scholarly journals Differential Regulation of Decorin and Biglycan Gene Expression by Dexamethasone and Retinoic Acid in Cultured Human Skin Fibroblasts

1995 ◽  
Vol 104 (4) ◽  
pp. 503-508 ◽  
Author(s):  
Veli-Matti Kähäri ◽  
Lari Häkkinen ◽  
Jukka Westermarck ◽  
Hannu Larjava
Keyword(s):  
Gene Expression ◽  
Retinoic Acid ◽  
Human Skin ◽  
Differential Regulation ◽  
Skin Fibroblasts ◽  
Human Skin Fibroblasts

scholarly journals Interleukin-1β prevents the stimulatory effect of transforming growth factor-β on collagen gene expression in human skin fibroblasts

1990 ◽  
Vol 271 (3) ◽  
pp. 827-830 ◽  
Author(s):  
J Heino ◽  
T Heinonen
Keyword(s):  
Gene Expression ◽  
Human Skin ◽  
Skin Fibroblasts ◽  
Collagen Gene ◽  
Mrna Levels ◽  
Tgf Beta ◽  
Human Skin Fibroblasts ◽  
Collagen Mrna ◽  
Collagen Gene Expression ◽  
Beta 2

Transforming growth factors beta 1 and beta 2 (TGF-beta 1 and TGF-beta 2) are well-characterized strong inducers of collagen gene expression. A 100 pM concentration of TGF-beta 1 or TGF-beta 2 increases pro alpha 1(I) collagen mRNA levels in human skin fibroblasts 6.6-fold and 7.0-fold respectively, and also increases the accumulation of procollagens in the cell culture medium. Interleukin-1 beta (IL-1 beta) is an inflammatory mediator which also regulates connective tissue metabolism. A small concentration of IL-1 beta (0.01-1.0 unit/ml) slightly increases pro alpha 1(I) collagen mRNA levels (2.2-fold). Here we provide evidence that IL-1 beta prevents the stimulatory effect of TGFs-beta on collagen synthesis in human skin fibroblasts. An IL-1 beta concentration of 1 unit/ml is enough to keep pro alpha 1(I) collagen mRNA levels at control values in cells stimulated by 100 pM-TGF-beta 1. Thus the results indicate that IL-1 beta inhibits collagen synthesis in cells activated by TGFs-beta, whereas it does not significantly change or might even stimulate collagen gene expression in non-activated cells.

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