Indirect Immunoperoxidase and Immunofluorescence Staining of Bordetella Bronchiseptica in Formalin-Fixed, Paraffin-Embedded Lung Tissues of Rabbits

1990 ◽  
Vol 37 (1-10) ◽  
pp. 1-7 ◽  
Author(s):  
F. A. Uzal ◽  
E. Rivera ◽  
R. E. Feinstein
Keyword(s):  
Bordetella Bronchiseptica ◽  
Immunofluorescence Staining ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Indirect Immunoperoxidase ◽  
Formalin Fixed

scholarly journals Detection of Ehrlichia Risticii using an Avidin-Biotin-Immunoperoxidase Staining System

1989 ◽  
Vol 1 (3) ◽  
pp. 215-218 ◽  
Author(s):  
Mike A. Breider ◽  
Ginger Callahan ◽  
Richard E. Corstvet
Keyword(s):  
Enzymatic Digestion ◽  
Staining Procedure ◽  
Immunoperoxidase Staining ◽  
Formalin Fixed Paraffin ◽  
Ehrlichia Risticii ◽  
Formalin Fixed Paraffin Embedded ◽  
Indirect Immunoperoxidase ◽  
Neutral Formalin ◽  
Positive Results ◽  
Formalin Fixed

An indirect immunoperoxidase procedure using a specific anti- Ehrlichia risticii monoclonal antibody and an avidin-biotin-peroxidase staining method was used to detect E. risticii antigen in infected P388D1 murine monocytes. Several different methods of cytological fixation were used, including acetone (15 min), 95% ethanol (15 min), Bouin's fixative (5 hr), and 10% buffered neutral formalin (24 hr). The E. risticii organisms were labeled effectively and identified in cells fixed with acetone and ethanol. However, infected P388D1 cells fixed in 10% formalin or Bouin's fixative required enzymatic digestion with 1.0% trypsin for 15 min at 37 C before positive results were evident. This indirect immunoperoxidase avidin-biotin staining procedure proved to be a sensitive assay for the detection of intracellular E. risticii and may be an effective diagnostic procedure for formalin-fixed paraffin-embedded tissue.

Proteome Analysis of Formalin-Fixed Paraffin-Embedded Tissues from a Primary Gastric Melanoma and its Meningeal Metastasis: A Case Report

2014 ◽  
Vol 14 (3) ◽  
pp. 382-387 ◽  
Author(s):  
Juliana Fischer ◽  
Nathalie Canedo ◽  
Katia Goncalves ◽  
Leila Chimelli ◽  
Monique Franca ◽  
...  
Keyword(s):  
Case Report ◽  
Proteome Analysis ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Formalin Fixed
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