High Activities of Glutamine Transaminase K (Dichlorovinylcysteine ?-Lyase) and ?-Amidase in the Choroid Plexus of Rat Brain

1993 ◽  
Vol 61 (5) ◽  
pp. 1731-1741 ◽  
Author(s):  
Arthur J. L. Cooper ◽  
Dicky G. Abraham ◽  
Alan S. Gelbard ◽  
James C. K. Lai ◽  
Carol K. Petito
Keyword(s):  
Author(s):  
Ana R. Costa-Brito ◽  
Telma Quintela ◽  
Isabel Gonçalves ◽  
Ana C. Duarte ◽  
Ana R. Costa ◽  
...  

1986 ◽  
Vol 34 (7) ◽  
pp. 949-952 ◽  
Author(s):  
A J Stauder ◽  
P W Dickson ◽  
A R Aldred ◽  
G Schreiber ◽  
F A Mendelsohn ◽  
...  

The sites of synthesis of transthyretin in the brain were investigated using in situ hybridization with [35S]-labeled recombinant cDNA probes specific for transthyretin mRNA. Autoradiography of hybridized coronal sections of rat brain revealed specific cellular localization of transthyretin mRNA in choroid plexus epithelial cells of the lateral, third, and fourth ventricles. Transferrin mRNA was also investigated and, in contrast to transthyretin mRNA, was localized mainly in the lateral ventricles. Our results indicate that substantial synthesis of transthyretin and transferrin mRNA may occur in the choroid plexus.


1987 ◽  
Vol 60 (719) ◽  
pp. 1109-1117 ◽  
Author(s):  
W. Calvo ◽  
J. W. Hopewell ◽  
H. S. Reinhold ◽  
A. P. van den Berg ◽  
T. K. Yeung

1994 ◽  
Vol 3 (3) ◽  
pp. 177-180 ◽  
Author(s):  
K. G. Go ◽  
J. G. Ter Haar ◽  
L. de Ley ◽  
F. Zuiderveen ◽  
L. Parente ◽  
...  

Lipocortin-1, lipocortin-2 and lipocortin-5 were immunohistochemically assessed in rats. Apart from animals receiving no treatment, other animals received pretreatment with methylprednisolone, or the 21-aminosteroid U-74389F. Whereas Hpocortin immunoreactivity was absent in the greater part of the brain in animals not pretreated with steroid (except in sporadic microglial cells and choroid plexus), there was obvious immunostaining of parenchymatous elements in steroid pretreated animals. In the steroid pretreated animals lipocortin immunoreactivity of the brain tissue may indicate local formation of lipocortin under the influence of steroids that had entered the tissue. The cellular elements which showed immunostaining included meningeal cells, neurones, ependyma, oligodendroglia and capillary endotheHum.


1987 ◽  
Vol 18 (4) ◽  
pp. 573-576 ◽  
Author(s):  
B. Bloch ◽  
T. Popovici ◽  
S. Chouham ◽  
M.J. Levin ◽  
D. Tuil ◽  
...  

Diabetes ◽  
1986 ◽  
Vol 35 (2) ◽  
pp. 246-249 ◽  
Author(s):  
D. G. Baskin ◽  
B. Brewitt ◽  
D. A. Davidson ◽  
E. Corp ◽  
T. Paquette ◽  
...  

Author(s):  
John F. Bowyer ◽  
Monzy Thomas ◽  
Tucker A. Patterson ◽  
Nysia I. George ◽  
Jeffrey A. Runnells ◽  
...  

1993 ◽  
Vol 264 (3) ◽  
pp. R513-R523 ◽  
Author(s):  
J. Brown ◽  
Z. Zuo

Natriuretic peptide receptors in rat brain were mapped by in vitro autoradiography using 125I-labeled [Tyr0]CNP-(1-22) to bind atrial natriuretic peptide receptor (ANPR)-B and ANPR-C receptors selectively, and 125I-labeled alpha-ANP to select ANPR-A and ANPR-C receptors. Des-[Gln18,Ser19,Gly20,Leu21,Gly22]ANP-(4- 23)-amide (C-ANP) was used for its selectivity for ANPR-C over ANPR-A. Specific binding of 125I-[Tyr0]CNP-(1-22) with a dissociation constant (Kd) approximately 1 nM occurred in olfactory bulb, cerebral cortex, lateral septal nucleus, choroid plexus, and arachnoid mater. This binding was abolished by C-type natriuretic peptide [CNP-(1-22)], alpha-ANP and C-ANP, and conformed to ANPR-C. 125I-alpha-ANP bound to all structures that bound 125I-[Tyr0]CNP-(1-22). This binding was also inhibited by both CNP-(1-22) and C-ANP, confirming the presence of ANPR-C-like binding sites. However, ANPR-C-like binding sites were heterogenous because only some had high affinities for 125I-[Tyr0]CNP-(1-22) and CNP-(1-22). 125I-alpha-ANP also bound sites without affinities for C-ANP or CNP-(1-22). These sites were consistent with ANPR-A. They occurred mainly on the olfactory bulb, the choroid plexus, and the subfornical organ. Guanosine 3',5'-cyclic monophosphate production was strongly stimulated by alpha-ANP but not by CNP-(1-22) in olfactory bulb. Neither ligand stimulated it in cortical tissue. Thus the natriuretic peptide binding sites of rat brain conformed to ANPR-A and to heterogenous ANPR-C-like sites. No ANPR-B were detected.


Sign in / Sign up

Export Citation Format

Share Document