Effect of Whey Protein Hydrolysate on Performance and Recovery of Top-Class Orienteering Runners

This trial aimed to examine the effect of whey protein hydrolysate intake before and after exercise sessions on endurance performance and recovery in elite orienteers during a training camp. Eighteen elite orienteers participated in a randomized controlled intervention trial during a 1-week training camp (13 exercise sessions). Half of the runners (PRO-CHO) ingested a protein drink before (0.3 g kg−1) and a protein-carbohydrate drink after (0.3 g protein kg−1 and 1 g carbohydrate kg−1) each exercise session. The others ingested energy and timematched carbohydrate drinks (CHO). A 4-km run-test with 20 control points was performed before and on the last day of the intervention. Blood and saliva were obtained in the mornings, before and after run-tests, and after the last training session. During the intervention, questionnaires were fulfilled regarding psychological sense of performance capacity and motivation. PRO-CHO and not CHO improved performance in the 4-km run-test (interaction p < .05). An increase in serum creatine kinase was observed during the week, which was greater in CHO than PRO-CHO (interactionp < .01). Lactate dehydrogenase (p < .001) and cortisol (p = .057) increased during the week, but the change did not differ between groups. Reduction in sense of performance capacity during the intervention was greater in CHO (p < .05) than PRO-CHO. In conclusion, ingestion of whey protein hydrolysate before and after each exercise session improves performance and reduces markers of muscle damage during a strenuous 1-week training camp. The results indicate that protein supplementation in conjunction with each exercise session facilitates the recovery from strenuous training in elite orienteers.

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Soluble Whey Protein Hydrolysate Ameliorates Muscle Atrophy Induced by Immobilization via Regulating the PI3K/Akt Pathway in C57BL/6 Mice

Nutrients ◽

10.3390/nu12113362 ◽

2020 ◽

Vol 12 (11) ◽

pp. 3362

Author(s):

Ji Eun Shin ◽

Seok Jun Park ◽

Seung Il Ahn ◽

Se-Young Choung

Keyword(s):

Skeletal Muscle ◽

Whey Protein ◽

Muscle Mass ◽

Muscle Atrophy ◽

Skeletal Muscle Mass ◽

Protein Hydrolysate ◽

Protein Hydrolysates ◽

Protein Supplementation ◽

Whey Protein Hydrolysates ◽

Whey Protein Hydrolysate

Sarcopenia, a loss of skeletal muscle mass and function, is prevalent in older people and associated with functional decline and mortality. Protein supplementation is necessary to maintain skeletal muscle mass and whey protein hydrolysates have the best nutrient quality among food proteins. In the first study, C57BL/6 mice were subjected to immobilization for 1 week to induce muscle atrophy. Then, mice were administered with four different whey protein hydrolysates for 2 weeks with continuous immobilization. Among them, soluble whey protein hydrolysate (WP-S) had the greatest increase in grip strength, muscle weight, and cross-sectional area of muscle fiber than other whey protein hydrolysates. To investigate the molecular mechanism, we conducted another experiment with the same experimental design. WP-S significantly promoted the phosphoinositide 3-kinase (PI3K)/protein kinase B (Akt)/mammalian target of rapamycin (mTOR) pathway and inhibited the PI3K/Akt/forkhead box O (FoxO) pathway. In addition, it increased myosin heavy chain (MyHC) expression in both the soleus and quadriceps and changed MyHC isoform expressions. In conclusion, WP-S attenuated muscle atrophy induced by immobilization by enhancing the net protein content regulating muscle protein synthesis and degradation. Thus, it is a necessary and probable candidate for developing functional food to prevent sarcopenia.

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Supplement With Whey Protein Hydrolysate in Contrast to Carbohydrate Supports Mitochondrial Adaptations in Trained Runners

10.21203/rs.3.rs-36706/v1 ◽

2020 ◽

Author(s):

Mette Hansen ◽

Mikkel Oxfeldt ◽

Anne E. Larsen ◽

Lise S. Thomsen ◽

Rokkedal-Lausch Torben ◽

...

Keyword(s):

Cytochrome C ◽

Endurance Training ◽

Whey Protein ◽

Protein Hydrolysate ◽

Mitochondrial Proteins ◽

Exercise Session ◽

Post Intervention ◽

And Performance ◽

Whey Protein Hydrolysate ◽

Trained Runners

Abstract Background: Protein supplementation has been suggested to augment endurance training adaptations by increasing mixed muscle and myofibrillar protein synthesis and lean body mass. However, a potential beneficial effect on mitochondrial adaptations is yet to be clarified.The aim of the present study was to investigate the effect of consuming whey protein hydrolysate before and whey protein hydrolysate plus carbohydrate (PRO-CHO) after each exercise session during a six-week training period compared to similarly timed intake of isocaloric CHO supplements on biomarkers of mitochondrial biogenesis, VO2max and performance in trained runners.Methods: Twenty-four trained runners (VO2max 60.73.7 ml O2 kg-1 min1) completed a six-week block randomized controlled intervention period, consisting of progressive running training. Subjects were randomly assigned to either PRO-CHO or CHO and matched in pairs for gender, age, VO2max, training and performance status. The PRO-CHO group ingested a protein beverage (0.3g kg-1) before and protein-carbohydrate beverage (0.3g protein kg-1 and 1g carbohydrate kg-1) after each exercise session. The CHO group ingested an energy matched carbohydrate beverage. Resting muscle biopsies obtained pre and post intervention were analyzed for mitochondrial specific enzyme activity and mitochondrial protein content. Subjects completed a 6K time trial (6K TT) and a VO2max test pre, midway (only 6K TT) and post intervention. Results: Following six weeks of endurance training Cytochrome C (Cyt C) protein content was significantly higher in the PRO-CHO group compared to the CHO group (p<0.05), with several other mitochondrial proteins (Succinate dehydrogenase (SDHA), Cytochrome C oxidase (COX-IV), Voltage-dependent anion channel (VDAC), Heat shock protein 60 (HSP60), and Prohibitin (PHB1)) following a similar, but non-significant pattern (p=0.07-0.14). β-hydroxyacyl-CoA dehydrogenase (HAD) activity was significantly lower after training in the CHO group (p<0.01), but not in the PRO-CHO group (p=0.24). VO2max and 6K TT was significantly improved after training with no significant difference between groups.Conclusion: Intake of whey PRO hydrolysate before and whey PRO hydrolysate plus CHO after each exercise session during a six-week endurance training period may augment training effects on specific mitochondrial proteins compared to intake of iso-caloric CHO but does not alter VO2max or 6K TT performance.Trial registration: clinicaltrials.gov, NCT03561337. Registered 6 June 2018 – Retrospectively registered.

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Supplement with whey protein hydrolysate in contrast to carbohydrate supports mitochondrial adaptations in trained runners

10.21203/rs.3.rs-36706/v2 ◽

2020 ◽

Author(s):

Mette Hansen ◽

Mikkel Oxfeldt ◽

Anne E. Larsen ◽

Lise S. Thomsen ◽

Rokkedal-Lausch Torben ◽

...

Keyword(s):

Cytochrome C ◽

Endurance Training ◽

Whey Protein ◽

Protein Hydrolysate ◽

Mitochondrial Proteins ◽

Exercise Session ◽

Post Intervention ◽

And Performance ◽

Whey Protein Hydrolysate ◽

Trained Runners

Abstract Background: Protein supplementation has been suggested to augment endurance training adaptations by increasing mixed muscle and myofibrillar protein synthesis and lean body mass. However, a potential beneficial effect on mitochondrial adaptations is yet to be clarified.The aim of the present study was to investigate the effect of consuming whey protein hydrolysate before and whey protein hydrolysate plus carbohydrate (PRO-CHO) after each exercise session during a six-week training period compared to similarly timed intake of isocaloric CHO supplements on biomarkers of mitochondrial biogenesis, VO2max and performance in trained runners.Methods: Twenty-four trained runners (VO2max 60.7±3.7 ml O2 kg-1 min1) completed a six-week block randomized controlled intervention period, consisting of progressive running training. Subjects were randomly assigned to either PRO-CHO or CHO and matched in pairs for gender, age, VO2max, training and performance status. The PRO-CHO group ingested a protein beverage (0.3g kg-1) before and protein-carbohydrate beverage (0.3g protein kg-1 and 1g carbohydrate kg-1) after each exercise session. The CHO group ingested an energy matched carbohydrate beverage. Resting muscle biopsies obtained pre and post intervention were analyzed for mitochondrial specific enzyme activity and mitochondrial protein content. Subjects completed a 6K time trial (6K TT) and a VO2max test pre, midway (only 6K TT) and post intervention. Results: Following six weeks of endurance training Cytochrome C (Cyt C) protein content was significantly higher in the PRO-CHO group compared to the CHO group (p<0.05), with several other mitochondrial proteins (Succinate dehydrogenase (SDHA), Cytochrome C oxidase (COX-IV), Voltage-dependent anion channel (VDAC), Heat shock protein 60 (HSP60), and Prohibitin (PHB1)) following a similar, but non-significant pattern (p=0.07-0.14). β-hydroxyacyl-CoA dehydrogenase (HAD) activity was significantly lower after training in the CHO group (p<0.01), but not in the PRO-CHO group (p=0.24). VO2max and 6K TT was significantly improved after training with no significant difference between groups.Conclusion: Intake of whey PRO hydrolysate before and whey PRO hydrolysate plus CHO after each exercise session during a six-week endurance training period may augment training effects on specific mitochondrial proteins compared to intake of iso-caloric CHO but does not alter VO2max or 6K TT performance.

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Supplement with whey protein hydrolysate in contrast to carbohydrate supports mitochondrial adaptations in trained runners

Journal of the International Society of Sports Nutrition ◽

10.1186/s12970-020-00376-3 ◽

2020 ◽

Vol 17 (1) ◽

Author(s):

Mette Hansen ◽

Mikkel Oxfeldt ◽

Anne E. Larsen ◽

Lise S. Thomsen ◽

Torben Rokkedal-Lausch ◽

...

Keyword(s):

Cytochrome C ◽

Endurance Training ◽

Whey Protein ◽

Protein Hydrolysate ◽

Mitochondrial Proteins ◽

Exercise Session ◽

Post Intervention ◽

And Performance ◽

Whey Protein Hydrolysate ◽

Trained Runners

Abstract Background Protein supplementation has been suggested to augment endurance training adaptations by increasing mixed muscle and myofibrillar protein synthesis and lean body mass. However, a potential beneficial effect on mitochondrial adaptations is yet to be clarified. The aim of the present study was to investigate the effect of consuming whey protein hydrolysate before and whey protein hydrolysate plus carbohydrate (PRO-CHO) after each exercise session during a six-week training period compared to similarly timed intake of isocaloric CHO supplements on biomarkers of mitochondrial biogenesis, VO2max and performance in trained runners. Methods Twenty-four trained runners (VO2max 60.7 ± 3.7 ml O2 kg− 1 min1) completed a six-week block randomized controlled intervention period, consisting of progressive running training. Subjects were randomly assigned to either PRO-CHO or CHO and matched in pairs for gender, age, VO2max, training and performance status. The PRO-CHO group ingested a protein beverage (0.3 g kg− 1) before and protein-carbohydrate beverage (0.3 g protein kg− 1 and 1 g carbohydrate kg− 1) after each exercise session. The CHO group ingested an energy matched carbohydrate beverage. Resting muscle biopsies obtained pre and post intervention were analyzed for mitochondrial specific enzyme activity and mitochondrial protein content. Subjects completed a 6 K time trial (6 K TT) and a VO2max test pre, midway (only 6 K TT) and post intervention. Results Following six weeks of endurance training Cytochrome C (Cyt C) protein content was significantly higher in the PRO-CHO group compared to the CHO group (p < 0.05), with several other mitochondrial proteins (Succinate dehydrogenase (SDHA), Cytochrome C oxidase (COX-IV), Voltage-dependent anion channel (VDAC), Heat shock protein 60 (HSP60), and Prohibitin (PHB1)) following a similar, but non-significant pattern (p = 0.07–0.14). β-hydroxyacyl-CoA dehydrogenase (HAD) activity was significantly lower after training in the CHO group (p < 0.01), but not in the PRO-CHO group (p = 0.24). VO2max and 6 K TT was significantly improved after training with no significant difference between groups. Conclusion Intake of whey PRO hydrolysate before and whey PRO hydrolysate plus CHO after each exercise session during a six-week endurance training period may augment training effects on specific mitochondrial proteins compared to intake of iso-caloric CHO but does not alter VO2max or 6 K TT performance. Trial registration clinicaltrials.gov, NCT03561337. Registered 6 June 2018 – Retrospectively registered.

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Preparation and characterization of whey protein hydrolysate-Zn complexes

Journal of Food Measurement & Characterization ◽

10.1007/s11694-019-00287-1 ◽

2019 ◽

Vol 14 (1) ◽

pp. 254-261 ◽

Cited By ~ 2

Author(s):

Rongchun Wang ◽

Shenghua He ◽

Yifan Xuan ◽

Cuilin Cheng

Keyword(s):

Whey Protein ◽

Protein Hydrolysate ◽

Whey Protein Hydrolysate

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Correction: Whey Protein Hydrolysate Enhances HSP90 but Does Not Alter HSP60 and HSP25 in Skeletal Muscle of Rats

PLoS ONE ◽

10.1371/annotation/0cb4a83a-2b26-410f-bdda-ff62dae25eea ◽

2014 ◽

Vol 9 (1) ◽

Cited By ~ 5

Author(s):

Carolina Soares Moura ◽

Pablo Christiano Barboza Lollo ◽

Priscila Neder Morato ◽

Luciana Hisayama Nisishima ◽

Everardo MagalhÃ£es Carneiro ◽

...

Keyword(s):

Skeletal Muscle ◽

Whey Protein ◽

Protein Hydrolysate ◽

Whey Protein Hydrolysate

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IMPACT OF FORTIFYING COW'S MILK WITH WHEY PROTEIN HYDROLYSATE ON YOGHURT QUALITY

Menoufia Journal of Food and Dairy Sciences ◽

10.21608/mjfds.2020.171450 ◽

2020 ◽

Vol 5 (5) ◽

pp. 65-77

Author(s):

K. M. K. Kebary ◽

S. A. Husien ◽

R. M. Badawi ◽

M. A. M. Habib

Keyword(s):

Whey Protein ◽

Protein Hydrolysate ◽

Cow’S Milk ◽

Cow's Milk ◽

Whey Protein Hydrolysate

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Co-ingestion of whey protein hydrolysate with milk minerals rich in calcium potently stimulates glucagon-like peptide-1 secretion: an RCT in healthy adults

European Journal of Nutrition ◽

10.1007/s00394-019-02092-4 ◽

2019 ◽

Vol 59 (6) ◽

pp. 2449-2462 ◽

Cited By ~ 1

Author(s):

Yung-Chih Chen ◽

Harry A. Smith ◽

Aaron Hengist ◽

Oliver J. Chrzanowski-Smith ◽

Ulla Ramer Mikkelsen ◽

...

Keyword(s):

Whey Protein ◽

Protein Hydrolysate ◽

Healthy Adults ◽

Mean Difference ◽

High Dose ◽

Calcium Citrate ◽

Double Blind ◽

Gut Hormone ◽

Whey Protein Hydrolysate ◽

Milk Minerals

Abstract Purpose To examine whether calcium type and co-ingestion with protein alter gut hormone availability. Methods Healthy adults aged 26 ± 7 years (mean ± SD) completed three randomized, double-blind, crossover studies. In all studies, arterialized blood was sampled postprandially over 120 min to determine GLP-1, GIP and PYY responses, alongside appetite ratings, energy expenditure and blood pressure. In study 1 (n = 20), three treatments matched for total calcium content (1058 mg) were compared: calcium citrate (CALCITR); milk minerals rich in calcium (MILK MINERALS); and milk minerals rich in calcium plus co-ingestion of 50 g whey protein hydrolysate (MILK MINERALS + PROTEIN). In study 2 (n = 6), 50 g whey protein hydrolysate (PROTEIN) was compared to MILK MINERALS + PROTEIN. In study 3 (n = 6), MILK MINERALS was compared to the vehicle of ingestion (water plus sucralose; CONTROL). Results MILK MINERALS + PROTEIN increased GLP-1 incremental area under the curve (iAUC) by ~ ninefold (43.7 ± 11.1 pmol L−1 120 min; p < 0.001) versus both CALCITR and MILK MINERALS, with no difference detected between CALCITR (6.6 ± 3.7 pmol L−1 120 min) and MILK MINERALS (5.3 ± 3.5 pmol L−1 120 min; p > 0.999). MILK MINERALS + PROTEIN produced a GLP-1 iAUC ~ 25% greater than PROTEIN (p = 0.024; mean difference: 9.1 ± 6.9 pmol L−1 120 min), whereas the difference between MILK MINERALS versus CONTROL was small and non-significant (p = 0.098; mean difference: 4.2 ± 5.1 pmol L−1 120 min). Conclusions When ingested alone, milk minerals rich in calcium do not increase GLP-1 secretion compared to calcium citrate. Co-ingesting high-dose whey protein hydrolysate with milk minerals rich in calcium increases postprandial GLP-1 concentrations to some of the highest physiological levels ever reported. Registered at ClinicalTrials.gov: NCT03232034, NCT03370484, NCT03370497.

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