Aspergillus spp. eliminate Sclerotinia sclerotiorum by imbalancing the ambient oxalic acid concentration and parasitizing its sclerotia

2020 ◽  
Vol 22 (12) ◽  
pp. 5265-5279
Author(s):  
Osama Atallah ◽  
Sherene Yassin
Author(s):  
Natalia Stepanova ◽  
Lyudmyla Snisar ◽  
Larysa Lebid ◽  
Victoria Driianska

Abstract. In the present exploratory cross-sectional cohort study, we evaluated whether plasma and urine oxalate concentrations in patients with primary glomerulonephritis depend not only on the glomerular filtration rate but also on the proteinuria level and influence the inflammatory response. Methods. We enrolled 100 participants, including 76 patients with glomerulonephritis having chronic kidney disease stage (CKD) 1–3b (69.7% of them with nephrotic syndrome) and 24 healthy volunteers. We excluded patients with diabetes, cardiovascular disease and those with glomerulonephritis with an estimated GFR (eGFR) < 30 mL/min/1.73 m2. In addition to routine hematological and biochemical tests, plasma oxalate concentration, urinary oxalate excretion, and serum interleukin (IL)-6 and monocyte chemoattractant protein-1 (MCP-1) levels were assessed in all study participants. Results. We observed that plasma oxalic acid concentration was significantly higher in patients with glomerulonephritis (19.0 [5.9–45.2] µmol/L) than in healthy volunteers (5.5 [3.8–7.3] µmol/L, p < 0.0001). Moreover, nephrotic proteinuria was significantly associated with plasma oxalic acid elevation independent of the patients’ age, sex, glomerular filtration rate, and body mass index (odds ratio = 1.42, 95% confidence interval = 1.13–1.77, p = 0.002). In turn, the increased plasma oxalic acid concentration was associated with high levels of serum IL-6 and MCP-1, which may be cardiovascular risk factors in patients with primary glomerulonephritis. Conclusions. Nephrotic proteinuria was significantly associated with the elevation of plasma oxalic acid concentration and hyperoxaluria in glomerulonephritis patients with CKD stages 1–3b. Plasma oxalate at least partly promotes inflammation, which may be a cardiovascular risk factor in patients with glomerulonephritis in the early stages of CKD. Future studies should recruit at least 156 participants to confirm our preliminary results, validate nephrotic proteinuria as a risk factor for oxalate metabolism violation or determine the role of impaired oxalate homeostasis in clinical outcomes in patients with glomerulonephritis.


2021 ◽  
Vol 4 (2) ◽  
pp. 39-45
Author(s):  
Illah Sailah ◽  
Erliza Hambali ◽  
Fadilla Eka Aulyana

The development of bio degreaser made from palm oil surfactant aims to substitute bio degreaser made from petroleum surfactant which is less environmentally friendly. The development was carried out by formulating palm methyl ester sulfonate (MES) surfactant with oxalic acid as metal or non-metal cleaning agent. The purpose of this study was to obtain the best concentration of oxalic acid in the bio degreaser formulation. The concentrations of oxalic acid tested were 7, 8, and 9%. The best concentration of oxalic acid was determined based on the results of characteristic tests and detergency tests, namely 7% oxalic acid concentration. The resulting bio degreaser product has a pH of 1.6; viscosity 1.39 cp; specific gravity of 1.012; surface tension 32 dyne/cm and detergency power 84%. Furthermore, the resulting bio degreaser was added with Diethanolamioda (DEA) surfactant. The purpose of adding DEA surfactant is to increase the pH and lower the surface tension. The formulation results showed an increase in pH from 1.6 to 3.2 and a decrease in surface tension from 31.97 dyne/cm to 28.70 dyne/cm. In addition, there was an increase in viscosity from 1.39 cp to 1.62 cp and specific gravity from 1.012 to 1.018.


2019 ◽  
Vol 23 (10) ◽  
pp. 2951-2959 ◽  
Author(s):  
Dewei Liu ◽  
Peng Yang ◽  
Haiyang Dai ◽  
Tao Li ◽  
Renzhong Xue ◽  
...  

2021 ◽  
Vol 2 (2) ◽  
pp. 39-45
Author(s):  
Illah Sailah ◽  
Erliza Hambali ◽  
Fadilla Eka Aulyana

The development of bio degreaser made from palm oil surfactant aims to substitute bio degreaser made from petroleum surfactant which is less environmentally friendly. The development was carried out by formulating palm methyl ester sulfonate (MES) surfactant with oxalic acid as metal or non-metal cleaning agent. The purpose of this study was to obtain the best concentration of oxalic acid in the bio degreaser formulation. The concentrations of oxalic acid tested were 7, 8, and 9%. The best concentration of oxalic acid was determined based on the results of characteristic tests and detergency tests, namely 7% oxalic acid concentration. The resulting bio degreaser product has a pH of 1.6; viscosity 1.39 cp; specific gravity of 1.012; surface tension 32 dyne/cm and detergency power 84%. Furthermore, the resulting bio degreaser was added with Diethanolamioda (DEA) surfactant. The purpose of adding DEA surfactant is to increase the pH and lower the surface tension. The formulation results showed an increase in pH from 1.6 to 3.2 and a decrease in surface tension from 31.97 dyne/cm to 28.70 dyne/cm. In addition, there was an increase in viscosity from 1.39 cp to 1.62 cp and specific gravity from 1.012 to 1.018.


Author(s):  
HERMAN SURYAD ◽  
ARRY YANUAR ◽  
HARMITA ◽  
PUTRI WINNY RACHMADANI

Objective: The study aimed to identify the best conditions using oxalic acid for hydrolysis of hemicellulose in oil palm empty fruit bunch (OPEFB)biomass.Methods: The analytical method of high-performance liquid chromatography (HPLC) was using a SUPELCOSIL LC-NH2 column, refractive indexdetection detector, and three compositions of the mobile phase. At first, the hydrolysis of hemicellulose in OPEFB powder was optimized by applyinga response surface methodology. A three-variable, six-central composite design was used for the experiments. Temperature (between 95°C and135°C), reaction time (between 10 and 110 min), and oxalic acid concentration (between 1% and 7% [w/v]) were evaluated by running 15 differentexperiments at constant biomass concentrations. Then, hydrolysis was optimized again at the constant temperature selected with three variables:OPEFB concentration, reaction time, and oxalic acid concentration. Hydrolysate samples were detoxified with carbon active, and furfural compoundwas analyzed by gas chromatography with flame ionization detector.Results: The optimum condition of HPLC was using acetonitrile: water (9:1) at a flow rate of 1.0 ml/min. The first hydrolysis results showeda high yield of D-xylose produced, which was 6.40 g D-xylose/100 g OPEFB biomass, with a xylose recovery of 93.8%. However, this result wasnot yet optimum. Further hydrolysis at constant temperature experiment produced the highest xylose yield of 13.13%, equivalent to 32 g/lD-xylose.Conclusion: The yield of D-xylose from mild hydrolysis using oxalic acid was similar to that using dilute sulfuric acid as used in the previous studyby Rahman et al.


Sign in / Sign up

Export Citation Format

Share Document