Noninvasive cellular quantity measurement in Bone Marrow Stromal Cells/ Beta-tricalcium phosphate

Author(s):  
Naomi Yagi ◽  
Tomomoto Ishikawa ◽  
Kei Kuramoto ◽  
Syoji Kobashi ◽  
Yutaka Hata
2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Xuehui Zhang ◽  
Mingming Xu ◽  
Xinggang Liu ◽  
Feng Zhang ◽  
Yan Wei ◽  
...  

Nano-β-tricalcium phosphate/collagen (n-β-TCP/Col) is considered with good osteoconductivity. However, the therapeutic effectiveness of n-β-TCP/Col scaffolds in combination with autologous bone marrow stromal cells (BMSCs) remains to be elucidated for the repair of critical-sized bone defects. In this study, we found that n-β-TCP/Col scaffolds exhibited high biocompatibilityin vitro. The introduction of BMSCs expandedin vitroto the scaffolds dramatically enhanced their efficiency to restore critical-sized bone defects, especially during the initial stage after implantation. Collectively, these results suggest that autologous BMSCs in n-β-TCP/Col scaffolds have the potential to be applied in bone tissue engineering.


2008 ◽  
Vol 368-372 ◽  
pp. 1235-1237 ◽  
Author(s):  
Lei Liu ◽  
Run Liang Chen ◽  
Yun Feng Lin ◽  
Wei Dong Tian ◽  
Sheng Wei Li

Hydroxyapatite-tricalcium phosphate (HA-TCP) is a new kind of material which shows good biocompatibility, biological degradability, and porosity. This study aimed to determine the effectiveness of HA-TCP as a bone tissue engineering scaffold. In this study, critical size cranial defects were reconstructed with compounds of autogenous bone marrow stromal cells (BMSCs) and HA-TCP. The resulting grafts were examined by X-ray, histological examination, semi-quantitative analysis of osteogenesis, immunochemical examination (collagen type I and III), scanning electron microscopy and transmission electron microscopy. The results showed that HA-TCP is a good bone tissue engineering scaffold and BMSCs/HA-TCP is a promising technique for reconstruction of bone defects.


2009 ◽  
Vol 15 (7) ◽  
pp. 1489-1499 ◽  
Author(s):  
Ichiro Torigoe ◽  
Shinichi Sotome ◽  
Akio Tsuchiya ◽  
Toshitaka Yoshii ◽  
Hidetsugu Maehara ◽  
...  

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