Structure of the plant growth-promoting factor YxaL from the rhizobacterium Bacillus velezensis and its application to protein engineering

2022 ◽  
Vol 78 (1) ◽  
Author(s):  
Jiheon Kim ◽  
Ha Pham ◽  
Yeongjin Baek ◽  
Inseong Jo ◽  
Yong-Hak Kim ◽  
...  
Keyword(s):  
Protein Engineering ◽  
Plant Growth ◽  
Root Growth ◽  
Structural Stability ◽  
Structural Features ◽  
Bacillus Velezensis ◽  
Structural Variations ◽  
The Third ◽  
Plant Growth Promoting ◽  
Growth Promoting

The YxaL protein was isolated from the soil bacterium Bacillus velezensis and has been shown to promote the root growth of symbiotic plants. YxaL has further been suggested to act as an exogenous signaling protein to induce the growth and branching of plant roots. Amino acid sequence analysis predicted YxaL to exhibit an eight-bladed β-propeller fold stabilized by six tryptophan-docking motifs and two modified motifs. Protein engineering to improve its structural stability is needed to increase the utility of YxaL as a plant growth-promoting factor. Here, the crystal structure of YxaL from B. velezensis was determined at 1.8 Å resolution to explore its structural features for structure-based protein engineering. The structure showed the typical eight-bladed β-propeller fold with structural variations in the third and fourth blades, which may decrease the stability of the β-propeller fold. Engineered proteins targeting the modified motifs were subsequently created. Crystal structures of the engineered YxaL proteins showed that the typical tryptophan-docking interaction was restored in the third and fourth blades, with increased structural stability, resulting in improved root growth-promoting activity in Arabidopsis seeds. The work is an example of structure-based protein engineering to improve the structural stability of β-propellor fold proteins.

scholarly journals Bacillus velezensisWall Teichoic Acids Are Required for Biofilm Formation and Root Colonization

2018 ◽  
Vol 85 (5) ◽  
Author(s):  
Zhihui Xu ◽  
Huihui Zhang ◽  
Xinli Sun ◽  
Yan Liu ◽  
Wuxia Yan ◽  
...  
Keyword(s):  
Plant Growth ◽  
Biofilm Formation ◽  
Root Colonization ◽  
Molecular Networks ◽  
Model Organisms ◽  
Bacillus Velezensis ◽  
Content Type ◽  
Plant Growth Promoting ◽  
Colonization Process ◽  
Growth Promoting

ABSTRACTRhizosphere colonization by plant growth-promoting rhizobacteria (PGPR) along plant roots facilitates the ability of PGPR to promote plant growth and health. Thus, an understanding of the molecular mechanisms of the root colonization process by plant-beneficialBacillusstrains is essential for the use of these strains in agriculture. Here, we observed that ansfpgene mutant of the plant growth-promoting rhizobacteriumBacillus velezensisSQR9 was unable to form normal biofilm architecture, and differential protein expression was observed by proteomic analysis. A minor wall teichoic acid (WTA) biosynthetic protein, GgaA, was decreased over 4-fold in the Δsfpmutant, and impairment of theggaAgene postponed biofilm formation and decreased cucumber root colonization capabilities. In addition, we provide evidence that the major WTA biosynthetic enzyme GtaB is involved in both biofilm formation and root colonization. The deficiency in biofilm formation of the ΔgtaBmutant may be due to an absence of UDP-glucose, which is necessary for the synthesis of biofilm matrix exopolysaccharides (EPS). These observations provide insights into the root colonization process by a plant-beneficialBacillusstrain, which will help improve its application as a biofertilizer.IMPORTANCEBacillus velezensisis a Gram-positive plant-beneficial bacterium which is widely used in agriculture. Additionally,Bacillusspp. are some of the model organisms used in the study of biofilms, and as such, the molecular networks and regulation systems of biofilm formation are well characterized. However, the molecular processes involved in root colonization by plant-beneficialBacillusstrains remain largely unknown. Here, we showed that WTAs play important roles in the plant root colonization process. The loss of thegtaBgene affects the ability ofB. velezensisSQR9 to sense plant polysaccharides, which are important environmental cues that trigger biofilm formation and colonization in the rhizosphere. This knowledge provides new insights into theBacillusroot colonization process and can help improve our understanding of plant-rhizobacterium interactions.

scholarly journals Antimicrobial, plant growth-promoting and genomic properties of the peanut endophyte Bacillus velezensis LDO2

2019 ◽  
Vol 218 ◽  
pp. 41-48 ◽  
Author(s):  
Liang Chen ◽  
Hao Shi ◽  
Junying Heng ◽  
Dianxuan Wang ◽  
Ke Bian
Keyword(s):  
Plant Growth ◽  
Bacillus Velezensis ◽  
Plant Growth Promoting ◽  
Growth Promoting

scholarly journals Plant growth-promoting activity of beta-propeller protein YxaL secreted from Bacillus velezensis strain GH1-13

PLoS ONE ◽  
2019 ◽  
Vol 14 (4) ◽  
pp. e0207968 ◽  
Author(s):  
Yong-Hak Kim ◽  
Yunhee Choi ◽  
Yu Yeong Oh ◽  
Nam-Chul Ha ◽  
Jaekyeong Song
Keyword(s):  
Plant Growth ◽  
Bacillus Velezensis ◽  
Plant Growth Promoting ◽  
Plant Growth Promoting Activity ◽  
Growth Promoting

Nitric Oxide and Plant Growth Promoting Rhizobacteria: Common Features Influencing Root Growth and Development

2007 ◽  
pp. 1-33 ◽  
Author(s):  
Celeste Molina‐Favero ◽  
Cecilia Mónica Creus ◽  
María Luciana Lanteri ◽  
Natalia Correa‐Aragunde ◽  
María Cristina Lombardo ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Plant Growth ◽  
Root Growth ◽  
Growth And Development ◽  
Plant Growth Promoting Rhizobacteria ◽  
Common Features ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Root Growth And Development

scholarly journals Genome Mining of Three Plant Growth-Promoting Bacillus Species from Maize Rhizosphere

2021 ◽  
Author(s):  
Oluwaseyi Samuel Olanrewaju ◽  
Modupe Stella Ayilara ◽  
Ayansina Segun Ayangbenro ◽  
Olubukola Oluranti Babalola
Keyword(s):  
Bacillus Subtilis ◽  
Plant Growth ◽  
Bacillus Species ◽  
Bacillus Velezensis ◽  
Growth Promoters ◽  
Genetic Elements ◽  
Pan Genome ◽  
Genetic Components ◽  
Plant Growth Promoting ◽  
Growth Promoting

AbstractBacillus species genomes are rich in plant growth-promoting genetic elements. Bacillus subtilis and Bacillus velezensis are important plant growth promoters; hence, to further improve their abilities, the genetic elements responsible for these traits were characterized and reported. Genetic elements reported include those of auxin, nitrogen fixation, siderophore production, iron acquisition, volatile organic compounds, and antibiotics. Furthermore, the presence of phages and antibiotic-resistant genes in the genomes are reported. Pan-genome analysis was conducted using ten Bacillus species. From the analysis, pan-genome of Bacillus subtilis and Bacillus velezensis are still open. Ultimately, this study brings an insight into the genetic components of the plant growth-promoting abilities of these strains and shows their potential biotechnological applications in agriculture and other relevant sectors.

scholarly journals Plantibacter flavus, Curtobacterium herbarum, Paenibacillus taichungensis, and Rhizobium selenitireducens Endophytes Provide Host-Specific Growth Promotion of Arabidopsis thaliana, Basil, Lettuce, and Bok Choy Plants

2019 ◽  
Vol 85 (19) ◽  
Author(s):  
Evan Mayer ◽  
Patricia Dörr de Quadros ◽  
Roberta Fulthorpe
Keyword(s):  
Arabidopsis Thaliana ◽  
Plant Growth ◽  
Root Growth ◽  
Plant Growth Promotion ◽  
Growth Promotion ◽  
Plant Growth Promoting Bacteria ◽  
Bacterial Endophytes ◽  
Content Type ◽  
Plant Growth Promoting ◽  
Growth Promoting

ABSTRACT A collection of bacterial endophytes isolated from stem tissues of plants growing in soils highly contaminated with petroleum hydrocarbons were screened for plant growth-promoting capabilities. Twenty-seven endophytic isolates significantly improved the growth of Arabidopsis thaliana plants in comparison to that of uninoculated control plants. The five most beneficial isolates, one strain each of Curtobacterium herbarum, Paenibacillus taichungensis, and Rhizobium selenitireducens and two strains of Plantibacter flavus were further examined for growth promotion in Arabidopsis, lettuce, basil, and bok choy plants. Host-specific plant growth promotion was observed when plants were inoculated with the five bacterial strains. P. flavus strain M251 increased the total biomass and total root length of Arabidopsis plants by 4.7 and 5.8 times, respectively, over that of control plants and improved lettuce and basil root growth, while P. flavus strain M259 promoted Arabidopsis shoot and root growth, lettuce and basil root growth, and bok choy shoot growth. A genome comparison between P. flavus strains M251 and M259 showed that both genomes contain up to 70 actinobacterial putative plant-associated genes and genes involved in known plant-beneficial pathways, such as those for auxin and cytokinin biosynthesis and 1-aminocyclopropane-1-carboxylate deaminase production. This study provides evidence of direct plant growth promotion by Plantibacter flavus. IMPORTANCE The discovery of new plant growth-promoting bacteria is necessary for the continued development of biofertilizers, which are environmentally friendly and cost-efficient alternatives to conventional chemical fertilizers. Biofertilizer effects on plant growth can be inconsistent due to the complexity of plant-microbe interactions, as the same bacteria can be beneficial to the growth of some plant species and neutral or detrimental to others. We examined a set of bacterial endophytes isolated from plants growing in a unique petroleum-contaminated environment to discover plant growth-promoting bacteria. We show that strains of Plantibacter flavus exhibit strain-specific plant growth-promoting effects on four different plant species.

scholarly journals Draft Genome Sequence of Bacillus velezensis PEBA20, a Strain with a Plant Growth-Promoting Effect and Biocontrol Potential

2018 ◽  
Vol 6 (21) ◽  
Author(s):  
Wei-Jian Kong ◽  
Yong-Cai Yan ◽  
Xiang-Ying Li ◽  
Zhen-Yu Liu
Keyword(s):  
Plant Growth ◽  
Genome Sequence ◽  
Draft Genome ◽  
Draft Genome Sequence ◽  
Bacillus Velezensis ◽  
Promoting Effect ◽  
Content Type ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Biocontrol Potential

ABSTRACT Bacillus velezensis PEBA20 is a poplar endophyte with biocontrol activities and plant growth-promoting effects. The genome of B. velezensis PEBA20 was sequenced and the draft genome assembled, with a length of 4,249,176 bp and 4,487 genes.

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