An Analysis of the Time-Dependent Changes in Intracellular Calcium Concentration in Endothelial Cells in Culture Induced by Mechanical Stimulation

1993 ◽  
Vol 115 (2) ◽  
pp. 160-168 ◽  
Author(s):  
F. K. Winston ◽  
L. E. Thibault ◽  
E. J. Macarak
Keyword(s):  
Endothelial Cells ◽  
Cell Membrane ◽  
Pulmonary Artery ◽  
Intracellular Calcium ◽  
Mechanical Stimulation ◽  
Calcium Ion ◽  
Time Course ◽  
Mechanical Strain ◽  
Structural Response ◽  
Cells In Culture

When bovine pulmonary artery endothelial cells in culture are subjected to mechanical strain, their physiology is altered. Experimentally, this mechanical strain is generated by increased tension in the substrate to which the cells are attached and results in altered levels of fibronectin. Studies of the structural response of the endothelial cell suggest that this stimulus is transmitted to the cell membrane, organelles, and cytoskeleton by natural cell attachments in a quantifiable and predictable manner. This report examines altered intracellular calcium homeostasis as a possible messenger for the observed strain-induced physiologic response. In particular, using the intracellularly trapped calcium indicator dyes, Quin2 and Fura2, we observed changes in cytosolic free calcium ion concentration in response to biaxial strain of bovine pulmonary artery endothelial cells in culture. The magnitude and time course of this calcium transient resemble that produced by treatment with the calcium ionophore, Ionomycin, indicating that mechanical stimulation may alter cell membrane permeability to calcium. Additional experiments in the presence of EDTA indicated that calcium was also released from intracellular stores in response to strain. In order to explain the stretch-induced calcium transients, a first-order species conservation model is presented that takes into account both the cell’s structural response and the calcium homeostatic mechanisms of the cell. It is hypothesized that the cell’s calcium sequestering and pumping capabilities balanced with its mechanically induced changes in calcium ion permeability will determine the level and time course of calcium accumulation in the cytosol.

Testosterone inhibits bradykinin-induced intracellular calcium kinetics in rat aortic endothelial cells in culture

Steroids ◽  
2002 ◽  
Vol 67 (5) ◽  
pp. 393-397 ◽  
Author(s):  
Ivan Rubio-Gayosso ◽  
Olga Garcia-Ramirez ◽  
Ruth Gutierrez-Serdan ◽  
Gustavo Guevara-Balcazar ◽  
Olga Muñoz-Garcı́a ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Intracellular Calcium ◽  
Aortic Endothelial Cells ◽  
Calcium Kinetics ◽  
Cells In Culture ◽  
Aortic Endothelial

scholarly journals Intracellular calcium ion concentrations in endothelial cells in preeclampsia

2013 ◽  
Vol 1 (2) ◽  
pp. 93-97
Author(s):  
Keiichi Matsubara ◽  
Miki Mori ◽  
Yuko Matsubara ◽  
Yuka Uchikura ◽  
Akihiro Nawa ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Intracellular Calcium ◽  
Calcium Ion ◽  
Ion Concentrations ◽  
Intracellular Calcium Ion

Serotonin uptake by bovine pulmonary artery endothelial cells in culture. II. Stimulation by hypoxia

1986 ◽  
Vol 250 (5) ◽  
pp. C766-C770 ◽  
Author(s):  
S. L. Lee ◽  
B. L. Fanburg
Keyword(s):  
Endothelial Cells ◽  
Pulmonary Artery ◽  
Cell Number ◽  
Morphological Evidence ◽  
Monoamine Oxidase Activity ◽  
Trypan Blue Exclusion ◽  
Cells In Culture ◽  
Low Concentrations ◽  
5 Ht Uptake ◽  
Stimulation Of

Exposure of bovine pulmonary artery endothelial cells to 3% O2 resulted in approximately twofold stimulation of serotonin (5-HT) uptake after 24-48 h when compared with cells exposed to 20% O2. The enhanced uptake was reversed after 48 h when cells were again placed in 20% O2. The stimulation was not observed after 0.5 or 2 h of exposure to hypoxia. The stimulation was present when iproniazid blocked conversion of 5-HT to 5-hydroxyindole-3-acetic acid, indicating that enhanced uptake did not occur through augmentation of monoamine oxidase activity. Stimulation of uptake by hypoxia occurred at low concentrations of 5-HT (up to 10(-6) M) but not at high 5-HT concentrations (greater than 10(-5) M) and was blocked by imipramine or absence of sodium from the medium, indicating that high-affinity transport and not diffusion of 5-HT was stimulated. Furthermore, exposure of cells to hypoxia did not produce morphological evidence of injury or change in protein content or trypan blue exclusion. The cell number of 3% O2-exposed cells was slightly reduced when compared with controls after 48 h. There was no change in cellular ATP or increase in lactate dehydrogenase in medium of cells exposed to 3% O2. Thus exposure of endothelial cells in culture to hypoxia stimulates the membrane activity of 5-HT accumulation with no evidence of injury to the cell.

Secretion of Weibel-Palade bodies observed in extra-alveolar vessels of rabbit lung

1983 ◽  
Vol 54 (5) ◽  
pp. 1284-1286 ◽  
Author(s):  
J. M. McNiff ◽  
J. Gil
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Endothelial Cells ◽  
Pulmonary Artery ◽  
Rabbit Lung ◽  
Vascular Lumen ◽  
Cells In Culture ◽  
Transmission Electron ◽  
Specific Granules

We fixed rabbit lungs by perfusion of osmium into the pulmonary artery and examined in light and transmission electron microscopy a large number of extra-alveolar vessels with a diameter of 0.1–0.25 mm, with emphasis on Weibel-Palade bodies (endothelial specific granules). Weibel-Palade bodies are organelles specific to endothelial cells. Their function is unknown, but they are useful markers for identification of endothelial cells in culture. We were able to observe release of the content of these bodies into the vascular lumen; this indicates that they are secretory.

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