scholarly journals The role of myoendothelial cell contact in non-nitric oxide-, non-prostanoid-mediated endothelium-dependent relaxation of porcine coronary artery

1994 ◽  
Vol 113 (4) ◽  
pp. 1289-1294 ◽  
Author(s):  
Elisabeth Kühberger ◽  
Klaus Groschner ◽  
Walter R. Kukovetz ◽  
Friedrich Brunner
Keyword(s):  
Nitric Oxide ◽  
Coronary Artery ◽  
Cell Contact ◽  
Porcine Coronary Artery ◽  
Endothelium Dependent Relaxation

Two mechanisms mediate relaxation by bradykinin of pig coronary artery: NO-dependent and -independent responses

1991 ◽  
Vol 261 (3) ◽  
pp. H830-H835 ◽  
Author(s):  
C. L. Cowan ◽  
R. A. Cohen
Keyword(s):  
Nitric Oxide ◽  
Methylene Blue ◽  
Coronary Artery ◽  
Thromboxane A2 ◽  
Porcine Coronary Artery ◽  
Cyclic Monophosphate ◽  
Pig Coronary Artery ◽  
Endothelium Dependent Relaxation

The role of nitric oxide and guanosine 3',5'-cyclic monophosphate (cGMP) accumulation in the endothelium-dependent relaxation of the porcine coronary artery to bradykinin was investigated by comparing relaxation and cGMP accumulation in the presence or absence of NG-monomethyl-L-arginine (L-NMMA) and methylene blue. Rings were treated with indomethacin to eliminate the effects of prostaglandins. Relaxation to bradykinin of rings contracted with the thromboxane A2 mimetic U-46619 was not affected by L-NMMA and was only minimally inhibited by methylene blue. Rings contracted with elevated potassium (25 mM) also relaxed completely to bradykinin. However, L-NMMA or methylene blue effectively inhibited relaxation to bradykinin in rings contracted with potassium. cGMP accumulation was stimulated by bradykinin and inhibited by L-NMMA or methylene blue in rings contracted with either U-46619 or potassium. These results suggest that in the absence of nitric oxide-induced cGMP accumulation, a nonprostanoid mechanism exists that is capable of completely relaxing U-46619-contracted coronary artery. This mechanism is either inhibited in or unable to relax potassium-contracted rings. These results also demonstrate that nitric oxide mediates the bradykinin-induced cGMP accumulation that is largely responsible for the relaxation during contraction with potassium.

scholarly journals Cilnidipine, a slow-acting Ca2+ channel blocker, induces relaxation in porcine coronary artery: role of endothelial nitric oxide and [Ca2+ ]i

2006 ◽  
Vol 147 (1) ◽  
pp. 55-63 ◽  
Author(s):  
Hok Sum Leung ◽  
Xiaoqiang Yao ◽  
Fung Ping Leung ◽  
Wing Hung Ko ◽  
Zhen-Yu Chen ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Coronary Artery ◽  
Channel Blocker ◽  
Porcine Coronary Artery ◽  
Endothelial Nitric Oxide

Effects of L-arginine analogues on vasomotion of isolated porcine coronary arteries

1995 ◽  
Vol 268 (5) ◽  
pp. H1966-H1972 ◽  
Author(s):  
R. Nakaike ◽  
H. Shimokawa ◽  
H. Yasutake ◽  
H. Sumimoto ◽  
A. Ito ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Coronary Artery ◽  
Coronary Arteries ◽  
Isometric Tension ◽  
Vascular Responses ◽  
Porcine Coronary Artery ◽  
Coronary Vasomotion ◽  
Vasoconstrictor Effect ◽  
Dose Dependent

L-Arginine analogues have been widely used to examine the role of endothelium-derived nitric oxide (NO) in vascular responses; however, the effects of the agents on coronary vasomotion are not fully understood. In this study, we examined the effects of the analogues on vasomotion of isolated porcine coronary arteries. Strips of the porcine coronary artery were suspended for isometric tension recording in Krebs-Henseleit solution. L-Arginine analogues, N omega-nitro-L-arginine methyl ester (L-NAME, 10(-9)-10(-3) M), NG-monomethyl-L-arginine (L-NMMA, 10(-9)-10(-3) M), and NG-nitro-L-arginine (L-NNA, 10(-9)-10(-3) M), caused dose-dependent contractions, which were greater in strips with than in those without endothelium. Those endothelium-dependent contractions were almost abolished by indomethacin (10(-5) M) and FeCl2 (10(-3) M). The latter reduces prostaglandin H2 to 12-heptadecatrienoic acid, which has no vasoconstrictor effect. These results indicate that the L-arginine analogues cause endothelium-dependent contractions that are mediated by prostaglandin endoperoxides and suggest that they have properties other than simple inhibition of NO synthesis in porcine coronary arteries.

Effects of Thiopental on Endothelium-Dependent Relaxation in Porcine Coronary Arteries

2009 ◽  
Vol 37 (4) ◽  
pp. 1011-1017 ◽  
Author(s):  
O Dagtekin ◽  
HJ Gerbershagen ◽  
E Özgür ◽  
J Gaertner ◽  
JH Fischer
Keyword(s):  
Nitric Oxide ◽  
Coronary Artery ◽  
Coronary Arteries ◽  
Relaxation Response ◽  
Nitric Oxide Production ◽  
Porcine Coronary Artery ◽  
Relevant Concentration ◽  
Oxide Production ◽  
Prostaglandin F ◽  
Endothelium Dependent Relaxation

This study investigated the effects of thiopental on endothelium-dependent relaxation (EDR), and especially the effects on nitric oxide-and prostacyclin-independent EDR. Fresh porcine coronary artery rings (4 mm long), were consecutively tested with and without 20 μg/ml thiopental in Krebs–Henseleit solution. Indomethacin (10 μmol/1) was used in all experiments to eliminate prostacyclin effects. Prostaglandin F2α (10 μmol/l) was used to induce contractions and bradykinin (10−10−10−5 M) was used to induce EDR. Experiments were also carried out using 300 μmol/1 N-nitro-l-arginine to block nitric oxide production and to assess the influence of thiopental on nitric oxide-and prostacyclin-independent EDR. Thiopental induced statistically significant increases in EDR at concentrations of 10−6−10−5 M bradykinin. Following nitric oxide production block, thiopental significantly reduced the relaxation response at concentrations of 10−8−10−5 M bradykinin. At a clinically relevant concentration of 20 μg/ml thiopental, a significant increase in EDR and a significant reduction in nitric oxide-and prostacyclin-independent relaxation was observed in porcine epicardial coronary arteries.

scholarly journals Urocortin-induced endothelium-dependent relaxation of rat coronary artery: role of nitric oxide and K+ channels

2002 ◽  
Vol 135 (6) ◽  
pp. 1467-1476 ◽  
Author(s):  
Yu Huang ◽  
Franky Leung Chan ◽  
Chi-Wai Lau ◽  
Suk-Ying Tsang ◽  
Guo-Wei He ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Coronary Artery ◽  
K Channels ◽  
Endothelium Dependent Relaxation

scholarly journals Role of sulfhydryl-dependent dimerization of soluble guanylyl cyclase in relaxation of porcine coronary artery to nitric oxide

2011 ◽  
Vol 90 (3) ◽  
pp. 565-572 ◽  
Author(s):  
X. Zheng ◽  
L. Ying ◽  
J. Liu ◽  
D. Dou ◽  
Q. He ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Coronary Artery ◽  
Guanylyl Cyclase ◽  
Soluble Guanylyl Cyclase ◽  
Porcine Coronary Artery
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