Close genetic linkage of genes conferring adult-plant resistance to leaf rust and stripe rust in wheat

Seedling resistances to stem rust, leaf rust and stripe rust were evaluated in the 37th International Triticale Screening Nursery, distributed by the International Wheat and Maize Improvement Centre (CIMMYT) in 2005. In stem rust tests, 12 and 69 of a total of 81 entries were postulated to carry Sr27 and SrSatu, respectively. When compared with previous studies of CIMMYT triticale nurseries distributed from 1980 to 1986 and 1991 to 1993, the results suggest a lack of expansion in the diversity of stem rust resistance. A total of 62 of 64 entries were resistant to five leaf rust pathotypes. In stripe rust tests, ~93% of the lines were postulated to carry Yr9 alone or in combination with other genes. The absence of Lr26 in these entries indicated that Yr9 and Lr26 are not genetically associated in triticale. A high proportion of nursery entries (63%) were postulated to carry an uncharacterised gene, YrJackie. The 13 lines resistant to stripe rust and the 62 entries resistant to leaf rust represent potentially useful sources of seedling resistance in developing new triticale cultivars. Field rust tests are needed to verify if seedling susceptible entries also carry adult plant resistance.

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Genetic Association of Leaf Rust Resistance GeneLr34with Adult Plant Resistance to Stripe Rust in Bread Wheat

Phytopathology ◽

10.1094/phyto-82-835 ◽

1992 ◽

Vol 82 (8) ◽

pp. 835 ◽

Cited By ~ 165

Author(s):

R. P. Singh

Keyword(s):

Leaf Rust ◽

Genetic Association ◽

Bread Wheat ◽

Stripe Rust ◽

Plant Resistance ◽

Rust Resistance ◽

Adult Plant Resistance ◽

Leaf Rust Resistance ◽

Adult Plant

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An introgression on wheat chromosome 4DL in RL6077 (Thatcher*6/PI 250413) confers adult plant resistance to stripe rust and leaf rust (Lr67)

Theoretical and Applied Genetics ◽

10.1007/s00122-010-1373-y ◽

2010 ◽

Vol 121 (6) ◽

pp. 1083-1091 ◽

Cited By ~ 87

Author(s):

Colin W. Hiebert ◽

Julian B. Thomas ◽

Brent D. McCallum ◽

D. Gavin Humphreys ◽

Ronald M. DePauw ◽

...

Keyword(s):

Leaf Rust ◽

Stripe Rust ◽

Plant Resistance ◽

Adult Plant Resistance ◽

Wheat Chromosome ◽

Adult Plant

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Characterization of Adult Plant Resistance to Leaf Rust and Stripe Rust in Indian Wheat Cultivar ‘New Pusa 876’

Crop Science ◽

10.2135/cropsci2017.06.0396 ◽

2018 ◽

Vol 58 (2) ◽

pp. 630-638 ◽

Cited By ~ 8

Author(s):

Luis J. Ponce‐Molina ◽

Julio Huerta‐Espino ◽

Ravi P. Singh ◽

Bhoja R. Basnet ◽

Evans Lagudah ◽

...

Keyword(s):

Leaf Rust ◽

Stripe Rust ◽

Plant Resistance ◽

Adult Plant Resistance ◽

Wheat Cultivar ◽

Adult Plant

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Identification and Mapping of Adult Plant Resistance Loci to Leaf Rust and Stripe Rust in Common Wheat Cultivar Kundan

Plant Disease ◽

10.1094/pdis-06-16-0890-re ◽

2017 ◽

Vol 101 (3) ◽

pp. 456-463 ◽

Cited By ~ 14

Author(s):

Y. Ren ◽

R. P. Singh ◽

B. R. Basnet ◽

C. X. Lan ◽

J. Huerta-Espino ◽

...

Keyword(s):

Leaf Rust ◽

Stripe Rust ◽

Plant Resistance ◽

Adult Plant Resistance ◽

Average Distance ◽

Recombinant Inbred Lines ◽

Interval Mapping ◽

Snp Markers ◽

Adult Plant ◽

Additive Interaction

Leaf rust (LR) and stripe rust (YR) are important diseases of wheat worldwide. We used 148 recombinant inbred lines (RIL) from the cross of Avocet × Kundan for determining and mapping the genetic basis of adult plant resistance (APR) loci. The population was phenotyped LR and YR for three seasons in field trials conducted in Mexico and genotyped with the diversity arrays technology sequencing (DArT-Seq) and simple sequence repeat markers. The final genetic map was constructed using 2,937 polymorphic markers with an average distance of 1.29 centimorgans between markers. Inclusive composite interval mapping identified two co-located APR quantitative trait loci (QTL) for LR and YR, two LR QTL, and three YR QTL. The co-located resistance QTL on chromosome 1BL corresponded to the pleiotropic APR gene Lr46/Yr29. QLr.cim-2BL, QYr.cim-2AL, and QYr.cim-5AS could be identified as new resistance loci in this population. Lr46/Yr29 contributed 49.5 to 65.1 and 49.2 to 66.1% of LR and YR variations, respectively. The additive interaction between detected QTL showed that LR severities for RIL combining four QTL ranged between 5.3 and 25.8%, whereas the lowest YR severities were for RIL carrying QTL on chromosomes 1BL + 2AL + 6AL. The high-density DArT-Seq markers across chromosomes can be used in fine mapping of the targeted loci and development SNP markers.

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Characterization of genetic loci conferring adult plant resistance to leaf rust and stripe rust in spring wheat

Genome ◽

10.1139/g06-052 ◽

2006 ◽

Vol 49 (8) ◽

pp. 977-990 ◽

Cited By ~ 110

Author(s):

H M William ◽

R P Singh ◽

J Huerta-Espino ◽

G Palacios ◽

K Suenaga

Keyword(s):

Leaf Rust ◽

Stripe Rust ◽

Plant Resistance ◽

Adult Plant Resistance ◽

Puccinia Striiformis ◽

Bulked Segregant Analysis ◽

Puccinia Triticina ◽

Adult Plant ◽

Single Chromosome ◽

Slow Rusting

Leaf (brown) and stripe (yellow) rusts, caused by Puccinia triticina and Puccinia striiformis, respectively, are fungal diseases of wheat (Triticum aestivum) that cause significant yield losses annually in many wheat-growing regions of the world. The objectives of our study were to characterize genetic loci associated with resistance to leaf and stripe rusts using molecular markers in a population derived from a cross between the rust-susceptible cultivar 'Avocet S' and the resistant cultivar 'Pavon76'. Using bulked segregant analysis and partial linkage mapping with AFLPs, SSRs and RFLPs, we identified 6 independent loci that contributed to slow rusting or adult plant resistance (APR) to the 2 rust diseases. Using marker information available from existing linkage maps, we have identified additional markers associated with resistance to these 2 diseases and established several linkage groups in the 'Avocet S' × 'Pavon76' population. The putative loci identified on chromosomes 1BL, 4BL, and 6AL influenced resistance to both stripe and leaf rust. The loci on chromosomes 3BS and 6BL had significant effects only on stripe rust, whereas another locus, characterized by AFLP markers, had minor effects on leaf rust only. Data derived from Interval mapping indicated that the loci identified explained 53% of the total phenotypic variation (R2) for stripe rust and 57% for leaf rust averaged across 3 sets of field data. A single chromosome recombinant line population segregating for chromosome 1B was used to map Lr46/Yr29 as a single Mendelian locus. Characterization of slow-rusting genes for leaf and stripe rust in improved wheat germplasm would enable wheat breeders to combine these additional loci with known slow-rusting loci to generate wheat cultivars with higher levels of slow-rusting resistance.Key words: Puccinia triticina, Puccinia striiformis, Triticum aestivum, bulked segregant analysis, single chromosome recombinant lines, linkage mapping, adult plant resistance.

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QTL mapping of adult plant resistance to stripe rust and leaf rust in a Fuyu 3/Zhengzhou 5389 wheat population

The Crop Journal ◽

10.1016/j.cj.2019.09.013 ◽

2020 ◽

Vol 8 (4) ◽

pp. 655-665 ◽

Cited By ~ 2

Author(s):

Takele Weldu Gebrewahid ◽

Peipei Zhang ◽

Yue Zhou ◽

Xiaocui Yan ◽

Xianchun Xia ◽

...

Keyword(s):

Qtl Mapping ◽

Leaf Rust ◽

Stripe Rust ◽

Plant Resistance ◽

Adult Plant Resistance ◽

Adult Plant ◽

Wheat Population

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Molecular Marker Mapping of Leaf Rust Resistance Gene Lr46 and Its Association with Stripe Rust Resistance Gene Yr29 in Wheat

Phytopathology ◽

10.1094/phyto.2003.93.2.153 ◽

2003 ◽

Vol 93 (2) ◽

pp. 153-159 ◽

Cited By ~ 159

Author(s):

M. William ◽

R. P. Singh ◽

J. Huerta-Espino ◽

S. Ortiz Islas ◽

D. Hoisington

Keyword(s):

Leaf Rust ◽

Resistance Gene ◽

Stripe Rust ◽

Plant Resistance ◽

Rust Resistance ◽

Adult Plant Resistance ◽

Wheat Chromosome ◽

Rust Resistance Gene ◽

Adult Plant ◽

Genomic Location

Leaf and stripe rusts, caused by Puccinia triticina and P. striiformis, respectively, are globally important fungal diseases of wheat that cause significant annual yield losses. A gene that confers slow rusting resistance to leaf rust, designated as Lr46, has recently been located on wheat chromosome 1B. The objectives of our study were to establish the precise genomic location of gene Lr46 using molecular approaches and to determine if there was an association of this locus with adult plant resistance to stripe rust. A population of 146 F5 and F6 lines produced from the cross of susceptible ‘Avocet S’ with resistant ‘Pavon 76’ was developed and classified for leaf rust and stripe rust severity for three seasons. Using patterns of segregation for the two diseases, we estimated that at least two genes with additive effects conferred resistance to leaf rust and three to four genes conferred resistance to stripe rust. Bulked segregant analysis and linkage mapping using amplified fragment length polymorphisms with the ‘Avocet’ × ‘Pavon 76’ population, F3 progeny lines of a single chromosome recombinant line population from the cross ‘Lalbahadur’ × ‘Lalbahadur (Pavon 1B)’, and the International Triticeae Mapping Initiative population established the genomic location of Lr46 at the distal end of the long arm of wheat chromosome 1B. A gene that is closely linked to Lr46 and confers moderate levels of adult plant resistance to stripe rust is identified and designated as Yr29.

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