Note:Development of an external quality assurance scheme for the detection and enumeration of Pseudomonas aeruginosa from water and comparison of results using modified King's A broth and a commercial agar

1996 ◽  
Vol 80 (6) ◽  
pp. 682-686 ◽  
Author(s):  
B.M. Place ◽  
H.E. Tillett ◽  
N.F. Lightfoot
Author(s):  
P. Ruiz Sala ◽  
G. Ruijter ◽  
C. Acquaviva ◽  
A. Chabli ◽  
M. G. M. de Sain-van der Velden ◽  
...  

1997 ◽  
Vol 35 (11-12) ◽  
pp. 471-473
Author(s):  
J. Sellwood ◽  
J. Shore

The Water Virology External Quality Assurance (EQA) Scheme has distributed samples containing enterovirus to the specialised virus laboratories in Britain which analyse recreational water. Duplicate samples were either assayed for direct virus count or added to 101 of water, concentrated to 10ml and then assayed for virus counts. Although similar counts were obtained in different laboratories on direct assays, a wide range of virus counts was reported after water processing. However, robust attention to method detail and stringent quality control has been shown to improve the consistency of virus recovery rates. The results of tests for enterovirus in recreational water can then be used with confidence.


Urolithiasis ◽  
2020 ◽  
Vol 48 (6) ◽  
pp. 473-480
Author(s):  
Felicity Stokes ◽  
Cecile Acquaviva-Bourdain ◽  
Bernd Hoppe ◽  
John C. Lieske ◽  
Elisabeth Lindner ◽  
...  

AbstractMeasurement of oxalate in the blood is essential for monitoring primary hyperoxaluria patients with progressive renal impairment and on dialysis prior to transplantation. As no external quality assurance scheme is available for this analyte, we conducted a sample exchange scheme between six laboratories specifically involved with the investigation of primary hyperoxaluria to compare results. The methodologies compared were gas chromatography/mass spectrometry (GCMS), ion chromatography with mass spectrometry (ICMS), and enzymatic methods using oxalate oxidase and spectrophotometry. Although individual laboratories performed well in terms of reproducibility and linearity, there was poor agreement (absolute values) between centres as illustrated by a longer-term comparison of patient results from two of the participating laboratories. This situation was only partly related to differences in calibration and mainly reflected the lower recoveries seen with the ultrafiltration of samples. These findings lead us to conclude that longitudinal monitoring of primary hyperoxaluria patients with deteriorating kidney function should be performed by a single consistent laboratory and the methodology used should always be defined. In addition, plasma oxalate concentrations reported in registry studies and those associated with the risk of systemic oxalosis in published studies need to be interpreted in light of the methodology used. A reference method and external quality assurance scheme for plasma oxalate analysis would be beneficial.


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