In vitro chemosensitivity of feline injection site-associated sarcoma cell lines to carboplatin

2017 ◽  
Vol 47 (2) ◽  
pp. 219-226 ◽  
Author(s):  
Elizabeth A. Maxwell ◽  
Heidi Phillips ◽  
David J. Schaeffer ◽  
Timothy M. Fan
Keyword(s):  
Injection Site ◽  
Cell Lines ◽  
Sarcoma Cell ◽  
In Vitro Chemosensitivity

Establishment of two vaccine-associated feline sarcoma cell lines and determination of in vitro chemosensitivity to doxorubicin and mitoxantrone

2001 ◽  
Vol 62 (9) ◽  
pp. 1354-1357 ◽  
Author(s):  
Laurel E. Williams ◽  
Nilanjana Banerji ◽  
Jeffrey S. Klausner ◽  
Vivek Kapur ◽  
Sagarika Kanjilal
Keyword(s):  
Cell Lines ◽  
Sarcoma Cell ◽  
In Vitro Chemosensitivity

Evaluation of in vitro chemosensitivity of vaccine-associated feline sarcoma cell lines to vincristine and paclitaxel

2002 ◽  
Vol 63 (5) ◽  
pp. 728-732 ◽  
Author(s):  
Nilanjana Banerji ◽  
Xia Li ◽  
Jeffrey S. Klausner ◽  
Vivek Kapur ◽  
Sagarika Kanjilal
Keyword(s):  
Cell Lines ◽  
Sarcoma Cell ◽  
In Vitro Chemosensitivity

scholarly journals Sunitinib Exerts In Vitro Immunomodulatory Activity on Sarcomas via Dendritic Cells and Synergizes With PD-1 Blockade

2021 ◽  
Vol 12 ◽  
Author(s):  
Darina Ocadlikova ◽  
Mariangela Lecciso ◽  
Javier Martin Broto ◽  
Katia Scotlandi ◽  
Michele Cavo ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
T Cells ◽  
Dendritic Cells ◽  
T Cell ◽  
Cell Lines ◽  
Effector T Cells ◽  
Sarcoma Cell ◽  
Ifn Γ ◽  
Sarcoma Cells

BackgroundHigh-grade sarcomas are a heterogeneous group of aggressive tumors arising in bone and soft tissues. After relapse, treatment options are limited. The multi-targeted receptor tyrosine kinase inhibitors (TKIs) sunitinib and inhibitor of PD-1 (anti-PD-1) nivolumab have shown antitumor activity in selected subtypes. In this study, we examine the role of TKIs and PD-1 based therapy in in vitro cocultures of sarcoma.MethodsThe human osteosarcoma (SaOS-2) and synovial sarcoma (SYO-1) cell lines were treated with sunitinib. After cell death and proliferation assessment, expression of PD-L1 was analyzed by flow cytometry. Sunitinib-treated sarcoma cells were cocultured with dendritic cells (DCs), and the phenotype of mature DCs was determined by flow cytometry. Mature DCs were cultured with autologous T cells. PD-1 expression on T cells, their proliferation, T regulatory cell (Tregs) induction and IFN-γ production, before and after nivolumab exposure, were analyzed.ResultsAlong with its anti-proliferative and direct pro-apoptotic effect on sarcoma cell lines, sunitinib prompted PD-L1 upregulation on sarcoma cells. Interestingly, sunitinib-treated sarcoma cells drive DCs to full maturation and increase their capacity to induce sarcoma-reactive T cells to produce IFN-γ. Conversely, no effect on T cell proliferation and T cell subpopulation composition was observed. Moreover, both bone and synovial sarcoma cell lines induced Tregs through DCs but sunitinib treatment completely abrogated Treg induction. Finally, sarcoma cell lines induced PD-1 upregulation on both effector T cells and Tregs when loaded into DCs, providing a rationale for using PD-1 blockade. Indeed, PD-1 blockade by nivolumab synergized with sunitinib in inducing IFN-γ-producing effector T cells.ConclusionsTaken together, our in vitro data indicate that the treatment of sarcoma cells with sunitinib can exert significant changes on immune cell subsets toward immune activation, leading to DC-based cross-priming of IFN-γ-producing effector T cells and reduced Treg induction. PD-1 blockade with nivolumab has a synergistic effect with sunitinib, supporting the use of TKI and anti-PD-1 approach in sarcomas, and perhaps in other cancers. DC-targeted drugs, including toll-like receptor 3 inhibitors and CD47 inhibitors, are under development and our preclinical model might help to better design their clinical application.

Masitinib mesylate does not enhance sensitivity to radiation in three feline injection-site sarcoma cell lines under normal growth conditions

2014 ◽  
Vol 96 (2) ◽  
pp. 304-307 ◽  
Author(s):  
M. Turek ◽  
R. Gogal ◽  
C. Saba ◽  
M.L. Vandenplas ◽  
J. Hill ◽  
...  
Keyword(s):  
Injection Site ◽  
Cell Lines ◽  
Normal Growth ◽  
Growth Conditions ◽  
Sarcoma Cell

In vitro radiobiological parameters of human sarcoma cell lines

1988 ◽  
Vol 15 (4) ◽  
pp. 937-942 ◽  
Author(s):  
Ralph R. Weichselbaum ◽  
Michael A. Beckett ◽  
Michael A. Simon ◽  
Carla McCauley ◽  
Daniel Haraf ◽  
...  
Keyword(s):  
Cell Lines ◽  
Sarcoma Cell ◽  
Human Sarcoma
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