Development of a Colorimetric, Second Generation Nucleic Acid Hybridization Method For Detection of Salmonella in Foods and a Comparison With Conventional Culture Procedure

1990 ◽  
Vol 55 (5) ◽  
pp. 1394-1398 ◽  
Author(s):  
S.G. WILSON ◽  
S. CHAN ◽  
M. DEROO ◽  
M. VERA-GARCIA ◽  
A. JOHNSON ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Second Generation ◽  
Nucleic Acid Hybridization ◽  
Hybridization Method ◽  
Conventional Culture ◽  
Culture Procedure

Comparative Study of Colorimetric DNA Hybridization Method and Conventional Culture Procedure for Detection of Salmonella in Foods

1990 ◽  
Vol 73 (3) ◽  
pp. 419-424 ◽  
Author(s):  
Samuel W Chan ◽  
Stephen G Wilson ◽  
Marcela Vera-Garcia ◽  
Kevan Whippie ◽  
Margaret Ottaviani ◽  
...  
Keyword(s):  
Dna Hybridization ◽  
Culture Method ◽  
Nucleic Acid Hybridization ◽  
Hybridization Method ◽  
Conventional Culture ◽  
End Point Detection ◽  
Salmonella Serotypes ◽  
Point Detection ◽  
Conventional Culture Method ◽  
Culture Procedure

Abstract A second generation nucleic acid hybridization assay has been developed and evaluated against the conventional culture method for detection of salmonellae In foods. The assay Involves a liquid hybridization with Sa/mone/fo-speclflc oligonucleotide probes, capture of probe:target hybrids onto a solid support (plastic dipstick), and a colorimetric end point detection. The assay can be completed In 2.5 h, following approximately 44 h of culture enrichment. One thousand samples representing 20 food types were analyzed in parallel by both methods. Samples Included unlnoculated test product, and product Inoculated with Salmonella at 2 levels. Eighteen Salmonella serotypes were used as Inocula. The data demonstrate that the colorlmetric hybridization method and the conventional culture method are equivalent In their ability to detect Salmonella contamination of foods.

Comparative Studies of Nucleic Acid Hybridization Assay for Listeria in Foods

1988 ◽  
Vol 71 (3) ◽  
pp. 669-673 ◽  
Author(s):  
Jeffrey D Klinger ◽  
Andrew Johnson ◽  
Daniel Croan ◽  
Pauline Flynn ◽  
Kevan Whippie ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Environmental Samples ◽  
False Negative ◽  
False Negative Rate ◽  
Nucleic Acid Hybridization ◽  
Hybridization Assay ◽  
Rapid Screening ◽  
Bacterial Strains ◽  
Synthetic Dna ◽  
Conventional Culture

Abstract A nucleic acid hybridization assay has been developed for Listeria spp. in dairy foods and environmental samples. The assay is based on detection of unique Listeria 16S rRNA sequences by using a 32Plabeled synthetic DNA probe. Inclusivity and exclusivity of the probe were confirmed with 139 Listeria isolates representing all known species, and 73 non-Listeria bacterial strains. In this paper, we present results from our preliminary studies comparing the hybridization assay with conventional culture on a total of 575 specimens that represent a variety of inoculated and uninoculated foods and environmental samples. The assay, which is done in a filter manifold format after 2 days of cultural enrichment, requires a total assay time of less than 2.5 days. The false-negative rate for all sample groups tested using the GENE-TRAK hybridization assay was less than the rate for culture. Thus, the new assay allows rapid screening of the indicated product groups and provides reliable numerical results

Comparative Study of a DNA Hybridization Method and the Conventional Culture Procedure for Detection ofSalmonellain Foods

1987 ◽  
Vol 52 (3) ◽  
pp. 781-785 ◽  
Author(s):  
R. S. FLOWERS ◽  
M. A. MOZOLA ◽  
M. S. CURIALE ◽  
D. A. GABIS ◽  
J. H. SILLIKER
Keyword(s):  
Comparative Study ◽  
Dna Hybridization ◽  
Hybridization Method ◽  
Conventional Culture ◽  
Culture Procedure

scholarly journals Optimization of excimer-forming two-probe nucleic acid hybridization method with pyrene as a fluorophore

1998 ◽  
Vol 26 (23) ◽  
pp. 5409-5416 ◽  
Author(s):  
M. Masuko ◽  
H. Ohtani ◽  
K. Ebata ◽  
A. Shimadzu
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acid Hybridization ◽  
Hybridization Method

Hierarchical Assembly of Viral Nanotemplates with Encoded Microparticles via Nucleic Acid Hybridization

Langmuir ◽  
2008 ◽  
Vol 24 (21) ◽  
pp. 12483-12488 ◽  
Author(s):  
Wui Siew Tan ◽  
Christina L. Lewis ◽  
Nicholas E. Horelik ◽  
Daniel C. Pregibon ◽  
Patrick S. Doyle ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acid Hybridization ◽  
Hierarchical Assembly
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