Effectiveness of antimicrobial photodynamic therapy as an adjunct to mechanical instrumentation in reducing counts of Enterococcus faecalis and Candida albicans from C‐shaped root canals

2021 ◽  
Author(s):  
Mohammed Mustafa ◽  
Hadi Mohammed Alamri ◽  
Ahmed A. Almokhatieb ◽  
Ali Robaian Alqahtani ◽  
Abdullah S. Alayad ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Photodynamic Therapy ◽  
Enterococcus Faecalis ◽  
Antimicrobial Photodynamic Therapy ◽  
Root Canals

Efficacy of antimicrobial photodynamic therapy for root canals infected with Enterococcus faecalis

2014 ◽  
Vol 56 (4) ◽  
pp. 277-285 ◽  
Author(s):  
Ricardo A. S. Arneiro ◽  
Ryan D. Nakano ◽  
Lívia A. A. Antunes ◽  
Gustavo B. Ferreira ◽  
Karla B. F. C. Fontes ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Enterococcus Faecalis ◽  
Antimicrobial Photodynamic Therapy ◽  
Root Canals

scholarly journals Effect of photodynamic therapy using diode laser and a novel plant extract irrigant against Enterococcus faecalis and Candida albicans

2021 ◽  
Vol 45 (1) ◽  
Author(s):  
Doaa M. Sadony ◽  
Maram E. Khallaf ◽  
Jackline Samir Kamal
Keyword(s):  
Methylene Blue ◽  
Candida Albicans ◽  
Photodynamic Therapy ◽  
Enterococcus Faecalis ◽  
Moringa Oleifera ◽  
Bacterial Count ◽  
Control Group ◽  
Root Canals ◽  
Laser Group ◽  
Group 10

Abstract Background To evaluate the antibacterial effect of diode laser and photodynamic therapy using methylene blue dye when used alone or in combination with Moringa oleifera extract on root canal microbes. A total of 50 single root canals with single straight canals were prepared with ProTaper files, sterilized in an autoclave device, contaminated with root canal microbes (Enterococcus faecalis and Candidia albicans) and placed in incubator for 21 days. They were then randomly divided into 6 groups. Laser group: (10 root canals) were subjected to laser only. Moringa group: (10 root canals) were irrigated using plant extract only (Moringa). PDT group: (10 root canals) were irrigated using Moringa and Methylene blue then subjected to laser. Methylene blue and laser group: (10 root canals) were irrigated using methylene blue then subjected to laser. Negative control group: (10 root canals) weren't irrigated. After treatment microbial assessment (colony count) was done. Data was analyzed using ANOVAs test. Results Moringa/laser group and PDT group showed statistically least bacterial count for both E. faecalis and Candida albicans. Moringa only group showed statistically less bacterial count than control group. Conclusion The combined use of PDT together with Moringa oleifera has the best antimicrobial effect.

scholarly journals The Effect of Antimicrobial Photodynamic Therapy Using Chlorophyllin–Phycocyanin Mixture on Enterococcus faecalis: The Influence of Different Light Sources

2020 ◽  
Vol 10 (12) ◽  
pp. 4290 ◽  
Author(s):  
Nasim Chiniforush ◽  
Maryam Pourhajibagher ◽  
Steven Parker ◽  
Stefano Benedicenti ◽  
Abbas Bahador ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Diode Laser ◽  
Enterococcus Faecalis ◽  
Control Group ◽  
Human Gingival Fibroblast ◽  
Light Sources ◽  
Gingival Fibroblast ◽  
Antimicrobial Photodynamic Therapy ◽  
Cell Viability Assay ◽  
Vitro Effect

The purpose of this study was to evaluate the in vitro effect of the chlorophyllin–phycocyanin mixture (Photoactive+) as a photosensitizer (PS) during antimicrobial photodynamic therapy (aPDT) on the count of Enterococcus faecalis (E. faecalis) using different light sources. The antimicrobial effect of aPDT with chlorophyllin–phycocyanin mixture using different light sources including diode laser (λ = 660 nm), diode laser (λ = 635 nm), LED (λ = 450 ± 30 nm) alone or in combination was assessed using microbial cell viability assay against E. faecalis. In addition, the cell cytotoxicity of Photoactive+ was assessed on human gingival fibroblast (HuGu) cells by MTT assay; E. faecalis growth when treated by both red wavelengths (635 nm, 660 nm) and combination of LED (420–480 nm) and red wavelengths (635 nm, 660 nm), significantly reduced compared to the control group (p < 0.05). There was no significant reduction in the number of viable cells exposed to Photoactive+ compared to the control group (p < 0.05). This study shows that the application of chlorophyllin–phycocyanin mixture and irradiation with emission of red light achieved a better result for bacterial count reduction, compared to a control. This component can be applied safely due to very negligible cytotoxicity.

DNase increases the efficacy of antimicrobial photodynamic therapy on Candida albicans biofilms

2019 ◽  
Vol 27 ◽  
pp. 124-131 ◽  
Author(s):  
Beatriz H.D. Panariello ◽  
Marlise I. Klein ◽  
Fernanda Alves ◽  
Ana Cláudia Pavarina
Keyword(s):  
Candida Albicans ◽  
Photodynamic Therapy ◽  
Antimicrobial Photodynamic Therapy ◽  
Candida Albicans Biofilms

scholarly journals Antimicrobial Photodynamic Therapy in Combination with Nystatin in the Treatment of Experimental Oral Candidiasis Induced by Candida albicans Resistant to Fluconazole

2019 ◽  
Vol 12 (3) ◽  
pp. 140 ◽  
Author(s):  
Karem Janeth Rimachi Hidalgo ◽  
Juliana Cabrini Carmello ◽  
Cláudia Carolina Jordão ◽  
Paula Aboud Barbugli ◽  
Carlos Alberto de Sousa Costa ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Photodynamic Therapy ◽  
Combined Therapy ◽  
Oral Candidiasis ◽  
Confocal Scanning Laser Microscopy ◽  
Antimicrobial Photodynamic Therapy ◽  
Promising Alternative ◽  
Confocal Scanning ◽  
Confocal Scanning Laser

Background: It has been demonstrated that azole-resistant strains of Candida albicans have a greater resistance to antimicrobial photodynamic therapy (aPDT) when compared to their more susceptible counterparts. For this reason, the present study evaluated the efficacy of aPDT, together with nystatin (NYS), in the treatment of oral candidiasis in vivo. Methods: Mice were infected with fluconazole-resistant C. albicans (ATCC 96901). To perform the combined therapy, aPDT, mediated by Photodithazine (PDZ) and LED light, was used together with NYS. The efficacy of the treatments was evaluated by microbiological, macroscopic, histopathological and Confocal Scanning Laser Microscopy analyses of the lesions. The expression of p21 and p53, proteins associated with cell death, from the tongues of mice, was also performed. Results: The combined therapy reduced the fungal viability by around 2.6 log10 and decreased the oral lesions and the inflammatory reaction. Additionally, it stimulated the production of p53 and p21. Conclusions: The combined therapy is a promising alternative treatment for oral candidiasis induced by C. albicans resistant to fluconazole.

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