Fluorescent immunolabeling of cancer cells by quantum dots and antibody scFv fragment

2009 ◽  
Vol 14 (2) ◽  
pp. 021004 ◽  
Author(s):  
Tatiana A. Zdobnova ◽  
Sergey G. Dorofeev ◽  
Piter N. Tananaev ◽  
Roman B. Vasiliev ◽  
Taras G. Balandin ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Cancer Cells ◽  
Scfv Fragment

Semiconductor Quantum Dots for Multicolor Fluorescence Imaging and Spectroscopy of Single Cancer Cells

2003 ◽  
Vol 773 ◽  
Author(s):  
Xiaohu Gao ◽  
Shuming Nie ◽  
Wallace H. Coulter
Keyword(s):  
Quantum Dots ◽  
Cancer Cells ◽  
Fluorescence Imaging ◽  
Organic Dyes ◽  
Fluorescent Proteins ◽  
Single Cells ◽  
Quantitative Imaging ◽  
Semiconductor Quantum Dots ◽  
Single Cancer ◽  
Imaging And Spectroscopy

AbstractLuminescent quantum dots (QDs) are emerging as a new class of biological labels with unique properties and applications that are not available from traditional organic dyes and fluorescent proteins. Here we report new developments in using semiconductor quantum dots for quantitative imaging and spectroscopy of single cancer cells. We show that both live and fixed cells can be labeled with multicolor QDs, and that single cells can be analyzed by fluorescence imaging and wavelength-resolved spectroscopy. These results raise new possibilities in cancer imaging, molecular profiling, and disease staging.

scholarly journals Quantum Dots as a Good Carriers of Unsymmetrical Bisacridines for Modulating Cellular Uptake and the Biological Response in Lung and Colon Cancer Cells

Nanomaterials ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 462 ◽  
Author(s):  
Joanna Pilch ◽  
Patrycja Kowalik ◽  
Piotr Bujak ◽  
Anna M. Nowicka ◽  
Ewa Augustin
Keyword(s):  
Quantum Dots ◽  
Cancer Cells ◽  
Cellular Uptake ◽  
Laser Scanning ◽  
Biological Response ◽  
Drug Carriers ◽  
Confocal Laser ◽  
Normal Cells ◽  
H460 Cells ◽  
Hct116 Cells

Nanotechnology-based drug delivery provides a promising area for improving the efficacy of cancer treatments. Therefore, we investigate the potential of using quantum dots (QDs) as drug carriers for antitumor unsymmetrical bisacridine derivatives (UAs) to cancer cells. We examine the influence of QD–UA hybrids on the cellular uptake, internalization (Confocal Laser Scanning Microscope), and the biological response (flow cytometry and light microscopy) in lung H460 and colon HCT116 cancer cells. We show the time-dependent cellular uptake of QD–UA hybrids, which were more efficiently retained inside the cells compared to UAs alone, especially in H460 cells, which could be due to multiple endocytosis pathways. In contrast, in HCT116 cells, the hybrids were taken up only by one endocytosis mechanism. Both UAs and their hybrids induced apoptosis in H460 and HCT116 cells (to a greater extent in H460). Cells which did not die underwent senescence more efficiently following QDs–UAs treatment, compared to UAs alone. Cellular senescence was not observed in HCT116 cells following treatment with both UAs and their hybrids. Importantly, QDgreen/red themselves did not provoke toxic responses in cancer or normal cells. In conclusion, QDs are good candidates for targeted UA delivery carriers to cancer cells while protecting normal cells from toxic drug activities.

scholarly journals Gene Expression and Transcriptome Profiling of Changes in a Cancer Cell Line Post-Exposure to Cadmium Telluride Quantum Dots: Possible Implications in Oncogenesis

Dose-Response ◽  
2021 ◽  
Vol 19 (2) ◽  
pp. 155932582110198
Author(s):  
Mohammed S. Aldughaim ◽  
Mashael R. Al-Anazi ◽  
Marie Fe F. Bohol ◽  
Dilek Colak ◽  
Hani Alothaid ◽  
...  
Keyword(s):  
Gene Expression ◽  
Quantum Dots ◽  
Cancer Cells ◽  
Cadmium Telluride ◽  
Cancer Progression ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Dependent Manner ◽  
Cdte Qds ◽  
Cadmium Telluride Quantum Dots

Cadmium telluride quantum dots (CdTe-QDs) are acquiring great interest in terms of their applications in biomedical sciences. Despite earlier sporadic studies on possible oncogenic roles and anticancer properties of CdTe-QDs, there is limited information regarding the oncogenic potential of CdTe-QDs in cancer progression. Here, we investigated the oncogenic effects of CdTe-QDs on the gene expression profiles of Chang cancer cells. Chang cancer cells were treated with 2 different doses of CdTe-QDs (10 and 25 μg/ml) at different time intervals (6, 12, and 24 h). Functional annotations helped identify the gene expression profile in terms of its biological process, canonical pathways, and gene interaction networks activated. It was found that the gene expression profiles varied in a time and dose-dependent manner. Validation of transcriptional changes of several genes through quantitative PCR showed that several genes upregulated by CdTe-QD exposure were somewhat linked with oncogenesis. CdTe-QD-triggered functional pathways that appear to associate with gene expression, cell proliferation, migration, adhesion, cell-cycle progression, signal transduction, and metabolism. Overall, CdTe-QD exposure led to changes in the gene expression profiles of the Chang cancer cells, highlighting that this nanoparticle can further drive oncogenesis and cancer progression, a finding that indicates the merit of immediate in vivo investigation.

Glycoprotein-based bioimaging of HeLa cancer cells by folate receptor and folate decorated graphene quantum dots

2021 ◽  
pp. 106732
Author(s):  
Jafar Soleymani ◽  
Sajjad Azizi ◽  
Soheil Abbaspour ◽  
Mohammad Hasanzadeh ◽  
Mohammad Hossein Somi ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Cancer Cells ◽  
Folate Receptor ◽  
Graphene Quantum Dots

Fluorescence Imaging of Stem Cells, Cancer Cells and Semi-Thin Sections of Tissues using Silica-Coated CdSe Quantum Dots

2011 ◽  
Vol 21 (4) ◽  
pp. 1365-1370 ◽  
Author(s):  
M. Vibin ◽  
R. Vinayakan ◽  
Annie John ◽  
V. Raji ◽  
C. S. Rejiya ◽  
...  
Keyword(s):  
Stem Cells ◽  
Quantum Dots ◽  
Cancer Cells ◽  
Fluorescence Imaging ◽  
Cdse Quantum Dots ◽  
Thin Sections

Nanobiotechnology and Therapeutics

2018 ◽  
pp. 75-99
Author(s):  
Vikrant
Keyword(s):  
Gene Therapy ◽  
Quantum Dots ◽  
Cancer Cells ◽  
Cancer Detection ◽  
Safety Profile ◽  
Crucial Role ◽  
Target Tissue ◽  
Chemotherapeutic Drugs ◽  
Toxic Potential ◽  
Improved Methods

Nanobiotechnology deals with the application of the tools and processes of nanotechnology to build devices for studying and manipulating biological systems. Current approaches of diagnosis and treatment of various diseases, especially cancer have major limitations such as poor sensitivity or specificity and drug toxicities respectively. Novel and improved methods of cancer detection based on nanoparticles are required to be developed. Some of the nanoparticles used for diagnostic purposes are paramagnetic nanoparticles, quantum dots, nanoshells and nanosomes. Drugs with high toxic potential like cancer chemotherapeutic drugs can be given with a better safety profile with the utility of nanotechnology. These can be made to act specifically at the target tissue by active as well as passive means. Simultaneously, other alternative ways of therapy such as heat induced killings of cancer cells by nanoshells and gene therapy are also being developed. Thus, it indicates that nanomedicine in future would play a crucial role in the treatment of human diseases.

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