scholarly journals Microarray Analysis of the Genome-Wide Response to Iron Deficiency and Iron Reconstitution in the Cyanobacterium Synechocystis sp. PCC 6803

2003 ◽  
Vol 132 (4) ◽  
pp. 1825-1839 ◽  
Author(s):  
Abhay K. Singh ◽  
Lauren M. McIntyre ◽  
Louis A. Sherman
Keyword(s):  
Iron Deficiency ◽  
Microarray Analysis ◽  
Genome Wide ◽  
Synechocystis Sp ◽  
Pcc 6803

scholarly journals Genome-wide microarray analysis of tomato roots showed defined responses to iron deficiency

BMC Genomics ◽  
2012 ◽  
Vol 13 (1) ◽  
pp. 101 ◽  
Author(s):  
Anita Zamboni ◽  
Laura Zanin ◽  
Nicola Tomasi ◽  
Mario Pezzotti ◽  
Roberto Pinton ◽  
...  
Keyword(s):  
Iron Deficiency ◽  
Microarray Analysis ◽  
Genome Wide ◽  
Tomato Roots

scholarly journals Serine/Threonine Protein Kinase SpkA in Synechocystis sp. Strain PCC 6803 Is a Regulator of Expression of Three Putative pilA Operons, Formation of Thick Pili, and Cell Motility

2006 ◽  
Vol 188 (21) ◽  
pp. 7696-7699 ◽  
Author(s):  
Vladimir B. Panichkin ◽  
Satoko Arakawa-Kobayashi ◽  
Toku Kanaseki ◽  
Iwane Suzuki ◽  
Dmitry A. Los ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Protein Kinase ◽  
Cell Motility ◽  
Microarray Analysis ◽  
Dna Microarray ◽  
Dna Microarray Analysis ◽  
Synechocystis Sp ◽  
Pcc 6803

ABSTRACT Previous studies showed that a Ser/Thr protein kinase, SpkA, in Synechocystis sp. strain PCC 6803 is involved in cell motility. The present study, in which DNA microarray analysis and electron microscopy were used, demonstrated that SpkA regulates the expression of putative pilA9-pilA10-pilA11-slr2018, pilA5-pilA6, and pilA1-pilA2 operons and is essential for the formation of thick pili.

scholarly journals Minor pilin genes are involved in motility and natural competence in Synechocystis sp. PCC 6803

2020 ◽  
Author(s):  
Sabrina Oeser ◽  
Thomas Wallner ◽  
Nils Schuergers ◽  
Annegret Wilde ◽  
Lenka Bucinska ◽  
...  
Keyword(s):  
Cell Surface ◽  
Microarray Analysis ◽  
Natural Transformation ◽  
Type Iv Pili ◽  
Transcriptional Unit ◽  
Natural Competence ◽  
Type Iv ◽  
Mutant Strains ◽  
Synechocystis Sp ◽  
Pcc 6803

Cyanobacteria synthesize type IV pili, which are known to be essential for motility, adhesion and natural competence. They consist of long flexible fibres that are primarily composed of the major pilin PilA1 in Synechocystis sp. PCC 6803. In addition, Synechocystis encodes less abundant pilin-like proteins, which are known as minor pilins. The transcription of the minor pilin genes pilA5, pilA6 and pilA9-pilA11 is inversely regulated in response to different conditions. In this study, we show that the minor pilin PilA5 is essential for natural transformation but is dispensable for motility and flocculation. In contrast, a set of minor pilins encoded by the pilA9-slr2019 transcriptional unit are necessary for motility but are dispensable for natural transformation. Neither pilA5-pilA6 nor pilA9-slr2019 are essential for pilus assembly as mutant strains showed type IV pili on the cell surface. Microarray analysis demonstrated that the transcription levels of known and newly predicted minor pilin genes change in response to surface contact. A total of 120 genes were determined to have altered transcription between planktonic and surface growth. Among these genes, 13 are located on the pSYSM plasmid. The results of our study indicate that different minor pilins facilitate distinct pilus functions.

scholarly journals Dynamic Changes of IsiA-Containing Complexes during Long-Term Iron Deficiency in Synechocystis sp. PCC 6803

2017 ◽  
Vol 10 (1) ◽  
pp. 143-154 ◽  
Author(s):  
Fei Ma ◽  
Xin Zhang ◽  
Xi Zhu ◽  
Tianpei Li ◽  
Jiao Zhan ◽  
...  
Keyword(s):  
Iron Deficiency ◽  
Dynamic Changes ◽  
Synechocystis Sp ◽  
Pcc 6803
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