Isolation and characterization of exosomes from adipose tissue‐derived mesenchymal stem cells

2020 ◽  
Author(s):  
Elsa González‐Cubero ◽  
María Luisa González‐Fernández ◽  
Laura Gutiérrez‐Velasco ◽  
Eliezer Navarro‐Ramírez ◽  
Vega Villar‐Suárez
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Isolation And Characterization

scholarly journals Comparison of biological features of wild European rabbit mesenchymal stem cells derived from different tissues.

2021 ◽  
Author(s):  
Marta Díaz-de Frutos ◽  
Alexandra Calle ◽  
María Zamora-Ceballos ◽  
Juan Bárcena ◽  
Esther Blanco ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
European Rabbit ◽  
Isolation And Characterization ◽  
Expression Characteristic ◽  
Subcutaneous Adipose ◽  
Different Tissues

Although the European rabbit is an "endangered" species and a notorious biological model, the analysis and comparative characterization of new tissue sources of rabbit mesenchymal stem cells (rMSCs) has not been well studied. Here we report for the first time the isolation and characterization of rMSCs derived from an animal belonging to a natural rabbit population within the species native region. New rMSC lines were isolated from different tissues: oral mucosa (rOM-MSC), dermal skin (rDS-MSC), subcutaneous adipose tissue (rSCA-MSC), ovarian adipose tissue (rOA-MSC), oviduct (rO-MSC), and mammary gland (rMG­MSC). The six rMSC lines showed plastic adhesion with fibroblast-like morphology and were all shown to be positive for CD44 and CD29 expression (characteristic markers of MSCs), and negative for CD34 or CD45 expression. In terms of pluripotency features, all rMSC lines expressed NANOG, OCT4, and SOX2. Furthermore, all rMSC lines cultured under osteogenic, chondrogenic, and adipogenic conditions showed differentiation capacity. In conclusion, this study describes the isolation and characterization of new rabbit cell lines from different tissue origins, with a clear mesenchymal pattern. We show that rMSC do not exhibit differences in terms of morphological features, expression of the cell surface, and intracellular markers of pluripotency and in vitro differentiation capacities, attributable to their tissue of origin.

scholarly journals Isolation and characterization of bone marrow-derived mesenchymal stem cells in Xenopus laevis

2021 ◽  
pp. 102341
Author(s):  
Rina Otsuka-Yamaguchi ◽  
Masaaki Kitada ◽  
Yasumasa Kuroda ◽  
Yoshihiro Kushida ◽  
Shohei Wakao ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Xenopus Laevis ◽  
Isolation And Characterization

scholarly journals Isolation and characterization of turkey bone marrow‐derived mesenchymal stem cells

2019 ◽  
Vol 37 (6) ◽  
pp. 1419-1428
Author(s):  
Qian Liu ◽  
Yaxi Zhu ◽  
Jun Qi ◽  
Peter C. Amadio ◽  
Steven L. Moran ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Isolation And Characterization

scholarly journals Isolation and Characterization of Mesenchymal Stem Cells from Human Liposuction Aspirate

2019 ◽  
Vol 22 (2) ◽  
pp. 136-143
Author(s):  
Alaa Eldin Abdelhamid ◽  
Mohamed El Shahedy ◽  
Mona Mohammed ◽  
Ghada Aly ◽  
Bassma Dessouki
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Isolation And Characterization

scholarly journals Establishing a technique for isolation and characterization of human periodontal ligament derived mesenchymal stem cells

2020 ◽  
Author(s):  
Spoorthi Ravi Banavar ◽  
Swati Yeshwant Rawal ◽  
Ian Charles Paterson ◽  
Gurbind Singh ◽  
Fabian Davamani ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Periodontal Ligament ◽  
Isolation And Characterization

scholarly journals Isolation and characterization of multipotent mesenchymal stem cells in nasal polyps

2014 ◽  
Vol 240 (2) ◽  
pp. 185-193 ◽  
Author(s):  
Jung-Sun Cho ◽  
Joo-Hoo Park ◽  
Ju-Hyung Kang ◽  
Sung Eun Kim ◽  
Il-Ho Park ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Nasal Polyps ◽  
Isolation And Characterization ◽  
Multipotent Mesenchymal Stem Cells
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