Real-Time Protein-Fragment Complementation Assays for Studying Temporal, Spatial, and Spatiotemporal Dynamics of Protein–Protein Interactions in Living Cells

Mohan Malleshaiah; Emmanuelle Tchekanda; Stephen W. Michnick

doi:10.1101/pdb.prot090068

Real-Time Protein-Fragment Complementation Assays for Studying Temporal, Spatial, and Spatiotemporal Dynamics of Protein–Protein Interactions in Living Cells

Cold Spring Harbor Protocols ◽

10.1101/pdb.prot090068 ◽

2016 ◽

Vol 2016 (11) ◽

pp. pdb.prot090068 ◽

Cited By ~ 2

Author(s):

Mohan Malleshaiah ◽

Emmanuelle Tchekanda ◽

Stephen W. Michnick

Keyword(s):

Real Time ◽

Protein Interactions ◽

Living Cells ◽

Spatiotemporal Dynamics ◽

Protein Protein Interactions ◽

Protein Fragment ◽

Protein Fragment Complementation ◽

Fragment Complementation

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Corrigendum: Real-Time Protein-Fragment Complementation Assays for Studying Temporal, Spatial, and Spatiotemporal Dynamics of Protein–Protein Interactions in Living Cells

Cold Spring Harbor Protocols ◽

10.1101/pdb.corr107815 ◽

2022 ◽

Vol 2022 (1) ◽

pp. pdb.corr107815

Author(s):

Mohan Malleshaiah ◽

Emmanuelle Tchekanda ◽

Stephen W. Michnick

Keyword(s):

Real Time ◽

Protein Interactions ◽

Living Cells ◽

Spatiotemporal Dynamics ◽

Protein Protein Interactions ◽

Protein Fragment ◽

Protein Fragment Complementation ◽

Fragment Complementation

Download Full-text

Extended Linkers Improve the Detection of Protein-protein Interactions (PPIs) by Dihydrofolate Reductase Protein-fragment Complementation Assay (DHFR PCA) in Living Cells

Molecular & Cellular Proteomics ◽

10.1074/mcp.tir117.000385 ◽

2017 ◽

Vol 17 (2) ◽

pp. 373-383 ◽

Cited By ~ 5

Author(s):

Andrée-Ève Chrétien ◽

Isabelle Gagnon-Arsenault ◽

Alexandre K. Dubé ◽

Xavier Barbeau ◽

Philippe C. Després ◽

...

Keyword(s):

Dihydrofolate Reductase ◽

Protein Interactions ◽

Living Cells ◽

Protein Protein Interactions ◽

Protein Fragment ◽

Complementation Assay ◽

Protein Fragment Complementation ◽

Fragment Complementation ◽

Protein Fragment Complementation Assay

Download Full-text

Extended linkers improve the detection of protein-protein interactions (PPIs) by dihydrofolate reductase protein-fragment complementation assay (DHFR PCA) in living cells

Molecular & Cellular Proteomics ◽

10.1074/mcp.a117.000385 ◽

2018 ◽

Vol 17 (3) ◽

pp. 549-549 ◽

Cited By ~ 5

Author(s):

Andrée-Ève Chrétien ◽

Isabelle Gagnon-Arsenault ◽

Alexandre K. Dubé ◽

Xavier Barbeau ◽

Philippe C. Després ◽

...

Keyword(s):

Dihydrofolate Reductase ◽

Protein Interactions ◽

Living Cells ◽

Protein Protein Interactions ◽

Protein Fragment ◽

Complementation Assay ◽

Protein Fragment Complementation ◽

Fragment Complementation ◽

Protein Fragment Complementation Assay

Download Full-text

Protein-Fragment Complementation Assays for Large-Scale Analysis, Functional Dissection and Dynamic Studies of Protein–Protein Interactions in Living Cells

Methods in Molecular Biology - Signal Transduction Protocols ◽

10.1007/978-1-61779-160-4_25 ◽

2011 ◽

pp. 395-425 ◽

Cited By ~ 20

Author(s):

Stephen W. Michnick ◽

Po Hien Ear ◽

Christian Landry ◽

Mohan K. Malleshaiah ◽

Vincent Messier

Keyword(s):

Protein Interactions ◽

Large Scale ◽

Living Cells ◽

Scale Analysis ◽

Protein Protein Interactions ◽

Protein Fragment ◽

Dynamic Studies ◽

Large Scale Analysis ◽

Protein Fragment Complementation ◽

Fragment Complementation

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A Toolkit of Protein-Fragment Complementation Assays for Studying and Dissecting Large-Scale and Dynamic Protein–Protein Interactions in Living Cells

Methods in Enzymology - Guide to Yeast Genetics: Functional Genomics, Proteomics, and Other Systems Analysis ◽

10.1016/s0076-6879(10)70014-8 ◽

2010 ◽

pp. 335-368 ◽

Cited By ~ 28

Author(s):

Stephen W. Michnick ◽

Po Hien Ear ◽

Christian Landry ◽

Mohan K. Malleshaiah ◽

Vincent Messier

Keyword(s):

Protein Interactions ◽

Large Scale ◽

Living Cells ◽

Protein Protein Interactions ◽

Protein Fragment ◽

Protein Fragment Complementation ◽

Fragment Complementation

Download Full-text

Extended Linkers Improve the Detection of Protein-protein Interactions (PPIs) by Dihydrofolate Reductase Protein-fragment Complementation Assay (DHFR PCA) in Living Cells

Molecular & Cellular Proteomics ◽

10.1074/mcp.ra117.000385 ◽

2018 ◽

Vol 17 (2) ◽

pp. 373-383

Author(s):

Andrée-Ève Chrétien ◽

Isabelle Gagnon-Arsenault ◽

Alexandre K. Dubé ◽

Xavier Barbeau ◽

Philippe C. Després ◽

...

Keyword(s):

Dihydrofolate Reductase ◽

Protein Interactions ◽

Living Cells ◽

Protein Protein Interactions ◽

Protein Fragment ◽

Complementation Assay ◽

Protein Fragment Complementation ◽

Fragment Complementation ◽

Protein Fragment Complementation Assay

Download Full-text

Optimizing Luciferase Protein Fragment Complementation for Bioluminescent Imaging of Protein–Protein Interactions in Live Cells and Animals

Methods in Enzymology - Imaging in Biological Research, Part A ◽

10.1016/s0076-6879(04)85019-5 ◽

2004 ◽

pp. 349-360 ◽

Cited By ~ 35

Author(s):

Kathryn E Luker ◽

David Piwnica-Worms

Keyword(s):

Protein Interactions ◽

Live Cells ◽

Bioluminescent Imaging ◽

Protein Protein Interactions ◽

Protein Fragment ◽

Protein Fragment Complementation ◽

Luciferase Protein ◽

Fragment Complementation

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Discovering Protein-Protein Interactions within the Programmed Cell Death Network Using a Protein-Fragment Complementation Screen

Cell Reports ◽

10.1016/j.celrep.2014.06.049 ◽

2014 ◽

Vol 8 (3) ◽

pp. 909-921 ◽

Cited By ~ 23

Author(s):

Yuval Gilad ◽

Ruth Shiloh ◽

Yaara Ber ◽

Shani Bialik ◽

Adi Kimchi

Keyword(s):

Cell Death ◽

Programmed Cell Death ◽

Protein Interactions ◽

Protein Protein Interactions ◽

Protein Fragment ◽

Protein Fragment Complementation ◽

Fragment Complementation

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Detection of Protein-Protein Interactions Using Protein-Fragment Complementation Assays (PCA)

Current Proteomics ◽

10.2174/157016407781387348 ◽

2007 ◽

Vol 4 (1) ◽

pp. 17-27 ◽

Cited By ~ 6

Author(s):

Emma Barnard ◽

Neil V. McFerran ◽

John Nelson ◽

David J. Timson

Keyword(s):

Protein Interactions ◽

Protein Protein Interactions ◽

Protein Fragment ◽

Protein Fragment Complementation ◽

Fragment Complementation

Download Full-text

Using the β-lactamase protein-fragment complementation assay to probe dynamic protein–protein interactions

Nature Protocols ◽

10.1038/nprot.2007.356 ◽

2007 ◽

Vol 2 (9) ◽

pp. 2302-2306 ◽

Cited By ~ 21

Author(s):

Ingrid Remy ◽

Galia Ghaddar ◽

Stephen W Michnick

Keyword(s):

Protein Interactions ◽

Protein Protein Interactions ◽

Protein Fragment ◽

Complementation Assay ◽

Protein Fragment Complementation ◽

Fragment Complementation ◽

Protein Fragment Complementation Assay

Download Full-text