Visible light-based stereolithography bioprinting of cell-adhesive gelatin hydrogels

2017 ◽  
Author(s):  
Zongjie Wang ◽  
Zhenlin Tian ◽  
Xian Jin ◽  
Jonathan F. Holzman ◽  
Frederic Menard ◽  
...  
Keyword(s):  
Visible Light ◽  
Cell Adhesive

Visible Light Photoinitiation of Cell-Adhesive Gelatin Methacryloyl Hydrogels for Stereolithography 3D Bioprinting

2018 ◽  
Vol 10 (32) ◽  
pp. 26859-26869 ◽  
Author(s):  
Zongjie Wang ◽  
Hitendra Kumar ◽  
Zhenlin Tian ◽  
Xian Jin ◽  
Jonathan F. Holzman ◽  
...  
Keyword(s):  
Visible Light ◽  
3D Bioprinting ◽  
Cell Adhesive

Studies of metaphase chromosomes in the scanning transmission x-ray microscope: Image fidelity, radiation damage and specimen preparation

1992 ◽  
Vol 50 (2) ◽  
pp. 1038-1039
Author(s):  
Shawn Williams ◽  
Xiaodong Zhang ◽  
Susan Lamm ◽  
Jack Van’t Hof
Keyword(s):  
Visible Light ◽  
Radiation Damage ◽  
Cross Section ◽  
Imaging Techniques ◽  
Dose Range ◽  
Specimen Preparation ◽  
X Rays ◽  
Metaphase Chromosomes ◽  
X Ray ◽  
Scanning Transmission

The Scanning Transmission X-ray Microscope (STXM) is well suited for investigating metaphase chromosome structure. The absorption cross-section of soft x-rays having energies between the carbon and oxygen K edges (284 - 531 eV) is 6 - 9.5 times greater for organic specimens than for water, which permits one to examine unstained, wet biological specimens with resolution superior to that attainable using visible light. The attenuation length of the x-rays is suitable for imaging micron thick specimens without sectioning. This large difference in cross-section yields good specimen contrast, so that fewer soft x-rays than electrons are required to image wet biological specimens at a given resolution. But most imaging techniques delivering better resolution than visible light produce radiation damage. Soft x-rays are known to be very effective in damaging biological specimens. The STXM is constructed to minimize specimen dose, but it is important to measure the actual damage induced as a function of dose in order to determine the dose range within which radiation damage does not compromise image quality.

Scanning luminescence x-ray microscopy: Progress toward selective staining using microspheres

1994 ◽  
Vol 52 ◽  
pp. 74-75
Author(s):  
C. Jacobsen ◽  
J. Fu ◽  
S. Mayer ◽  
Y. Wang ◽  
S. Williams
Keyword(s):  
Visible Light ◽  
Active Sites ◽  
Light Emission ◽  
Chinese Hamster ◽  
Polystyrene Spheres ◽  
Good Resistance ◽  
X Ray ◽  
Selective Staining ◽  
Visible Light Emission ◽  
Specific Labelling

In scanning luminescence x-ray microscopy (SLXM), a high resolution x-ray probe is used to excite visible light emission (see Figs. 1 and 2). The technique has been developed with a goal of localizing dye-tagged biochemically active sites and structures at 50 nm resolution in thick, hydrated biological specimens. Following our initial efforts, Moronne et al. have begun to develop probes based on biotinylated terbium; we report here our progress towards using microspheres for tagging.Our initial experiments with microspheres were based on commercially-available carboxyl latex spheres which emitted ~ 5 visible light photons per x-ray absorbed, and which showed good resistance to bleaching under x-ray irradiation. Other work (such as that by Guo et al.) has shown that such spheres can be used for a variety of specific labelling applications. Our first efforts have been aimed at labelling ƒ actin in Chinese hamster ovarian (CHO) cells. By using a detergent/fixative protocol to load spheres into cells with permeabilized membranes and preserved morphology, we have succeeded in using commercial dye-loaded, spreptavidin-coated 0.03μm polystyrene spheres linked to biotin phalloidon to label f actin (see Fig. 3).

Photocatalyst-free decarboxylative aminoalkylation of imidazo[1,2-a]pyridines with N-aryl glycines enabled by visible light

2019 ◽  
Vol 6 (21) ◽  
pp. 3693-3697 ◽  
Author(s):  
Jiu-Jian Ji ◽  
Zhi-Qiang Zhu ◽  
Li-Jin Xiao ◽  
Dong Guo ◽  
Xiao Zhu ◽  
...  
Keyword(s):  
Visible Light

A novel, green and efficient visible-light-promoted decarboxylative aminoalkylation reaction of imidazo[1,2-a]pyridines with N-aryl glycines has been described.

Space Charge Formation by Irradiation of Visible Light in Polyimide under DC Electric Stress

2009 ◽  
Vol 129 (7) ◽  
pp. 463-469 ◽  
Author(s):  
Tomo Tadokoro ◽  
Takuo Motoyama ◽  
Hiroshi Harada ◽  
Yasuhiro Tanaka ◽  
Tastuo Takada ◽  
...  
Keyword(s):  
Visible Light ◽  
Space Charge ◽  
Charge Formation ◽  
Electric Stress

High Performance Visible-light-responsive TiO2/CuxO/Cu Photocatalyst

2016 ◽  
Vol 136 (8) ◽  
pp. 551-552
Author(s):  
Sumio Kogoshi ◽  
Nao Kato ◽  
Yu Katsui ◽  
Noboru Katayama ◽  
Syota Yazawa ◽  
...  
Keyword(s):  
Visible Light ◽  
High Performance
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