Based on the land use data of 2000, 2010 and 2020, using GIS technology along with landscape ecology methods, this paper monitored the changes in land use and landscape pattern in Wuhan. The findings are as follows: (1) the main features of land use change in Wuhan were the expansion of urban area and the decline of cropland, forest, wetland and water in recent 20 years; (2) forest, wetland and water kept a transfer-out trend while urban kept a transfer-in trend; (3) the fragmentation degree of forest, grassland and urban landscapes decreased from 2000 to 2020; (4) the patch shapes of almost all landscapes tended to be more regular under the human interventions. It is thereby worth reducing the interference intensity of human activities on landscape pattern in the process of urban growth.