Selection of Lactobacillus fermentum strains able to durably colonize the digestive tract of mice harboring a complex human flora

Probiotics are friendly bacteria benefit the health which occur naturally in the digestive tract of human beings. Prebiotics, act as ‘food’ for these beneficial probiotic bacteria, promoting their activity. Lactobacillus and Bifidobacterium are widely used probiotics which generally regarded as safe. They are adhesive to mucosal and epithelial surfaces and enhance immunity by preventing pathogen adhesion in the digestive system. Although infections with probiotics are very rare, few research studies have been reported in children and also in elderly suffering from diseases due to supplementation of few types of species which may be due to immunodeficiency and immunosuppression. The familiarity of probiotics is still uncertain in many and they doubt whether to consume or not. This review provides the information based on research studies and gives clarity on the intake of probiotics. However, caution need to be exercised in the selection of genus and strains of particular probiotics genera.

Download Full-text

Selective decontamination of the digestive tract (SDD), ICU-acquired pneumonia and selection of antibiotic-resistant bacteria

Journal of Hospital Infection ◽

10.1016/0195-6701(90)90132-8 ◽

1990 ◽

Vol 15 (2) ◽

pp. 195-197 ◽

Cited By ~ 2

Author(s):

S.R. Alcock ◽

D.S. Cole

Keyword(s):

Digestive Tract ◽

Selective Decontamination ◽

Resistant Bacteria ◽

Antibiotic Resistant Bacteria ◽

Antibiotic Resistant ◽

Selection Of

Download Full-text

In Vitro Antagonistic Effect of Gut Bacteriota Isolated from Indigenous Honey Bees and Essential Oils against Paenibacillus Larvae

International Journal of Molecular Sciences ◽

10.3390/ijms21186736 ◽

2020 ◽

Vol 21 (18) ◽

pp. 6736 ◽

Cited By ~ 1

Author(s):

Miroslava Kačániová ◽

Margarita Terentjeva ◽

Jana Žiarovská ◽

Przemysław Łukasz Kowalczewski

Keyword(s):

Antimicrobial Activity ◽

Essential Oils ◽

Digestive Tract ◽

Antagonistic Effect ◽

Paenibacillus Larvae ◽

Thymus Vulgaris ◽

Plant Essential Oils ◽

Microbial Groups ◽

Selection Of

The aim of study was to isolate and identify the gut bacteria of Apis mellifera and to evaluate antagonistic effect of the bacteriota against Paenibacillus larvae, which causes American foulbrood (AFB) in honeybees. The dilution plating method was used for the quantification of selected microbial groups from digestive tract of bees, with an emphasis on the bacteriota of the bees’ intestines. Bacteria were identified using mass spectrometry (MALDI-TOF-MS Biotyper). Overall, five classes, 27 genera and 66 species of bacteria were identified. Genera Lactobacillus (10 species) and Bacillus (8 species) were the most abundant. Gram-negative bacteria were represented with 16 genera, whereas Gram-positive with 10 genera. Delftia acidovorans and Escherichia coli were the most abundant in the digestive tract of honey bee. Resistance to a selection of antimicrobials was assessed for the bacterial isolates from bee gut and confirmed against all antimicrobials included in the study, with the exception of cefepime. Lactobacillus spp., especially L. kunkeei, L. crispatus and L. acidophilus. showed the strongest antimicrobial activity against P. larvae, the causal pathogen of AFB. Antimicrobial activity of essential oils against isolated bacteria and two isolates of P. larvae were assessed. Application of a broad selection of plant essential oils indicated that Thymus vulgaris had the highest antimicrobial activity against P. larvae.

Download Full-text

Study on digestive tract contents of fish : Preliminary step for identification of indigenous species in mosquito larval control

Journal of Applied and Natural Science ◽

10.31018/jans.v1i1.26 ◽

2009 ◽

Vol 1 (1) ◽

pp. 18-23

Author(s):

N. Pemola Devi ◽

R. K. Jauhari

Keyword(s):

Digestive Tract ◽

Natural Condition ◽

Food Preference ◽

Indigenous Species ◽

Natural Habitats ◽

Preliminary Step ◽

Larval Control ◽

Larvivorous Fish ◽

Selection Of

The present study is based on screening of food preference by the indigenous fish under its natural condition. The gastrointestinal contents as an indicative of effective bioregulatory activity envisage the selection of a larvivorous fish. As many as 32 species of fishes were collected from their natural habitats at Imphal and Bishenpur districts in Manipur State between August 2007 to February 2008. Faecal drops of active fishes revealed remains of larvae / pupae of mosquitoes. However, the food types in 26 species of fish comprised larvae / pupae of mosquito, algae, weeds, tadepole fishes, crustaceans, insects, gastropods, worms and detritus / debris. On the basis of food preference Aplocheilus panchax, Polyacanthus fasciatus and Puntius manipurensis have been considered as most potent larvivorous fish of the study area.

Download Full-text

Competitive Selection of Lactic Acid Bacteria That Persist in the Human Oral Cavity

Applied and Environmental Microbiology ◽

10.1128/aem.06043-11 ◽

2011 ◽

Vol 77 (23) ◽

pp. 8445-8450 ◽

Cited By ~ 8

Author(s):

Johannes Snel ◽

Maria L. Marco ◽

Fedde Kingma ◽

Wouter M. Noordman ◽

Jan Rademaker ◽

...

Keyword(s):

Lactic Acid ◽

Lactic Acid Bacteria ◽

Oral Cavity ◽

Lactobacillus Fermentum ◽

Lactobacillus Salivarius ◽

Content Type ◽

Competitive Selection ◽

Human Oral Cavity ◽

Selection Of

ABSTRACTLactic acid bacteria (LAB) might offer opportunities as oral probiotics provided candidate strains persist in the mouth. After intake of a mixture of 69 LAB, strains ofLactobacillus fermentumandLactobacillus salivariuswere especially recovered. Coaggregation with other microbes is likely not a prerequisite for persistence sinceL. salivariusstrongly coaggregated with typical oral cavity isolates, whereasL. fermentumfailed to display this phenotype.

Download Full-text

Epizootic situation and peculiarity the course nematodes of the digestive canal of sheep of the in the conditions of economies Kyev region

Naukovij vìsnik veterinarnoï medicini ◽

10.33245/2310-4902-2019-149-1-75-84 ◽

2019 ◽

pp. 75-84

Author(s):

V. MELNYCHUK ◽

A. Antipov

Keyword(s):

Species Composition ◽

Digestive Tract ◽

Economic Losses ◽

Parasitic Diseases ◽

Prevention Measures ◽

Treatment And Prevention ◽

Invasive Diseases ◽

Effective Cost ◽

Successful Control ◽

Selection Of

Nematodoses of the sheep’s digestive tract is distributed both in Ukraine and abroad. Pathogens of these diseases in the form of monoinvasions, as well as associative with other invasive diseases, cause sheep farms considerable economic losses. Systematic monitoring studies that are carried out to establish a helminthofauna specific to a particular region are a key to successful control of parasitic diseases. Information on the species composition of pathogens causing invasive diseases in animals in the territory of a certain region, allows us to conduct scientifically based selection of drugs and to conduct effective, cost-based treatment and prevention measures. Therefore, the purpose of our research was to establish the distribution of nematodoses of the digestive canal of sheep and their peculiarities in the conditions of sheep farms in the territory of the Kyiv region (Central region of Ukraine). The research was conducted in conditions of sheep farms in the Kyiv region (Baryshivka, Bila Tserkva, Zghurivka, Pereiaslav-Khmelnytskyi, Skvyra, Tarashcha, Fastiv and Yahotyn districts). Helminthoscopy of fecal samples was performed using the McMaster’s method. As a result of scatoscopy diagnostics of sheep, a considerable distribution of nematodoses of the digestive canal was established on the territory of the Kyiv region (the severity of the invasion was 48.79 %). According to the morphological features of eggs isolated from faeces of sick animals, parasitization of nematodes from three sequences was established: Strongylata (38.98 %), Trichocephalata (33.36 %), and Rhabditata (27.66 %). It was registered that the sheep farming of the Bila Tserkva, Yahotyn and Zghurivka districts (EI – 61.49, 59.73 and 54.17 % respectively) were the most unfavorable for nematodoses of the digestive tract. The disease was registered in the form of both mono- and mixinvasions. The associative course of diseases (75.03 % of the total number of diseased animals) was dominant with two-, three-, four-, and five-component associations of sheep parasite pathogens (47.63, 35.47, 11.50 and 5.40 % in accordance). It has been established that nematodoses of the digestive canal of sheep (strongylates of the digestive canal, strongyloides and trichiuruses) are in the composition of mixinvasions, the sympathizers of which are: eimeries, moniezies, and melofagoses in various combinations. Key words: nematodoses, strongylatoses of the digestive canal, trichiuruses, strongyloides, scatoscopy research.

Download Full-text

Pro- and synbiotics in the treatment of intestinal diseases: what effects can we expect?

Consilium Medicum ◽

10.26442/20751753.2020.12.200309 ◽

2020 ◽

Vol 22 (12) ◽

pp. 37-43

Author(s):

Olga V. Gaus ◽

◽

Maria A. Livzan ◽

Keyword(s):

Clinical Practice ◽

Digestive Tract ◽

Diagnostic Methods ◽

Intestinal Diseases ◽

Bowel Diseases ◽

Inflammatory Bowel ◽

The Relationship ◽

Selection Of ◽

Real Clinical Practice

The relationship between intestinal microbiota and human health is widely recognized. The advent of molecular and genetic diagnostic methods has made it po-ssible to make truly revolutionary discoveries in the field of understanding the role of the digestive tract microbiome. Many studies have proved the association of the composition of microbiota with the development of a number of diseases of the digestive tract, such as inflammatory bowel diseases, functional gastroin-testinal disorders and some types of cancer. The food and pharmaceutical industry has presented a huge selection of food products, dietary supplements and medicines containing prebiotic and probiotic components. The use of agents that affect microbiota in the treatment of intestinal diseases in real clinical practice should be carried out strictly in accordance with the indications, in recommended doses.

Download Full-text

Selection of nerolidol among a series of terpenic and phenolic compounds for its potent activity against Lactobacillus fermentum ATCC 9338

Process Biochemistry ◽

10.1016/j.procbio.2019.02.015 ◽

2019 ◽

Vol 80 ◽

pp. 146-156 ◽

Cited By ~ 6

Author(s):

Elissa Ephrem ◽

Amal Najjar ◽

Catherine Charcosset ◽

Hélène Greige-Gerges

Keyword(s):

Phenolic Compounds ◽

Lactobacillus Fermentum ◽

Selection Of

Download Full-text

Lactobacillus casei Is Able To Survive and Initiate Protein Synthesis during Its Transit in the Digestive Tract of Human Flora-Associated Mice

Applied and Environmental Microbiology ◽

10.1128/aem.68.7.3570-3574.2002 ◽

2002 ◽

Vol 68 (7) ◽

pp. 3570-3574 ◽

Cited By ~ 65

Author(s):

R. Oozeer ◽

N. Goupil-Feuillerat ◽

C. A. Alpert ◽

M. van de Guchte ◽

J. Anba ◽

...

Keyword(s):

Protein Synthesis ◽

Human Health ◽

Digestive Tract ◽

Lactobacillus Casei ◽

Light Emission ◽

Luciferase Activity ◽

Fermented Milk ◽

Luciferase Expression ◽

Erythromycin Resistance ◽

Human Flora

ABSTRACT Live Lactobacillus casei is present in fermented dairy products and has beneficial properties for human health. In the human digestive tract, the resident flora generally prevents the establishment of ingested lactic acid bacteria, the presence of which is therefore transient. The aim of this work was to determine if L. casei DN-114 001 survives during transit and how this bacterium behaves in the digestive environment. We used the human flora-associated (HFA) mouse model. L. casei DN-114 001 was genetically modified by the introduction of erm and lux genes, encoding erythromycin resistance and luciferase, respectively. For this modified strain (DN-240 041), light emission related to luciferase expression could easily be detected in the contents of the digestive tract. When inoculated into the digestive tract of HFA mice, L. casei (DN-240 041) survives but is eliminated with the same kinetics as an inert transit marker, indicating that it does not establish itself. In pure culture of L. casei, luciferase activities were high in the exponential and early stationary growth phases but decreased to become undetectable 1 day after inoculation. Viability was only slightly reduced even after more than 5 days. After transit in HFA mice, luciferase activity was detected even when 5-day-old L. casei cultures were given to the mice. In culture, the luciferase activity could be restored after 0.5 to 7 h of incubation in fresh medium or milk containing glucose, unless protein synthesis was inhibited by the addition of chloramphenicol or rifampin. These results suggest that in HFA mice L. casei DN-240 041, and thus probably L. casei DN-114 001, is able to initiate new protein synthesis during its transit with the diet. The beneficial properties of L. casei-fermented milk for human health might be related to this protein synthesis in the digestive tract.

Download Full-text