Atypical Porcine Type I Interferon. Biochemical and Biological Characterization of the Recombinant Protein Expressed in Insect Cells

1995 ◽  
Vol 230 (1) ◽  
pp. 200-206 ◽  
Author(s):  
Ping-De Niu ◽  
Francois Lefevre ◽  
Dominique Mege ◽  
Claude Bonnardiere
Keyword(s):  
Recombinant Protein ◽  
Insect Cells ◽  
Type I Interferon ◽  
Type I ◽  
Biological Characterization

PS2-60 Biological characterization of feline interferon-NU: a new member of the Type I interferon family

Cytokine ◽  
2010 ◽  
Vol 52 (1-2) ◽  
pp. 63
Author(s):  
Steven Carbone ◽  
Sidney Pestka ◽  
Ronald G. Jubin
Keyword(s):  
Type I Interferon ◽  
Type I ◽  
Biological Characterization

Molecular Characterization of Feline Type I Interferon Receptor 2

2010 ◽  
Vol 30 (2) ◽  
pp. 81-88 ◽  
Author(s):  
Qinghua Xue ◽  
Limin Yang ◽  
Xiaoling Liu ◽  
Wenjun Liu
Keyword(s):  
Molecular Characterization ◽  
Type I Interferon ◽  
Type I ◽  
Interferon Receptor

361 Characterization of the bovine Type I interferon locus

Cytokine ◽  
2008 ◽  
Vol 43 (3) ◽  
pp. 329
Author(s):  
Angela Walker ◽  
R. Michael Roberts
Keyword(s):  
Type I Interferon ◽  
Type I ◽  
Bovine Type

Molecular cloning and functional characterization of two duplicated two-cysteine containing type I interferon genes in rock bream Oplegnathus fasciatus

2012 ◽  
Vol 33 (4) ◽  
pp. 886-898 ◽  
Author(s):  
Qiang Wan ◽  
W.D. Niroshana Wicramaarachchi ◽  
Ilson Whang ◽  
Bong-Soo Lim ◽  
Myung-Joo Oh ◽  
...  
Keyword(s):  
Molecular Cloning ◽  
Type I Interferon ◽  
Functional Characterization ◽  
Type I ◽  
Oplegnathus Fasciatus ◽  
Rock Bream

Purification and biological characterization of soluble, recombinant mouse IFNβ expressed in insect cells

2014 ◽  
Vol 94 ◽  
pp. 7-14 ◽  
Author(s):  
Sebastian A. Stifter ◽  
Jodee A. Gould ◽  
Niamh E. Mangan ◽  
Hugh H. Reid ◽  
Jamie Rossjohn ◽  
...  
Keyword(s):  
Insect Cells ◽  
Biological Characterization ◽  
Recombinant Mouse

scholarly journals Mammalian Casein Kinase 1α and Its Leishmanial Ortholog Regulate Stability of IFNAR1 and Type I Interferon Signaling

2009 ◽  
Vol 29 (24) ◽  
pp. 6401-6412 ◽  
Author(s):  
Jianghuai Liu ◽  
Lucas P. Carvalho ◽  
Sabyasachi Bhattacharya ◽  
Christopher J. Carbone ◽  
K. G. Suresh Kumar ◽  
...  
Keyword(s):  
Er Stress ◽  
Mammalian Cells ◽  
Leishmania Major ◽  
Type I Interferon ◽  
Casein Kinase ◽  
Type I ◽  
Bona Fide ◽  
Activity Dependent

ABSTRACT Phosphorylation of the degron of the IFNAR1 chain of the type I interferon (IFN) receptor triggers ubiquitination and degradation of this receptor and, therefore, plays a crucial role in negative regulation of IFN-α/β signaling. Besides the IFN-stimulated and Jak activity-dependent pathways, a basal ligand-independent phosphorylation of IFNAR1 has been described and implicated in downregulating IFNAR1 in response to virus-induced endoplasmic reticulum (ER) stress. Here we report purification and characterization of casein kinase 1α (CK1α) as a bona fide major IFNAR1 kinase that confers basal turnover of IFNAR1 and cooperates with ER stress stimuli to mediate phosphorylation-dependent degradation of IFNAR1. Activity of CK1α was required for phosphorylation and downregulation of IFNAR1 in response to ER stress and viral infection. While many forms of CK1 were capable of phosphorylating IFNAR1 in vitro, human CK1α and L-CK1 produced by the protozoan Leishmania major were also capable of increasing IFNAR1 degron phosphorylation in cells. Expression of leishmania CK1 in mammalian cells stimulated the phosphorylation-dependent downregulation of IFNAR1 and attenuated its signaling. Infection of mammalian cells with L. major modestly decreased IFNAR1 levels and attenuated cellular responses to IFN-α in vitro. We propose a role for mammalian and parasite CK1 enzymes in regulating IFNAR1 stability and type I IFN signaling.

PS1-025. Characterization of a novel type I Interferon, IFNε

Cytokine ◽  
2011 ◽  
Vol 56 (1) ◽  
pp. 23
Author(s):  
Sebastian A. Stifter ◽  
Kayee Fung ◽  
Niamh Mangan ◽  
Nicole de Weerd ◽  
Paul Hertzog
Keyword(s):  
Type I Interferon ◽  
Type I

scholarly journals Cloning and Characterization of Soluble and Transmembrane Isoforms of a Novel Component of the Murine Type I Interferon Receptor, IFNAR 2

1997 ◽  
Vol 272 (38) ◽  
pp. 23865-23870 ◽  
Author(s):  
Catherine M. Owczarek ◽  
Seung Y. Hwang ◽  
Kerry A. Holland ◽  
Lerna M. Gulluyan ◽  
Michael Tavaria ◽  
...  
Keyword(s):  
Type I Interferon ◽  
Type I ◽  
Interferon Receptor

Analyses of genetic abnormalities in type I CD36 deficiency in Japan: identification and cell biological characterization of two novel mutations that cause CD36 deficiency in man

2001 ◽  
Vol 108 (6) ◽  
pp. 459-466 ◽  
Author(s):  
Hirokazu Kashiwagi ◽  
Yoshiaki Tomiyama ◽  
Shuichi Nozaki ◽  
Teruo Kiyoi ◽  
Seiji Tadokoro ◽  
...  
Keyword(s):  
Type I ◽  
Biological Characterization ◽  
Novel Mutations ◽  
Cd36 Deficiency ◽  
Genetic Abnormalities
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