Novel time-resolved detector for fluorescence lifetime imaging (Conference Presentation)

2019 ◽  
Author(s):  
Cosimo D'Andrea ◽  
Andrea Farina ◽  
Alessia Candeo ◽  
Alberto Dalla Mora ◽  
Rudi Lussana ◽  
...  
Keyword(s):  
Fluorescence Lifetime ◽  
Fluorescence Lifetime Imaging ◽  
Time Resolved ◽  
Lifetime Imaging

scholarly journals The biochemical resolving power of fluorescence lifetime imaging

2021 ◽  
Author(s):  
Andrew L. Trinh ◽  
Alessandro Esposito
Keyword(s):  
Fluorescence Lifetime ◽  
Resolving Power ◽  
Fluorescence Lifetime Imaging ◽  
Time Resolved ◽  
Instrument Response Function ◽  
Lifetime Imaging ◽  
Use Of Time ◽  
Single Living Cells ◽  
Microscopy Techniques

AbstractA deeper understanding of spatial resolution in microscopy fostered a technological revolution that is now permitting us to investigate the structure of the cell with nanometer resolution. Although fluorescence microscopy techniques enable scientists to investigate both the structure and biochemistry of the cell, the biochemical resolving power of a microscope is a physical quantity that is not well-defined or studied. To overcome this limitation, we carried out a theoretical investigation of the biochemical resolving power in fluorescence lifetime imaging microscopy, one of the most effective tools to investigate biochemistry in single living cells. With the theoretical analysis of information theory and Monte Carlo simulations, we describe how the ‘biochemical resolving power’ in time-resolved sensing depends on instrument specifications. We unravel common misunderstandings on the role of the instrument response function and provide theoretical insights that have significant practical implications in the design and use of time-resolved instrumentation.

A Time-Resolved Four-Tap Lock-In Pixel CMOS Image Sensor for Real-Time Fluorescence Lifetime Imaging Microscopy

2018 ◽  
Vol 53 (8) ◽  
pp. 2319-2330 ◽  
Author(s):  
Min-Woong Seo ◽  
Yuya Shirakawa ◽  
Yoshimasa Kawata ◽  
Keiichiro Kagawa ◽  
Keita Yasutomi ◽  
...  
Keyword(s):  
Real Time ◽  
Fluorescence Lifetime ◽  
Cmos Image Sensor ◽  
Image Sensor ◽  
Fluorescence Lifetime Imaging Microscopy ◽  
Fluorescence Lifetime Imaging ◽  
Time Resolved ◽  
Lifetime Imaging ◽  
Lock In

scholarly journals Synthesis, Photophysics, and Solvatochromic Studies of an Aggregated-Induced-Emission Luminogen Useful in Bioimaging

Sensors ◽  
2019 ◽  
Vol 19 (22) ◽  
pp. 4932 ◽  
Author(s):  
Laura Espinar-Barranco ◽  
Marta Meazza ◽  
Azahara Linares-Perez ◽  
Ramon Rios ◽  
Jose Manuel Paredes ◽  
...  
Keyword(s):  
Fluorescence Lifetime ◽  
Fluorescence Emission ◽  
Fluorescence Lifetime Imaging ◽  
Selective Isolation ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
Lifetime Imaging ◽  
Intracellular Compartments ◽  
Photophysical Behavior ◽  
Heterogeneous Matrix

Biological samples are a complex and heterogeneous matrix where different macromolecules with different physicochemical parameters cohabit in reduced spaces. The introduction of fluorophores into these samples, such as in the interior of cells, can produce changes in the fluorescence emission properties of these dyes, caused by the specific physicochemical properties of cells. This effect can be especially intense with solvatofluorochromic dyes, where changes in the polarity environment surrounding the dye can drastically change the fluorescence emission. In this article, we studied the photophysical behavior of a new dye and confirmed the aggregation-induced emission (AIE) phenomenon with different approaches, such as by using different solvent proportions, increasing the viscosity, forming micelles, and adding bovine serum albumin (BSA), through analysis of the absorption and steady-state and time-resolved fluorescence. Our results show the preferences of the dye for nonpolar media, exhibiting AIE under specific conditions through immobilization. Additionally, this approach offers the possibility of easily determining the critical micelle concentration (CMC). Finally, we studied the rate of spontaneous incorporation of the dye into cells by fluorescence lifetime imaging and observed the intracellular pattern produced by the AIE. Interestingly, different intracellular compartments present strong differences in fluorescence intensity and fluorescence lifetime. We used this difference to isolate different intracellular regions to selectively study these regions. Interestingly, the fluorescence lifetime shows a strong difference in different intracellular compartments, facilitating selective isolation for a detailed study of specific organelles.

scholarly journals Compressive hyperspectral time-resolved wide-field fluorescence lifetime imaging

2017 ◽  
Vol 11 (7) ◽  
pp. 411-414 ◽  
Author(s):  
Qi Pian ◽  
Ruoyang Yao ◽  
Nattawut Sinsuebphon ◽  
Xavier Intes
Keyword(s):  
Fluorescence Lifetime ◽  
Wide Field ◽  
Fluorescence Lifetime Imaging ◽  
Time Resolved ◽  
Lifetime Imaging

scholarly journals A CTRW-based model of time-resolved fluorescence lifetime imaging in a turbid medium

2010 ◽  
Vol 283 (23) ◽  
pp. 4832-4839 ◽  
Author(s):  
Victor Chernomordik ◽  
Amir H. Gandjbakhche ◽  
Moinuddin Hassan ◽  
Sinisa Pajevic ◽  
George H. Weiss
Keyword(s):  
Fluorescence Lifetime ◽  
Turbid Medium ◽  
Fluorescence Lifetime Imaging ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
Lifetime Imaging

Analytical solutions for time-resolved fluorescence lifetime imaging in a turbid medium such as tissue: errata

2003 ◽  
Vol 20 (9) ◽  
pp. 1833 ◽  
Author(s):  
David Hattery ◽  
Victor Chernomordik ◽  
Murray Loew ◽  
Israel Gannot ◽  
Amir Gandjbakhche
Keyword(s):  
Fluorescence Lifetime ◽  
Analytical Solutions ◽  
Turbid Medium ◽  
Fluorescence Lifetime Imaging ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
Lifetime Imaging

Polyfluorenes with Phosphorescent Iridium(III) Complexes for Time-Resolved Luminescent Biosensing and Fluorescence Lifetime Imaging

2016 ◽  
Vol 26 (36) ◽  
pp. 6505-6505 ◽  
Author(s):  
Huifang Shi ◽  
Huibin Sun ◽  
Huiran Yang ◽  
Shujuan Liu ◽  
Gareth Jenkins ◽  
...  
Keyword(s):  
Fluorescence Lifetime ◽  
Fluorescence Lifetime Imaging ◽  
Time Resolved ◽  
Lifetime Imaging
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