Genetic Characterization of Enterococcus Faecalis N1-33 Bacteriocin and Obtained of the Mutant Strain that Produce Many Bacteriocin by Novobiocin Agent Effect, its Merit as A Food Preservative in Steamed Rice Model

2015 ◽  
Vol 36 (3) ◽  
pp. 348-359 ◽  
Author(s):  
Tomomi Hata ◽  
Toyoki Sato ◽  
Yasujiro Morimitsu
Keyword(s):  
Enterococcus Faecalis ◽  
Mutant Strain ◽  
Genetic Characterization ◽  
Food Preservative

Characterization of a Bacteriocin Produced by Enterococcus faecalis N1-33 and Its Application as a Food Preservative

2009 ◽  
Vol 72 (3) ◽  
pp. 524-530 ◽  
Author(s):  
TOMOMI HATA ◽  
MELAKU ALEMU ◽  
MIHO KOBAYASHI ◽  
CHISE SUZUKI ◽  
SUNEE NITISINPRASERT ◽  
...  
Keyword(s):  
Enterococcus Faecalis ◽  
Marked Reduction ◽  
Pathogenic Bacteria ◽  
Culture Supernatant ◽  
Amino Acid Residues ◽  
Bacteriocin Activity ◽  
Food Preservative ◽  
Fermented Bamboo Shoot ◽  
Potential Use

A bacteriocin-producing strain, N1-33, isolated from fermented bamboo shoot was identified as Enterococcus faecalis. The pH-adjusted culture supernatant of this strain consisted of several peptides with bacteriocin activity, and the supernatant inhibited the growth of pathogenic bacteria such as Listeria monocytogenes. The major peptide with bacteriocin activity was purified, and the first 39 amino acid residues of the bacteriocin were found to be identical to enterocin MR10A produced by E. faecalis MRR10-3. Addition of the pH-adjusted and concentrated culture supernatant of strain N1-33 caused a marked reduction in the growth of Bacillus cereus in custard cream and L. monocytogenes in pickled cucumber. These results suggest the potential use of the bacteriocin produced by strain N1-33 in food biopreservation.

scholarly journals Biochemical and Genetic Characterization of the Two-Peptide Bacteriocin Enterocin 1071 Produced by Enterococcus faecalis FAIR-E 309

2002 ◽  
Vol 68 (5) ◽  
pp. 2550-2554 ◽  
Author(s):  
Charles M. A. P. Franz ◽  
Alexandra Grube ◽  
Anette Herrmann ◽  
Hikmate Abriouel ◽  
Joachim Stärke ◽  
...  
Keyword(s):  
Amino Acids ◽  
Sequence Analysis ◽  
Enterococcus Faecalis ◽  
Dna Sequence ◽  
Genetic Characterization ◽  
Peptide Sequence ◽  
Transporter Gene ◽  
Structural Genes ◽  
Heterologous Host

ABSTRACT The structural genes for the two-peptide bacteriocin enterocin 1071 (Ent1071) in Enterococcus faecalis FAIR-E 309 were cloned. DNA sequence analysis showed that the enterocin 1071A (Ent1071A) peptide of strain FAIR-E 309 differed by two amino acids from the Ent1071A reported for E. faecalis BFE 1071 (E. Balla, L. M. T. Dicks, M. Du Toit, M. J. van der Merwe, and W. H. Holzapfel, Appl. Environ. Microbiol. 66:1298-1304, 2000), while the Ent1071B gene encoded identical peptides in these strains. However, resequencing of ent1071A from E. faecalis BFE 1071 showed that the Ent1071A peptide sequence reported previously was incorrect in two amino acids. Also, ent1071B in E. faecalis FAIR-E 309 encoded a prepeptide that was three amino acids shorter than that previously reported for E. faecalis BFE 1071 Ent1071B. A presumptive immunity gene (eni1071) was located downstream of the bacteriocin structural genes. This gene was cloned into the heterologous host E. faecalis ATCC 19433 and was shown to confer immunity. A truncated ABC transporter gene was located upstream of the Ent1071 structural genes.

Genetic characterization of high-level gentamicin-resistant strains of Enterococcus faecalis in Iran

2004 ◽  
Vol 50 (10) ◽  
pp. 869-872 ◽  
Author(s):  
Mohammad Mehdi Feizabadi ◽  
Sorour Asadi ◽  
Maryam Zohari ◽  
Sara Gharavi ◽  
Gelavizh Etemadi
Keyword(s):  
Genetic Variation ◽  
Enterococcus Faecalis ◽  
Genetic Characterization ◽  
Enzyme Electrophoresis ◽  
Multilocus Enzyme Electrophoresis ◽  
Gentamicin Resistance ◽  
Resistant Strains ◽  
Plasmid Profiling ◽  
High Level

The prevalence of resistance to high levels of gentamicin among 182 isolates of Enterococcus faecalis from 2 Iranian hospitals was 42%. Gentamicin resistance was associated with conjugative plasmids (>70 kb) in most strains. Fingerprinting using EcoRI and HindIII showed genetic variation among these plasmids and gave evidence of nosocomial outbreaks and persistence of infection in different wards of the study hospitals, as well as transfer of plasmids between genetically diverse isolates. Using EcoRI, hospital-based specific plasmid fingerprints were detected for the isolates that had previously proved to be unrelated by multilocus enzyme electrophoresis, suggesting the persistence of related plasmids at each hospital, though minor changes in these related plasmids could be detected with HindIII.Key words: Enterococcus faecalis, HLGR, plasmid profiling, plasmid fingerprinting.

Histopathological and genetic characterization of colorectal mucinous carcinoma

2001 ◽  
Vol 120 (5) ◽  
pp. A166-A166
Author(s):  
S FUJII ◽  
T KUSAKA ◽  
T KAIHARA ◽  
Y UEDA ◽  
T CHIBA ◽  
...  
Keyword(s):  
Genetic Characterization ◽  
Mucinous Carcinoma

Genetic characterization of high hyperdiploidy in childhood acute lymphoblastic leukemia

2009 ◽  
Vol 221 (03) ◽  
Author(s):  
R Vagkopoulou ◽  
C Eckert ◽  
U Ungethüm ◽  
G Körner ◽  
M Stanulla ◽  
...  
Keyword(s):  
Acute Lymphoblastic Leukemia ◽  
Genetic Characterization ◽  
Lymphoblastic Leukemia ◽  
Childhood Acute Lymphoblastic Leukemia

TBE in Sweden

2020 ◽  
Keyword(s):  
Genetic Characterization ◽  
Immunoglobulin M ◽  
Enzyme Linked Immunosorbent Assay ◽  
Second Phase ◽  
Serum Samples ◽  
Tick Borne Encephalitis ◽  
Specific Immunoglobulin ◽  
Tick Borne Encephalitis Virus ◽  
First Time

Tick-borne encephalitis virus (TBEV) was isolated for the first time in Sweden in 1958 (from ticks and from 1 tick-borne encephalitis [TBE] patient).1 In 2003, Haglund and colleagues reported the isolation and antigenic and genetic characterization of 14 TBEV strains from Swedish patients (samples collected 1991–1994).2 The first serum sample, from which TBEV was isolated, was obtained 2–10 days after onset of disease and found to be negative for anti-TBEV immunoglobulin M (IgM) by enzyme-linked immunosorbent assay (ELISA), whereas TBEV-specific IgM (and TBEV-specific immunoglobulin G/cerebrospinal fluid [IgG/CSF] activity) was demonstrated in later serum samples taken during the second phase of the disease.

Clinical and genetic characterization of two cases of central hypogonadism in Klinefelter syndrome

2020 ◽  
Author(s):  
Rita Indirli ◽  
Biagio Cangiano ◽  
Eriselda Profka ◽  
Elena Castellano ◽  
Giovanni Goggi ◽  
...  
Keyword(s):  
Genetic Characterization ◽  
Klinefelter Syndrome
Sign in / Sign up

Export Citation Format

Share Document