussed wether the occurrences of C. purpurascens north of the alps is native or if it was introduced. Here the nuclear ribosomal DNAs (rDNA) are sequenced in oder to obtain a phylogenetic geographic pattern. Phylogenetic analyses of ITS and NTS/ETS sequences distinguish three main clades coinciding with geographical distribution: Eastern alps (Austria), southern alps (Switzerland, Italy) and western Alps (France). The paper presents interspecific relationship of C. purpurascens based on geographic sequences of rDNA. The observed variations suggest that some plants were introduced via Benedictine gardens and the plants from Monastery gardens seem to origin from Lower Austria.

Mammalia ◽  
2019 ◽  
Vol 83 (2) ◽  
pp. 180-189 ◽  
Author(s):  
Adam W. Ferguson ◽  
Houssein R. Roble ◽  
Molly M. McDonough

AbstractThe molecular phylogeny of extant genets (Carnivora, Viverridae,Genetta) was generated using all species with the exception of the Ethiopian genetGenetta abyssinica. Herein, we provide the first molecular phylogenetic assessment ofG. abyssinicausing molecular sequence data from multiple mitochondrial genes generated from a recent record of this species from the Forêt du Day (the Day Forest) in Djibouti. This record represents the first verified museum specimen ofG. abyssinicacollected in over 60 years and the first specimen with a specific locality for the country of Djibouti. Multiple phylogenetic analyses revealed conflicting results as to the exact relationship ofG. abyssinicato otherGenettaspecies, providing statistical support for a sister relationship to all other extant genets for only a subset of mitochondrial analyses. Despite the inclusion of this species for the first time, phylogenetic relationships amongGenettaspecies remain unclear, with limited nodal support for many species. In addition to providing an alternative hypothesis of the phylogenetic relationships among extant genets, this recent record provides the first complete skeleton of this species to our knowledge and helps to shed light on the distribution and habitat use of this understudied African small carnivore.


Zootaxa ◽  
2012 ◽  
Vol 3401 (1) ◽  
pp. 49 ◽  
Author(s):  
SARP KAYA ◽  
DRAGAN CHOBANOV ◽  
BATTAL ÇIPLAK

The new species Anterastes davrazensis sp. n. (Orthoptera, Tettigoniidae) is described from south-eastern Turkey. Description, diagnosis and relationships of the new species were studied utilizing morphology, male calling songs and 16S rDNA sequence data from all species in the genus. Morphology and song syllable structure indicate A. davrazensis sp. n. is related to A. uludaghensis. Phylogenetic analyses based on representative haplotypes of 16S rDNA, using Sureyaella bella, Parapholidoptera distincta and Bolua turkiyae as outgroups, also suggested strong support to the relationship of these two species. A. davrazensis sp. n. differs from its closest relative A. uludaghensis by the higher number of stridulatory pegs and the song, consisting of irregular syllable groups.


2014 ◽  
Vol 62 (3) ◽  
pp. 235 ◽  
Author(s):  
S. Safaei Chaei Kar ◽  
F. Ghanavati ◽  
M. R. Naghavi ◽  
H. Amirabadi-zade ◽  
R. Rabiee

Onobrychis, comprising more than 130 species, is a genus of the family Fabaceae. At this time, the interspecies relationship of this biologically important genus is still a subject of great discussion and debate. To help resolve this disagreement, we used molecular phylogeny to analyse internal transcribed spacer (ITS) and trnL–trnF sequences of 76 species of Onobrychis. Bayesian interference, maximum parsimony and maximum likelihood analyses of nuclear ITS and plastid trnL–trnF DNA sequence data generated trees with strong posterior probability for two groups: Onobrychis subgen. Sisyrosema (including: Heliobrychis, Hymenobrychis, Afghanicae and Anthyllium sections) along with Laxiflorae section in Group I and Onobrychis subgen. Onobrychis (except Laxiflorae section) in the other (Group II). The Laxiflorae section roots back to the ancestral node for Sisyrosema subgen. O. viciifolia (cultivated species), which is closely associated with O. cyri var. cyri, suggesting that the latter may be a wild progenitor of O. viciifolia. The present study supported the paraphyly of subgenera Onobrychis and Sisyrosema. The study proposed the paraphyletic nature of the sections Onobrychis, Dendrobrychis, Heliobrychis and Hymenobrychis. Together with our molecular phylogenetic analyses we present a review of Onobrychis morphology and discuss and compare our results with those of earlier morphological and molecular phylogenetic analyses.


Pathogens ◽  
2020 ◽  
Vol 9 (12) ◽  
pp. 1042 ◽  
Author(s):  
Tanapan Sukee ◽  
Anson V. Koehler ◽  
Ross Hall ◽  
Ian Beveridge ◽  
Robin B. Gasser ◽  
...  

Nematodes of the genus Macropostrongyloides inhabit the large intestines or stomachs of macropodid (kangaroos and wallabies) and vombatid (wombats) marsupials. This study established the relationships of seven species of Macropostrongyloides using mitochondrial (mt) protein amino acid sequence data sets. Phylogenetic analyses revealed that species of Macropostrongyloides (M. lasiorhini, M. baylisi, M. yamagutii, M. spearei, M. mawsonae and M. woodi) from the large intestines of their hosts formed a monophyletic assemblage with strong nodal support to the exclusion of M. dissimilis from the stomach of the swamp wallaby. Furthermore, the mitochondrial protein-coding genes provided greater insights into the diversity and phylogeny of the genus Macropostrongyloides; such data sets could potentially be used to elucidate the relationships among other parasitic nematodes of Australian marsupials.


2006 ◽  
Vol 84 (2) ◽  
pp. 269-281 ◽  
Author(s):  
Suzanne I. Warwick ◽  
Ihsan A. Al-Shehbaz ◽  
Connie A. Sauder

Sequence data from the nuclear ribosomal internal transcribed spacer (ITS) region of 45 taxa were used to determine the phylogenetic relationship of Arabis arenicola to Arabis , Arabidopsis , Braya , and Eutrema , and that of Eutrema to the purportedly related genera Aphragmus , Lignariella , Neomartinella , Platycraspedum , Taphrospermum , and Thellungiella . Arabis arenicola was originally described as Eutrema in 1830, transferred to Arabis in 1898, and has remained in Arabis to the present, even though it is morphologically more similar to Arabidopsis, Braya, and Eutrema. Sequence data were obtained from representative taxa of Arabis, Arabidopsis, and related Boechera and Catolobus, Braya and Neotorularia, and Eutrema, Aphragmus, Lignariella, Neomartinella, Platycraspedum, Taphrospermum, and Thellungiella. The five Arabis arenicola accessions examined had ITS sequences that were identical to each other and to four Arabidopsis lyrata accessions. In both maximum parsimony and maximum likelihood analyses, Arabis arenicola fell within the Arabidopsis clade and was closely aligned with Arabidopsis lyrata. Two of six purportedly related genera were not closely related to Eutrema. Both analyses placed Lignariella within a separate well-supported clade with Aphragmus, while the other four genera, Neomartinella, Platycraspedum, Taphrospermum, and Thellungiella, fell within a well-supported clade with Eutrema. Morphology and molecular data strongly suggest transferring Arabis arenicola to Arabidopsis, expanding Aphragmus to include Lignariella, and expanding Eutrema to include Neomartinella, Platycraspedum, Taphrospermum, and Thellungiella. New combinations in Arabidopsis and Aphragmus are proposed.


2021 ◽  
Author(s):  
Scarlett Zetter ◽  
Sandra Garcés Pastor ◽  
Youri Lammers ◽  
Andreas Tribsch ◽  
Antony G. Brown ◽  
...  

<p>The Alps contain highly biodiverse ecosystems including a large number endemic flora. As a result of climate change and anthropogenic activities, such ecosystems are at risk from upward vegetation displacement and species loss. Extensive archaeological research in the Eastern Alps has documented human settlement from ~5500 years ago driven by salt and copper mining; which has caused significant impact on the ecosystems through mining, deforestation, and pastoral farming. To elucidate the effects of climate change and anthropogenic activities on plant biodiversity, multi-proxy reconstructions have been carried out throughout the Western Alps . Despite this research, the palaeoecological history of the Eastern Alps is relatively understudied. Consequently, we are limited in our understanding of how climate change and human impact have affected past biodiversity and the formation of the contemporary vegetation in this region. Here, we focus on the Austrian sub-alpine lake, Großer Winterleitensee located at the Easternmost margin of the Alps; only locally glaciated during the Pleistocene. We applied sedimentary ancient DNA (sedaDNA) metabarcoding to reconstruct Holocene plant community dynamics within the lake catchment. These data, in conjunction with local temperature reconstructions, sediment elemental composition, magnetic susceptibility, and loss-on-ignition analyses, allowed us to identify key intervals of plant diversity change. Two such intervals begin at samples dated ~5500 cal. yr BP and ~2200 cal. yr BP, coinciding with Neolithic and Iron Age settlement phases in the area. Palaeoecological reconstructions of plant biodiversity and their responses to climate change and anthropogenic pressures may be able to provide essential information for future conservation purposes.</p><div> <div> <div> </div> </div> <div> <div> </div> </div> <div> <div> </div> </div> </div>


Mycotaxon ◽  
2020 ◽  
Vol 135 (1) ◽  
pp. 143-150
Author(s):  
Xin Zhang ◽  
Kun-Ying Wang ◽  
Peng-Peng Ren ◽  
Yu-Lan Jiang

A new species, Ochroconis terricola, was isolated from soil in Guizhou Province, China. Morphology and phylogenetic analyses of the combined sequence data of nuclear ribosomal DNA genes (ITS, LSU, SSU) revealed the strain as different from other Ochroconis species.


Parasitology ◽  
2019 ◽  
Vol 146 (13) ◽  
pp. 1673-1682 ◽  
Author(s):  
Tanapan Sukee ◽  
Ian Beveridge ◽  
Neil B. Chilton ◽  
Abdul Jabbar

AbstractThe genetic variation and taxonomic status of the four morphologically-defined species of Macropostrongyloides in Australian macropodid and vombatid marsupials were examined using sequence data of the ITS+ region (=first and second internal transcribed spacers, and the 5.8S rRNA gene) of the nuclear ribosomal DNA. The results of the phylogenetic analyses revealed that Ma. baylisi was a species complex consisting of four genetically distinct groups, some of which are host-specific. In addition, Ma. lasiorhini in the common wombat (Vombatus ursinus) did not form a monophyletic clade with Ma. lasiorhini from the southern hairy-nosed wombat (Lasiorhinus latifrons), suggesting the possibility of cryptic (genetically distinct but morphologically similar) species. There was also some genetic divergence between Ma. dissimilis in swamp wallabies (Wallabia bicolor) from different geographical regions. In contrast, there was no genetic divergence among specimens of Ma. yamagutii across its broad geographical range or between host species (i.e. Macropus fuliginosus and M. giganteus). Macropostrongyloides dissimilis represented the sister taxon to Ma. baylisi, Ma. yamagutii and Ma. lasiorhini. Further morphological and molecular studies are required to assess the species complex of Ma. baylisi.


Diversity ◽  
2021 ◽  
Vol 13 (11) ◽  
pp. 516
Author(s):  
Xian-Dong Yu ◽  
Sheng-Nan Zhang ◽  
Ratchadawan Cheewangkoon ◽  
Jian-Kui Liu

During a survey of microfungi associated with palms from karst formations, three novel ascomycetes were found from decaying petioles of Trachycarpus fortunei (Arecaceae) in Guizhou, China. Multi-gene phylogenetic analyses based on a combined SSU, ITS, LSU, RPB2 and TEF1α sequence data showed that these collections were affiliated to Brunneofusispora and Neooccultibambusa in the family Occultibambusaceae. A new species Brunneofusispora inclinatiostiola is introduced. It is phylogenetically close to B. clematidis but represents a distinct lineage. Morphologically, it differs from the latter in having immersed ascomata with eccentric, periphysate ostiole and smaller ascospores. Morpho-phylogenetic evidence also revealed two new Neooccultibambusa species, N. kaiyangensis and N. trachycarpi. Together with the generic type N. chiangraiensis, they formed a distinct lineage within the genus Neooccultibambusa. Three novel palmicolous fungi of Occultibambusaceae are described, illustrated and notes on their identification are provided. The ecological significance of the new taxa and the phylogenetic relationship of genera in Occultibambusaceae is discussed.


2019 ◽  
Vol 94 ◽  
Author(s):  
I. Blasco-Costa ◽  
K. Seppälä ◽  
F. Feijen ◽  
N. Zajac ◽  
K. Klappert ◽  
...  

Abstract The adult and metacercaria life stages of a new species of the microphallid genus Atriophallophorus Deblock & Rosé, 1964 are described from specimens collected at Lake Alexandrina (South Island, New Zealand). In addition to molecular analyses of ribosomal and mitochondrial genes, metacercariae of Atriophallophorus winterbourni n. sp. from the snail host Potamopyrgus antipodarum (Gray) were grown in vitro to characterize internal and external morphology of adults using light and scanning electron microscopy and histological techniques. Atriophallophorus winterbourni n. sp. is readily distinguishable from Atriophallophorus coxiellae Smith, 1973 by having a different structure of the prostatic chamber, sub-circular and dorsal to genital atrium, rather than cylindrical, fibrous, elongate and placed between the seminal vesicle and the genital atrium. The new species is most similar to Atriophallophorus minutus (Price, 1934) with regards to the prostatic chamber and the morphometric data, but possesses elongate-oval testes and subtriangular ovary rather than oval and transversely oval in A. minutus. Phylogenetic analyses including sequence data for A. winterbourni n. sp. suggested a congeneric relationship of the new species to a hitherto undescribed metacercariae reported from Australia, both forming a strongly supported clade closely related to Microphallus and Levinseniella. In addition, we provide an amended diagnosis of Atriophallophorus to accommodate the new species and confirm the sinistral interruption of the outer rim of the ventral sucker caused by the protrusion of the dextral parietal atrial scale at the base of the phallus.


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