Effect of Growth Regulators on CO2 Assimilation in Leaves, and its Correlation with the Bud Break Response in Photosynthesis

The present experimental study was aimed to overcome the traditional methods of propagation that limit the number of propagules by in-vitro regeneration through nodal explants of Dendrocalamus hamiltonii with a comparative study of growth regulators during the shooting and rooting process. Dendrocalamus hamiltonii is distributed from the Himalayas (Nepal) to the northern part of Burma. Collection of explants was done from different selected sites of CPTs. There was the use of HgCl2 and Ca (OCl)2 as sterilizing agents in different concentrations and its effect was visualized during the sprouting stage. Culm explants were cultured in a bottle containing White media (Wm) supplemented with BA and Kinetin for sprouting and IAA, IBA, NAA for rooting. There is also the use of IAA+IBA+NAA in combined form as a supplementary solution 0.1% HgCl2 treatment for 20-minute results into77.80% aseptic buds and 72% bud -break. Among the used growth-hormones, BA with concentration 0.25mg/l and 0.50mg/l respectively were appropriate for shoot-multiplication rate, 4.01±0.3 and 4.3±0.4 were ideal observation incorporation with BA (1.00mg/l) and BA (1.50mg/l) respectively. Maximum sprouting rate14.77±3.37with application of BA (2.00mg/l) and maximum shoot length4.3±0.4 is observed at BA (1.50mg/l). The applications of rooting hormone IAA+IBA+NAA in the concentration of 1.0 mg/l results in 72.5±0.3(rooting) and 11.1±0.3 (av. No. of the root).

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Effect of growth regulators and dormancy-breaking chemicals on bud break and yield of ‘Palomino’ grape vines

New Zealand Journal of Experimental Agriculture ◽

10.1080/03015521.1977.10425993 ◽

1977 ◽

Vol 5 (4) ◽

pp. 339-343

Author(s):

M. E. Hopping

Keyword(s):

Growth Regulators ◽

Bud Break ◽

Dormancy Breaking

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PRE-FORCING TREATMENTS AND PLANT GROWTH REGULATORS IN THE FORCING SOLUTION PROMOTE MACRO- AND MICRO-PROPAGATION OF WOODY PLANT SPECIES

HortScience ◽

10.21273/hortsci.25.9.1124d ◽

1990 ◽

Vol 25 (9) ◽

pp. 1124d-1124

Author(s):

Gouchen Yang ◽

Paul E. Read

Keyword(s):

Plant Growth ◽

Plant Growth Regulators ◽

Plant Species ◽

Growth Regulators ◽

Woody Plant ◽

Shoot Proliferation ◽

Bud Break ◽

Woody Plant Species ◽

Forcing Solution

BA, IBA and GA3 were incorporated into softwood tissues to be cultured in vitro or rooted as cuttings by adding the plant growth regulators (PGR) at various concentrations to a forcing solution containing 200 mg/l 8-hydroxyquinoline citrate and 2% sucrose. BA and GA3 helped break bud dormancy in autumn-collected stems and increased percent bud-break. IBA inhibited bud break and shoot elongation. Rooting of forced softwood cuttings was enhanced by IBA in the forcing solution, while GA3 inhibited the rooting of plant species tested. When dormant stems were forced with periodic additions of BA (10 mg/l) in the forcing solution, in vitro shoot proliferation was enhanced. However, inclusion of GA3 in the forcing solution reduced shoot proliferation. A pre-forcing NaOCl soak and a pre-forcing treatment with wetting agents accelerated bud break, size and number of shoots available for both micro- and macro-propagation of the woody plant species tested. The forcing solution protocol described is an effective PGR delivery system and it can be used by the propagator to extend the season for obtaining softwood growth suitable for use as in vitro explants or softwood cuttings.

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In-vitro Conservation of Bacopa monniera (L.) Wettst.: A Memory Booster Plant

Our Nature ◽

10.3126/on.v8i1.4311 ◽

1970 ◽

Vol 8 (1) ◽

pp. 40-47 ◽

Cited By ~ 1

Author(s):

J. Prabha ◽

U. Rani ◽

A. Sen ◽

R.N. Verma ◽

A. Batra

Keyword(s):

Growth Regulators ◽

High Frequency ◽

In Vitro Propagation ◽

Shoot Proliferation ◽

Nodal Segment ◽

Bud Break ◽

Ms Medium ◽

Bacopa Monniera ◽

In Vitro Shoot Regeneration

A mass in-vitro propagation system of Bacopa monniera (L.) Wettst., a traditional medicinal herb, has been developed. Shoot proliferation and growth were greatly influenced by the months of the year during which the explant were collected. High frequency bud break coupled with maximum number of shoots through nodal segment were found in the month of June and August. Of the different growth regulators tried, for in vitro shoot regeneration, MS medium with BAP (1.0 mg/l) induced maximum number of proliferative shoots (65.00±1.453). Multiplication and elongation of the shoots were obtained after regular sub culturing on the same medium after 15-21 days. Rooting from basal end of the shoots occurred on MS medium with the addition of IBA (0.5 mg/l). After a hardening phase of 4 weeks, almost 95% transplantation was success in the field.DOI: 10.3126/on.v8i1.4311

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