scholarly journals Expression of three human beta-adrenergic-receptor subtypes in transfected chinese hamster ovary cells

1991 ◽  
Vol 196 (2) ◽  
pp. 357-361 ◽  
Author(s):  
Keri M. TATE ◽  
Marie-M. BRIEND-SUTREN ◽  
Laurent J. EMORINE ◽  
Colette DELAVIER-KLUTCHKO ◽  
Stefano MARULLO ◽  
...  
Keyword(s):  
Chinese Hamster Ovary ◽  
Adrenergic Receptor ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Receptor Subtypes ◽  
Beta Adrenergic Receptor ◽  
Beta Adrenergic ◽  
Ovary Cells

scholarly journals Recombinant bovine neurokinin-2 receptor stably expressed in Chinese hamster ovary cells couples to multiple signal transduction pathways.

1991 ◽  
Vol 2 (10) ◽  
pp. 767-779 ◽  
Author(s):  
H R Eistetter ◽  
D J Church ◽  
A Mills ◽  
P P Godfrey ◽  
A M Capponi ◽  
...  
Keyword(s):  
Signal Transduction ◽  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Secondary Response ◽  
Signal Transduction Pathways ◽  
Receptor Subtypes ◽  
Neurokinin A ◽  
Ovary Cells ◽  
Multiple Signal

Neurokinins are a family of neuropeptides with widespread distribution mediating a broad spectrum of physiological actions through three distinct receptor subtypes: NK-1, NK-2, and NK-3. We investigated some of the second messenger and cellular processes under control by the recombinant bovine NK-2 receptor stably expressed in Chinese hamster ovary cells. In this system the NK-2 receptor displays its expected pharmacological characteristics, and the physiological agonist neurokinin A stimulates several cellular responses. These include 1) transient inositol 1,4,5-trisphosphate (IP3) formation and Ca2+ mobilization, 2) increased out put of arachidonic acid and prostaglandin E2 (PGE2), 3) enhanced cyclic AMP (cAMP) generation, 4) increased de novo DNA synthesis, and 5) an induction of the "immediate early" genes c-fos and c-jun. Although NK-2 receptor-mediated IP3 formation involves activation of a pertussis toxin-insensitive G-protein, increased cAMP production is largely a secondary response and can be at least partially attributed to autocrine stimulation by endogenously generated eicosanoids, particularly PGE2. This is the first demonstration that a single recombinant neurokinin receptor subtype can regulate, either directly or indirectly, multiple signal transduction pathways and suggests several potential important mediators of neurokinin actions under physiological conditions.

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