scholarly journals Genetic Identification ofStaphylococcus aureusby Polymerase Chain Reaction Using Single-Base-Pair Mismatch in 16S Ribosomal RNA Gene

1995 ◽  
Vol 39 (11) ◽  
pp. 839-844 ◽  
Author(s):  
Katsutoshi Saruta ◽  
Sadayori Hoshina ◽  
Katsuhiko Machida
Keyword(s):  
Polymerase Chain Reaction ◽  
Ribosomal Rna ◽  
Base Pair ◽  
Genetic Identification ◽  
Chain Reaction ◽  
Single Base ◽  
Base Pair Mismatch ◽  
16S Ribosomal Rna Gene ◽  
Single Base Pair ◽  
Polymerase Chain

New Somatic Mutation in the PIG-A Gene Emerges at Relapse of Paroxysmal Nocturnal Hemoglobinuria

Blood ◽  
1998 ◽  
Vol 92 (9) ◽  
pp. 3422-3427 ◽  
Author(s):  
Khédoudja Nafa ◽  
Monica Bessler ◽  
H. Joachim Deeg ◽  
Lucio Luzzatto
Keyword(s):  
Bone Marrow ◽  
Polymerase Chain Reaction ◽  
Longitudinal Study ◽  
Paroxysmal Nocturnal Hemoglobinuria ◽  
Pcr Amplification ◽  
Chain Reaction ◽  
Single Base ◽  
Single Base Pair ◽  
Polymerase Chain ◽  
American Society

We report a detailed longitudinal study of the first patient to be treated (in 1973) for paroxysmal nocturnal hemoglobinuria (PNH) with syngeneic bone marrow transplantation (BMT). The patient subsequently relapsed with PNH in 1983, and still has PNH to date. Analysis of thePIG-A gene in a recent blood sample showed in exon 6 an insertion-duplication causing a frameshift. Polymerase chain reaction (PCR) amplification of the PIG-A exon 6 from bone marrow (BM) slides obtained before BMT showed that the duplication was not present; instead, we found several single base pair substitutions in exons 2 and 6. Thus, relapse of PNH in this patient was not due to persistence of the original clones; rather, it was associated with the emergence of a new clone. These findings support the notion that the BM environment may create selective conditions favoring the expansion of PNH clones.© 1998 by The American Society of Hematology.

New Somatic Mutation in the PIG-A Gene Emerges at Relapse of Paroxysmal Nocturnal Hemoglobinuria

Blood ◽  
1998 ◽  
Vol 92 (9) ◽  
pp. 3422-3427 ◽  
Author(s):  
Khédoudja Nafa ◽  
Monica Bessler ◽  
H. Joachim Deeg ◽  
Lucio Luzzatto
Keyword(s):  
Bone Marrow ◽  
Polymerase Chain Reaction ◽  
Longitudinal Study ◽  
Paroxysmal Nocturnal Hemoglobinuria ◽  
Pcr Amplification ◽  
Chain Reaction ◽  
Single Base ◽  
Single Base Pair ◽  
Polymerase Chain ◽  
American Society

Abstract We report a detailed longitudinal study of the first patient to be treated (in 1973) for paroxysmal nocturnal hemoglobinuria (PNH) with syngeneic bone marrow transplantation (BMT). The patient subsequently relapsed with PNH in 1983, and still has PNH to date. Analysis of thePIG-A gene in a recent blood sample showed in exon 6 an insertion-duplication causing a frameshift. Polymerase chain reaction (PCR) amplification of the PIG-A exon 6 from bone marrow (BM) slides obtained before BMT showed that the duplication was not present; instead, we found several single base pair substitutions in exons 2 and 6. Thus, relapse of PNH in this patient was not due to persistence of the original clones; rather, it was associated with the emergence of a new clone. These findings support the notion that the BM environment may create selective conditions favoring the expansion of PNH clones. © 1998 by The American Society of Hematology.

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