scholarly journals A novel method for direct measurement of complement convertases activity in human serum

2014 ◽  
Vol 178 (1) ◽  
pp. 142-153 ◽  
Author(s):  
A. M. Blom ◽  
E. B. Volokhina ◽  
V. Fransson ◽  
P. Strömberg ◽  
L. Berghard ◽  
...  
Keyword(s):  
Human Serum ◽  
Direct Measurement ◽  
Novel Method

DIRECT MEASUREMENT OF ARGININE-VASOPRESSIN IN HUMAN SERUM WITHOUT EXTRACTION PROCEDURE

1975 ◽  
Vol 80 (1_Suppla) ◽  
pp. S130 ◽  
Author(s):  
H. Wagner ◽  
V. Maier ◽  
H.-J. Herrmann ◽  
H. E. Franz
Keyword(s):  
Human Serum ◽  
Direct Measurement ◽  
Arginine Vasopressin ◽  
Extraction Procedure

scholarly journals Novel Method for the Direct Measurement of the τ Lepton Dipole Moments

2019 ◽  
Vol 123 (1) ◽  
Author(s):  
J. Fu ◽  
M. A. Giorgi ◽  
L. Henry ◽  
D. Marangotto ◽  
F. Martínez Vidal ◽  
...  
Keyword(s):  
Direct Measurement ◽  
Dipole Moments ◽  
Novel Method

A Radioreceptor Assay for Insulin: Direct Measurement of Human Serum Insulin

1982 ◽  
Vol 14 (07) ◽  
pp. 339-342 ◽  
Author(s):  
K. Nakao ◽  
A. Takeda ◽  
S. Kagawa ◽  
S. Shimizu ◽  
A. Matsuoka
Keyword(s):  
Human Serum ◽  
Direct Measurement ◽  
Serum Insulin ◽  
Radioreceptor Assay

A novel method for direct measurement of the pKa's of weakly acidic hydrocarbons

1978 ◽  
Vol 43 (9) ◽  
pp. 1812-1813 ◽  
Author(s):  
J. O. Stoffer ◽  
D. R. Strait ◽  
D. L. Filger ◽  
E. T. Lloyd ◽  
C. Crain
Keyword(s):  
Direct Measurement ◽  
Novel Method

A novel method for the direct measurement of urinary conjugated metabolites of α-tocopherol and its use in diabetes

2010 ◽  
Vol 54 (5) ◽  
pp. 599-600 ◽  
Author(s):  
Gayatri Sharma ◽  
David Muller ◽  
Stephen O'Riordan ◽  
Sinead Bryan ◽  
Peter Hindmarsh ◽  
...  
Keyword(s):  
Direct Measurement ◽  
Novel Method ◽  
Conjugated Metabolites

scholarly journals A Novel Method for Determination of Protein in Human Serum Utilizing the Trihydroxylphenylfluorone-Molybdenum(VI) Complex as a Probe by Fluorescence Spectrometry

2007 ◽  
Vol 90 (1) ◽  
pp. 238-243 ◽  
Author(s):  
Qin Wei ◽  
Hongmin Ma ◽  
Caihong Duan ◽  
Jin Wang ◽  
Shuyuan Liu ◽  
...  
Keyword(s):  
Aqueous Solution ◽  
Amino Acids ◽  
Detection Limit ◽  
Human Serum ◽  
Fluorescence Intensity ◽  
Acetate Buffer Solution ◽  
Buffer Solution ◽  
Protein Assay ◽  
Novel Method

Abstract The fluorescence intensity of the trihydroxylphenylfluorone-molybdenum(VI) Mo(VI) complex is quenched by protein. Based on this, a novel method for protein assay in aqueous solution was developed. With pH 3.75 acetic acidsodium acetate buffer solution, in the presence of p-octyl polyethylene glycol phenyl ether microemulsion, the quenched fluorescence intensity is proportional to the concentration of bovine serum albumin (BSA) in the range of 07.00 μg/mL, and the detection limit of BSA is 5.65 ng/mL. There is no interference from amino acids and most metal ions. The method developed in this paper has been used for the successful determination of protein in human serum.

Analysis of UK Sera for Aflatoxin by Enzyme-linked Immunosorbent Assay

1988 ◽  
Vol 7 (4) ◽  
pp. 353-356 ◽  
Author(s):  
A.P. Wilkinson ◽  
D.W. Denning ◽  
M.R.A. Morgan
Keyword(s):  
Human Serum ◽  
Direct Measurement ◽  
Immunosorbent Assay ◽  
Human Exposure ◽  
Blood Donors ◽  
Enzyme Linked Immunosorbent Assay ◽  
Fungal Metabolites ◽  
Serum Samples ◽  
Imported Food ◽  
The Uk

1 Aflatoxins are toxic, carcinogenic secondary fungal metabolites produced by certain moulds that commonly infest foods. Measurement of aflatoxins in human serum would give a direct measurement of exposure. 2 Twenty-seven serum samples from UK blood donors were found to contain aflatoxin levels not greater than 64 pmol/1 (20 pg/ml) by an enzyme-linked immunosorbent assay. 3 These findings may indicate that present UK guideline tolerances for aflatoxin in imported food are effective in limiting human exposure to toxic aflatoxins in the UK diet, though further work would be needed to confirm this. In particular, sub-populations suspected of being at higher risk may need special considerations.

scholarly journals An empirical approach to the extragalactic background light from AEGIS galaxy SED-type fractions

2011 ◽  
Vol 7 (S284) ◽  
pp. 442-445
Author(s):  
Alberto Domínguez
Keyword(s):  
Galaxy Evolution ◽  
Direct Measurement ◽  
Spectral Energy ◽  
Intensity Level ◽  
Energy Distributions ◽  
Extragalactic Background Light ◽  
Background Light ◽  
Luminosity Functions ◽  
Novel Method ◽  
Γ Ray

AbstractThe extragalactic background light (EBL) is of fundamental importance both for understanding the entire process of galaxy evolution and for γ-ray astronomy. However, the overall spectrum of the EBL between 0.1 and 1000 μm has never been determined directly, neither from observed luminosity functions (LFs), over a wide redshift range, nor from any multiwavelength observation of galaxy spectral energy distributions (SEDs). The evolving overall spectrum of the EBL is derived here utilizing a novel method based on observations only. It is emphasized that the local EBL seems already well constrained from the UV up to the mid-IR. Different independent methodologies such as direct measurement, galaxy counts, γ-ray attenuation and realistic EBL modelings point towards the same EBL intensity level. Therefore, a relevant contribution from Pop III stars to the local EBL seems unlikely.

scholarly journals Evaluation of the Novel Method and the Regression Equation for Calculation of Low-Density Lipoprotein Cholesterol

2015 ◽  
Vol 5 (1) ◽  
pp. 10-14 ◽  
Author(s):  
Muhammad Saiedullah ◽  
Nasreen Chowdhury ◽  
Md Aminul Haque Khan
Keyword(s):  
Direct Measurement ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Lipoprotein Cholesterol ◽  
Low Density ◽  
The Novel ◽  
Low Density Lipoprotein Cholesterol ◽  
Mean Values ◽  
Novel Method ◽  
Bangladeshi Population

Background: Friedewald’s formula (FF) is used worldwide to calculate low-density lipoprotein cholesterol (LDL-chol). But it has several shortcomings: overestimation at lower triglyceride (TG) concentrations and underestimation at higher concentrations. In FF, TG to very low-density lipoprotein cholesterol (VLDL-chol) ratio (TG/VLDL-chol) is considered as constant, but practically it is not a fixed value. Recently, by analyzing lipid profiles in a large population, continuously adjustable values of TG/VLDL-chol were used to derive a novel method (NM) for the calculation of LDL-chol. Objective: The aim of this study was to evaluate the performance of the novel method compared with direct measurement and regression equation (RE) developed for Bangladeshi population. Materials and Methods: In this cross-sectional comparative study we used lipid profiles of 955 adult Bangladeshi subjects. Total cholesterol (TC), TG, HDL-chol and LDL-chol were measured by direct methods using automation. LDL-chol was also calculated by NM and RE. LDL-chol calculated by NM and RE were compared with measured LDL-chol by twotailed paired t test, Pearson’s correlation test, bias against measured LDL-chol by Bland-Altman test, accuracy within ±5% and ±12% of measured LDL-chol and by inter-rater agreements with measured LDL-chol at different cut-off values. Results: The mean values of LDL-chol were 110.7 ± 32.0 mg/dL for direct measurement, 111.9 ± 34.8 mg/dL for NM and 113.2 ± 31.7 mg/dL for RE. Mean values of calculated LDL-chol by both NM and RE differed from that of measured LDL-chol (p<0.01 for NM and p<0.0001 for RE). The correlation coefficients of calculated LDL-chol values with measured LDL-chol were 0.944 (p<0.0001) for NM and 0.945 (p<0.0001) for RE. Bland- Altman plots showed good agreement between calculated and measured LDL-chol. Accuracy within ±5% of measured LDL-chol was 49% for NM, 46% for RE and within ±12% of measured LDL-chol was 79% for both NM and RE. Inter-rater agreements (?) between calculated and measured LDL-chol at LDL-chol <100 mg/dL, 100–130 mg/dL and >130 mg/dL were 0.816 vs 0.815, 0.637 vs 0.649 and 0.791 vs 0.791 for NM and RE respectively. Conclusion: This study reveals that NM and RE developed for Bangladeshi population have similar performance and can be used for the calculation of LDL-chol. DOI: http://dx.doi.org/10.3329/jemc.v5i1.21491 J Enam Med Col 2015; 5(1): 10-14

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