Mean scatterer spacing of backscattered ultrasound signals from in vitro human cancellous bone specimens

2003 ◽  
Author(s):  
W.C.A. Pereira ◽  
S.L. Bridal ◽  
A. Coron ◽  
P. Laugier
Keyword(s):  
Cancellous Bone ◽  
Human Cancellous Bone ◽  
Ultrasound Signals

scholarly journals Human Endothelial-Like Differentiated Precursor Cells Maintain Their Endothelial Characteristics When Cocultured with Mesenchymal Stem Cell and Seeded onto Human Cancellous Bone

2013 ◽  
Vol 2013 ◽  
pp. 1-12 ◽  
Author(s):  
Dirk Henrich ◽  
Kerstin Wilhelm ◽  
Joerg Warzecha ◽  
Johannes Frank ◽  
John Barker ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cancellous Bone ◽  
Metabolic Activity ◽  
Bone Healing ◽  
Culture Conditions ◽  
Gene Expressions ◽  
Human Cancellous Bone ◽  
Osteogenic Gene Expression ◽  
Stimulation Of

Introduction. Cancellous bone is frequently used for filling bone defects in a clinical setting. It provides favourable conditions for regenerative cells such as MSC and early EPC. The combination of MSC and EPC results in superior bone healing in experimental bone healing models.Materials and Methods. We investigated the influence of osteogenic culture conditions on the endothelial properties of early EPC and the osteogenic properties of MSC when cocultured on cancellous bone. Additionally, cell adhesion, metabolic activity, and differentiation were assessed 2, 6, and 10 days after seeding.Results. The number of adhering EPC and MSC decreased over time; however the cells remained metabolically active over the 10-day measurement period. In spite of a decline of lineage specific markers, cells maintained their differentiation to a reduced level. Osteogenic stimulation of EPC caused a decline but not abolishment of endothelial characteristics and did not induce osteogenic gene expression. Osteogenic stimulation of MSC significantly increased their metabolic activity whereas collagen-1αand alkaline phosphatase gene expressions declined. When cocultured with EPC, MSC’s collagen-1αgene expression increased significantly.Conclusion. EPC and MSC can be coculturedin vitroon cancellous bone under osteogenic conditions, and coculturing EPC with MSC stabilizes the latter’s collagen-1αgene expression.

Propagation of two longitudinal waves in human cancellous bone: An in vitro study

2009 ◽  
Vol 125 (5) ◽  
pp. 3460 ◽  
Author(s):  
Katsunori Mizuno ◽  
Mami Matsukawa ◽  
Takahiko Otani ◽  
Pascal Laugier ◽  
Frédéric Padilla
Keyword(s):  
Cancellous Bone ◽  
In Vitro Study ◽  
Vitro Study ◽  
Longitudinal Waves ◽  
Human Cancellous Bone

Ultrasonic characteristics of in vitro human cancellous bone

2006 ◽  
Vol 120 (5) ◽  
pp. 3242-3242
Author(s):  
Isao Mano ◽  
Tadahito Yamamoto ◽  
Hiroshi Hagino ◽  
Ryota Teshima ◽  
Toshiyuki Tsujimoto ◽  
...  
Keyword(s):  
Cancellous Bone ◽  
Human Cancellous Bone

scholarly journals Ibudilast Mitigates Delayed Bone Healing Caused by Lipopolysaccharide by Altering Osteoblast and Osteoclast Activity

2021 ◽  
Vol 22 (3) ◽  
pp. 1169
Author(s):  
Yuhan Chang ◽  
Chih-Chien Hu ◽  
Ying-Yu Wu ◽  
Steve W. N. Ueng ◽  
Chih-Hsiang Chang ◽  
...  
Keyword(s):  
Cell Wall ◽  
Bone Formation ◽  
Cancellous Bone ◽  
Bone Healing ◽  
Bone Bridge ◽  
Cell Wall Components ◽  
Osteoclast Activity ◽  
Wall Components

Bacterial infection in orthopedic surgery is challenging because cell wall components released after bactericidal treatment can alter osteoblast and osteoclast activity and impair fracture stability. However, the precise effects and mechanisms whereby cell wall components impair bone healing are unclear. In this study, we characterized the effects of lipopolysaccharide (LPS) on bone healing and osteoclast and osteoblast activity in vitro and in vivo and evaluated the effects of ibudilast, an antagonist of toll-like receptor 4 (TLR4), on LPS-induced changes. In particular, micro-computed tomography was used to reconstruct femoral morphology and analyze callus bone content in a femoral defect mouse model. In the sham-treated group, significant bone bridge and cancellous bone formation were observed after surgery, however, LPS treatment delayed bone bridge and cancellous bone formation. LPS inhibited osteogenic factor-induced MC3T3-E1 cell differentiation, alkaline phosphatase (ALP) levels, calcium deposition, and osteopontin secretion and increased the activity of osteoclast-associated molecules, including cathepsin K and tartrate-resistant acid phosphatase in vitro. Finally, ibudilast blocked the LPS-induced inhibition of osteoblast activation and activation of osteoclast in vitro and attenuated LPS-induced delayed callus bone formation in vivo. Our results provide a basis for the development of a novel strategy for the treatment of bone infection.

scholarly journals Effect of synthetic bone replacement material of different size on shear stress resistance within impacted native and thermodisinfected cancellous bone: an in vitro femoral impaction bone grafting model

2021 ◽  
Author(s):  
C. Fölsch ◽  
P. Sahm ◽  
C. A. Fonseca Ulloa ◽  
G. A. Krombach ◽  
M. Kampschulte ◽  
...  
Keyword(s):  
Cancellous Bone ◽  
Bone Grafting ◽  
Shear Force ◽  
Distal Region ◽  
Impaction Bone Grafting ◽  
Proximal Point ◽  
Local Antibiotics ◽  
Joint Replacements ◽  
Native Bone

AbstractAntibiotic carrier particles of variable size might influence mechanic properties within impacted thermodisinfected and native cancellous bone different. Herafill®G containing calciumsulfate and calciumcarbonate provides high local concentrations of gentamicin being important for revision surgery in infected joint replacements. Native and thermodisinfected cancellous bone derived from 6 to 7 months old piglets was used for in vitro impaction bone grafting and supplemented each with Herafill®G granules of two different sizes. Micromovement of implants related to shear force was measured in 29 specimens distributed in 6 groups. Thermodisinfected cancellous bone revealed a significant higher shear force resistance than native bone with a mean difference of 423.8 mdeg/Nm (p < 0.001) ranging within 95% confidence interval from 181.5 to 666.0 mdeg/Nm. Adding small granules to thermodisinfected bone did not reduce shear force resistance significantly since adding large granules to native bone improved it by 344.0 mdeg/Nm (p < 0.003). Shear force resistance was found higher at the distal region of the implant compared to a proximal point of measurement throughout all specimens. Less impaction impulses were necessary for thermodisinfected bone. Thermodisinfected cancellous bone might achieve a higher degree of impaction compared with native bone resulting in increased resistance against shear force since impaction was found increased distally. Supplementation of thermodisinfected bone with small granules of Herafill®G might be considered for application of local antibiotics. Large granules appeared more beneficial for supplementation of native bone. Heterogeneity of bone graft and technical aspects of the impaction procedure have to be considered regarding the reproducibility of femoral impaction bone grafting.

Primary Stability of Short Implants Inserted Using Piezoelectric or Drilling Systems: An In Vitro Comparison

2019 ◽  
Vol 45 (4) ◽  
pp. 259-266
Author(s):  
Claudio Stacchi ◽  
Matteo De Biasi ◽  
Lucio Torelli ◽  
Massimo Robiony ◽  
Roberto Di Lenarda ◽  
...  
Keyword(s):  
Cancellous Bone ◽  
Operative Time ◽  
Primary Stability ◽  
Site Preparation ◽  
Preparation Technique ◽  
Implant Stability ◽  
Implant Stability Quotient ◽  
Custom Made ◽  
Implant Site Preparation

The primary objective of the present in vitro study was to evaluate the influence of implant site preparation technique (drills vs ultrasonic instrumentation) on the primary stability of short dental implants with two different designs inserted in simulated low-quality cancellous bone. Eighty implant sites were prepared in custom-made solid rigid polyurethane blocks with two different low cancellous bone densities (5 or 15 pounds per cubic foot [PCF]), equally distributed between piezoelectric (Surgysonic Moto, Esacrom, Italy) and conventional drilling techniques. Two short implant systems (Prama and Syra, Sweden & Martina) were tested by inserting 40 fixtures of each system (both 6.0 mm length and 5.0 mm diameter), divided in the four subgroups (drills/5 PCF density; drills/15 PCF density; piezo/5 PCF density; piezo/15 PCF density). Insertion torque (Ncm), implant stability quotient values, removal torque (Ncm), and surgical time were recorded. Data were analyzed by 3-way ANOVA and Scheffé's test (α = 0.05). With slight variations among the considered dependent variables, overall high primary implant stability was observed across all subgroups. Piezoelectric instrumentation allowed for comparable or slightly superior primary stability in comparison with the drilling procedures in both implant systems. The Prama implants group showed the highest mean reverse torque and Syra implants the highest implant stability quotient values. Piezoelectric implant site preparation took prolonged operative time compared to conventional preparation with drills; among the drilling procedures, Syra system required fewer surgical steps and shorter operative time.

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