Impact of nucleic acid amplification test on screening of blood donors in Northern Pakistan

Transfusion ◽  
2015 ◽  
Vol 55 (7) ◽  
pp. 1803-1811 ◽  
Author(s):  
Saifullah Khan Niazi ◽  
Farhat Abbas Bhatti ◽  
Nuzhat Salamat ◽  
Eijaz Ghani ◽  
Muhammad Tayyab
2009 ◽  
Vol 59 (2) ◽  
pp. 128-133 ◽  
Author(s):  
Stefano Dettori ◽  
Angela Candido ◽  
Loreta A. Kondili ◽  
Paola Chionne ◽  
Stefania Taffon ◽  
...  

2019 ◽  
Vol 14 (4) ◽  
pp. 387-394
Author(s):  
Aseem Kumar Tiwari ◽  
Ravi C. Dara ◽  
Dinesh Arora ◽  
Geet Aggarwal ◽  
Jyoti Sharma ◽  
...  

Author(s):  
Tade Bagbi ◽  
Ningthoukhongjam Reema ◽  
S. Bhagyabati Devi ◽  
Thangjam Gautam Singh ◽  
Mohammad Jaleel ◽  
...  

Abstract Introduction Tuberculosis (TB) in people living with human immunodeficiency virus (PLHIV) is difficult to diagnose due to fewer organisms in sputum and extrapulmonary samples. Sputum culture takes 4 to 8 weeks for growth of the mycobacteria. Delayed treatment for TB in PLHIV leads to increased mortality. This study evaluated cartridge-based nucleic acid amplification test (CBNAAT) as a diagnostic tool for diagnosis of pulmonary TB (PTB) and extrapulmonary TB (EPTB) in PLHIV in the second most HIV prevalent state in India and for comparing its efficacy between Ziehl–Neelsen (ZN) staining sputum smear–positive and sputum smear–negative TB. Methods This cross-sectional study was conducted in RIMS, Imphal, with 167 PLHIV patients, age 15 years or older, having signs and symptoms of TB. Appropriate samples for sputum microscopy and CBNAAT were sent. Conclusion The overall sensitivity of sputum smear for acid-fast bacillus (AFB) was found to be 30.71% and that of CBNAAT was 38.57%. Sensitivity of CBNAAT for sputum smear–positive and sputum smear–negative TB was 100 and 11.3%, respectively. Sensitivity of ZN smear for AFB of EPTB sample was 48.1% and that of CBNAAT was 59.25%. In both PTB and EPTB, CBNAAT showed an increase in diagnosis of microbiologically confirmed PTB cases by 7.8 and 11.1%, respectively, over and above the cases diagnosed by ZN smear microscopy. Rifampicin resistance was detected in five patients. We conclude that CBNAAT is a rapid test with better sensitivity in diagnosis of PTB and EPTB in PLHIV, compared with ZN smear microscopy. It detects rifampicin resistance for multidrug-resistant TB and helps in early treatment intervention.


Sign in / Sign up

Export Citation Format

Share Document