scholarly journals On the contribution of substrate flexibility to define methionine adenosyltransferase specificity

2021 ◽  
Vol 77 (a1) ◽  
pp. a80-a80
Author(s):  
Paola Laurino ◽  
Madhuri Gade ◽  
Li Lynn Tan ◽  
Mahakaran Sandhu ◽  
Andie Delaney ◽  
...  
Keyword(s):  
Methionine Adenosyltransferase

scholarly journals Liver cell proliferation requires methionine adenosyltransferase 2A mRNA up-regulation

Hepatology ◽  
2002 ◽  
Vol 35 (6) ◽  
pp. 1381-1391 ◽  
Author(s):  
Covadonga Pañeda ◽  
Itziar Gorospe ◽  
Blanca Herrera ◽  
Toshikazu Nakamura ◽  
Isabel Fabregat ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Liver Cell ◽  
Methionine Adenosyltransferase

Functional characterization of a thermostable methionine adenosyltransferase from Thermus thermophilus HB27

2016 ◽  
Vol 10 (2) ◽  
pp. 238-244 ◽  
Author(s):  
Yanhui Liu ◽  
Biqiang Chen ◽  
Zheng Wang ◽  
Luo Liu ◽  
Tianwei Tan
Keyword(s):  
Thermus Thermophilus ◽  
Functional Characterization ◽  
Methionine Adenosyltransferase

scholarly journals Insulin-like growth factor 1 activates methionine adenosyltransferase 2A transcription by multiple pathways in human colon cancer cells

2011 ◽  
Vol 436 (2) ◽  
pp. 507-516 ◽  
Author(s):  
Heping Yang ◽  
Tony W. H. Li ◽  
Jian Peng ◽  
José M. Mato ◽  
Shelly C. Lu
Keyword(s):  
Colon Cancer ◽  
Growth Factor ◽  
Protein Binding ◽  
Cancer Cells ◽  
Promoter Activity ◽  
Human Colon ◽  
Methionine Adenosyltransferase ◽  
Insulin Like Growth Factor ◽  
Colon Cancer Cells ◽  
Human Colon Cancer

We have previously reported that the expression of MAT2A (methionine adenosyltransferase 2A) is increased in human colon cancer and in colon cancer cells treated with IGF-1 (insulin-like growth factor-1), which was required for its mitogenic effect. The aim of the present study was to elucidate the molecular mechanisms of IGF-1-mediated MAT2A induction. Nuclear run-on analysis confirmed that the increase in MAT2A expression lies at the transcriptional level. DNase I footprinting of the MAT2A promoter region revealed a similar protein-binding pattern in colon cancer and IGF-1-treated RKO cells. IGF-1 induced MAT2A promoter activity and increased nuclear protein binding to USF (upstream stimulatory factor)/c-Myb, YY1 (Yin and Yang 1), E2F, AP-1 (activator protein 1) and NF-κB (nuclear factor κB) consensus elements. IGF-1 increased the expression of c-Jun, FosB, MafG, p65, c-Myb, E2F-1 and YY1 at the pre-translational level. Knockdown of p65, MafG, c-Myb or E2F-1 lowered basal MAT2A expression and blunted the inductive effect of IGF-1 on MAT2A, whereas knockdown of YY1 increased basal MAT2A expression and had no effect on IGF-1-mediated MAT2A induction. Consistently, mutation of AP-1, NF-κB, E2F and USF/c-Myb elements individually blunted the IGF-1-mediated increase in MAT2A promoter activity, and combined mutations completely prevented the increase. In conclusion, IGF-1 activates MAT2A transcription by both known and novel pathways. YY1 represses MAT2A expression.

Regulation of human methionine adenosyltransferase 2A expression by NFkB and AP-1

2003 ◽  
Vol 124 (4) ◽  
pp. A725
Author(s):  
Heping Yang ◽  
Victor Yu ◽  
Ying Zeng ◽  
Mamatha Sadda ◽  
Taunia D. Lee ◽  
...  
Keyword(s):  
Methionine Adenosyltransferase

scholarly journals Methionine Adenosyltransferase as a Useful Molecular Systematics Tool Revealed by Phylogenetic and Structural Analyses

2004 ◽  
Vol 335 (3) ◽  
pp. 693-706 ◽  
Author(s):  
Gabino F Sánchez-Pérez ◽  
José M Bautista ◽  
Marı́a A Pajares
Keyword(s):  
Molecular Systematics ◽  
Methionine Adenosyltransferase

Molecular cloning and sequence analysis of methionine adenosyltransferase from the economic seaweed Undaria pinnatifida

2012 ◽  
Vol 25 (1) ◽  
pp. 81-87 ◽  
Author(s):  
Kun Qiao ◽  
Shi Guo Li ◽  
Hong Yan Li ◽  
Shao Ming Tong ◽  
He Sheng Hou
Keyword(s):  
Sequence Analysis ◽  
Molecular Cloning ◽  
Undaria Pinnatifida ◽  
Methionine Adenosyltransferase

scholarly journals Understanding molecular recognition of promiscuity of thermophilic methionine adenosyltransferase sMAT fromSulfolobus solfataricus

FEBS Journal ◽  
2014 ◽  
Vol 281 (18) ◽  
pp. 4224-4239 ◽  
Author(s):  
Fengbin Wang ◽  
Shanteri Singh ◽  
Jianjun Zhang ◽  
Tyler D. Huber ◽  
Kate E. Helmich ◽  
...  
Keyword(s):  
Molecular Recognition ◽  
Methionine Adenosyltransferase
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