scholarly journals VIM-2-Producing Pseudomonas spp. in Uruguay: Sequence Types, Pulsotypes, and Class 1 Integrons Including New Variable Regions FeaturingblaVIM-2andblaGES-7

2016 ◽  
Vol 60 (9) ◽  
pp. 5620-5622 ◽  
Author(s):  
Romina Papa Ezdra ◽  
Inés Bado ◽  
Nicolás Cordeiro ◽  
Claudia Gutierrez ◽  
Patricia Hitateguy ◽  
...  
Keyword(s):  
Variable Regions ◽  
Class 1 Integrons ◽  
Pseudomonas Spp ◽  
Class 1 ◽  
Sequence Types

scholarly journals Complex Class 1 Integrons with Diverse Variable Regions, Including aac(6′)-Ib-cr, and a Novel Allele, qnrB10, Associated with ISCR1 in Clinical Enterobacterial Isolates from Argentina

2007 ◽  
Vol 51 (12) ◽  
pp. 4466-4470 ◽  
Author(s):  
María Paula Quiroga ◽  
Patricia Andres ◽  
Alejandro Petroni ◽  
Alfonso J. C. Soler Bistué ◽  
Leonor Guerriero ◽  
...  
Keyword(s):  
Variable Region ◽  
Quinolone Resistance ◽  
The Novel ◽  
Complex Class ◽  
Unique Region ◽  
Variable Regions ◽  
Class 1 Integrons ◽  
Class 1 ◽  
Novel Allele

ABSTRACT Transferable quinolone resistance has not previously been reported in Argentina. Here we describe three complex class 1 integrons harboring the novel allele qnrB10 in a unique region downstream of orf513, one of them also containing aac(6′)-Ib-cr within the variable region of integrons. The three arrays differed from bla CTX-M-2-bearing integrons, which are broadly distributed in Argentina.

Novel class 1 Integrons and sequence types in VIM-2 and VIM-11-producing clinical strains of Enterobacter cloacae

2017 ◽  
Vol 54 ◽  
pp. 374-378 ◽  
Author(s):  
D. De Belder ◽  
D. Faccone ◽  
N. Tijet ◽  
R.G. Melano ◽  
M. Rapoport ◽  
...  
Keyword(s):  
Enterobacter Cloacae ◽  
Clinical Strains ◽  
Class 1 Integrons ◽  
Class 1 ◽  
Sequence Types

scholarly journals Antimicrobial Resistance and Spread of Class 1 Integrons among Salmonella Serotypes

2000 ◽  
Vol 44 (8) ◽  
pp. 2166-2169 ◽  
Author(s):  
Beatriz Guerra ◽  
Sara Soto ◽  
Santiago Cal ◽  
M. Carmen Mendoza
Keyword(s):  
Multidrug Resistance ◽  
Resistance Genes ◽  
Antimicrobial Agents ◽  
Resistance Pattern ◽  
Variable Regions ◽  
Class 1 Integrons ◽  
Class 1 ◽  
Salmonella Serotypes ◽  
Reference Strains ◽  
Spanish Region

ABSTRACT The resistance profiles, for 15 antimicrobial agents, of 333Salmonella strains representing the most frequent nontyphoidal serotypes, isolated between 1989 and 1998 in a Spanish region, and 9 reference strains were analyzed. All strains were susceptible to amikacin, ceftazidime, ciprofloxacin, and imipenem, and 31% were susceptible to all antimicrobials tested. The most frequent types of resistance were to sulfadiazine, tetracycline, streptomycin, spectinomycin, ampicillin, and chloramphenicol (ranging from 46 to 22%); 13% were resistant to these six drugs. This multidrug resistance pattern was found alone or together with other resistance types within serotypes Typhimurium (45%), Panama (23%), and Virchow (4%). Each isolate was also screened for the presence of class 1 integrons and selected resistance genes therein; seven variable regions which carried one (aadA1a, aadA2, orpse-1) or two (dfrA14-aadA1a,dfrA1-aadA1a, oxa1-aadA1a, orsat1-aadA1a) resistance genes were found in integrons.

INTEGRON DIVERSITY IN BLAVIM-2-CARRYING CARBAPENEM-RESISTANT CLINICAL PSEUDOMONAS AERUGINOSA ISOLATES

2019 ◽  
Vol 64 (8) ◽  
pp. 497-502 ◽  
Author(s):  
T. A. Savinova ◽  
Yu. A. Bocharova ◽  
A. V. Lazareva ◽  
I. V. Chebotar ◽  
N. A. Mayanskiy
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Gene Cassette ◽  
Variable Regions ◽  
Class 1 Integrons ◽  
Carbapenem Resistant ◽  
Class 1 ◽  
Encoding Gene ◽  
Clonal Spread ◽  
Pathogen Populations

The growing prevalence of metallo-β-lactamase (MBL)-producing Pseudomonas aeruginosa in nosocomial pathogen populations has been attributed to their clonal spread, and/or horizontal transfer of MBL determinants in mobile genetic elements, including integrons. To characterize the genetic background of the beta-lactamase VIM-2 encoding gene in the population of carbapenem-resistant (Carba-R) P. aeruginosa clinical isolates.The detection of class 1 integrons was performed by PCR. Typing of the class 1 integrons containing the blaVIM gene cassette was performed by the PCR-restriction fragment length polymorphism (RFLP) approach followed by sequencing of variable regions of class 1 integrons. Five types of the blaVIM-2-carrying integrons were identified: ST654-isolates accounting for more than 50% of the Carba-R population harbored In56; ST235-isolates contained In559 (26% Carba-R isolates); ST111-isolates (19% Carba-R isolates) were characterized by carrying In59-like integron; two ST235-isolates harbored In59 and In249 each. Except In56, carrying the only blaVIM-2-gene cassette, all other identified integron types harbored the genes of resistance to trimethoprim and/or aminoglycosides. No new types of integrons were identified in the P. aeruginosa clinical isolates. The observed correlation of the integron type with specific STs indicates a clonal dissemination of significant resistance determinant producers - ST111, ST654 and ST235 epidemic lines. The features of the integron variable regions can be used for the epidemiological characterization of clinical P. aeruginosa isolates.

scholarly journals Emergence of OXA-carbapenemase- and 16S rRNA methylase-producing international clones of Acinetobacter baumannii in Norway

2011 ◽  
Vol 60 (4) ◽  
pp. 515-521 ◽  
Author(s):  
Nabil Karah ◽  
Bjørg Haldorsen ◽  
Nils O. Hermansen ◽  
Yngvar Tveten ◽  
Eivind Ragnhildstveit ◽  
...  
Keyword(s):  
16S Rrna ◽  
Acinetobacter Baumannii ◽  
Sequence Group ◽  
Aminoglycoside Resistance ◽  
Genotypic Resistance ◽  
Variable Regions ◽  
Class 1 Integrons ◽  
Class 1 ◽  
Pcr Assays ◽  
16S Rrna Methylase

This study was designed to investigate the molecular epidemiology and antibiotic-resistance characteristics of 11 carbapenem-resistant clinical isolates of Acinetobacter baumannii obtained in Norway between 2004 and 2009. Interestingly, all the isolates were linked with recent hospitalization outside Norway. The epidemiological status was investigated by multilocus sequence typing (MLST), multiplex PCR assays for major international clones, typing of bla OXA-51-like variants and PFGE. The genotypic-resistance characteristics, including the occurrence of OXA-carbapenemase-encoding and 16S rRNA methylase-encoding genes and class 1 integrons, were investigated by PCR assays and sequencing. Seven isolates were found to harbour bla OXA-66 and belong to MLST clonal complexes (CCs) CC2P (Pasteur Institute scheme) and CC92B (Bartual scheme), and international clone II. One isolate harboured bla OXA-69, and belonged to CC1P, CC109B and international clone I. Two isolates belonged to sequence group 9, probably a subgroup of international clone I, and one isolate belonged to sequence group 4, a proposed novel international clone. All isolates contained an acquired OXA-carbapenemase-encoding gene: bla OXA-23-like (n=9), bla OXA-24-like (n=1) and bla OXA-58-like (n=1). Four isolates with high-level aminoglycoside-resistance contained the 16S rRNA methylase-encoding armA gene. Class 1 integrons with six different variable regions were detected. Sequence analysis of gene cassettes identified four aminoglycoside (aacA4, aac(6′)-Im, aadA1 and aacC1), two chloramphenicol (catB8 and cm1A5), one β-lactamase (bla OXA-20) and one rifampicin (arr-2) resistance gene in various combinations. In conclusion, the occurrence of A. baumannii isolates producing OXA carbapenemase and 16S rRNA methylase in Norway was related to the worldwide distribution of international clones I and II, and the emergence of novel international clones.

Antimicrobial Resistance and Presence of Class 1 Integrons Among Different Serotypes of Salmonella spp. Recovered From Children with Diarrhea in Tehran, Iran

2020 ◽  
Vol 20 (2) ◽  
pp. 160-166
Author(s):  
Seyedeh Hanieh Eshaghi Zadeh ◽  
Hossein Fahimi ◽  
Fatemeh Fardsanei ◽  
Mohammad Mehdi Soltan Dallal
Keyword(s):  
Antibiotic Resistance ◽  
Antimicrobial Resistance ◽  
Multidrug Resistant ◽  
Salmonella Spp ◽  
Class 1 Integron ◽  
Class 1 Integrons ◽  
Food Borne ◽  
Class 1 ◽  
Resistance Patterns ◽  
C 30

Background: Salmonellosis is a major food-borne disease worldwide. The increasing prevalence of antimicrobial resistance among food-borne pathogens such as Salmonella spp. is concerning. Objective: The main objective of this study is to identify class 1 integron genes and to determine antibiotic resistance patterns among Salmonella isolates from children with diarrhea. Methods: A total of 30 Salmonella isolates were recovered from children with diarrhea. The isolates were characterized for antimicrobial susceptibility and screened for the presence of class 1 integron genes (i.e. intI1, sulI1, and qacEΔ1). Results: The most prevalent serotype was Enteritidis 36.7%, followed by Paratyphi C (30%), and Typhimurium (16.7%). The highest rates of antibiotic resistance were obtained for nalidixic acid (53.3%), followed by streptomycin (40%), and tetracycline (36.7%). Regarding class 1 integrons, 36.7%, 26.7%, and 33.3% of the isolates carried intI1, SulI, and qacEΔ1, respectively, most of which (81.8%) were multidrug-resistant (MDR). Statistical analysis revealed that the presence of class 1 integron was significantly associated with resistance to streptomycin and tetracycline (p = 0.042). However, there was no association between class 1 integron and other antibiotics used in this study (p > 0.05). Conclusion: The high frequency of integron class 1 gene in MDR Salmonella strains indicates that these mobile genetic elements are versatile among different Salmonella serotypes, and associated with reduced susceptibility to many antimicrobials.

scholarly journals Multidrug Resistance Dissemination in Escherichia coli Isolated from Wild Animals: Bacterial Clones and Plasmid Complicity

2021 ◽  
Vol 12 (1) ◽  
pp. 123-137
Author(s):  
Carolina Sabença ◽  
Gilberto Igrejas ◽  
Patrícia Poeta ◽  
Frédéric Robin ◽  
Richard Bonnet ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Epidemiological Data ◽  
Generation Cephalosporin ◽  
Wild Animals ◽  
Variable Regions ◽  
E Coli ◽  
Wild Fauna ◽  
Long Read ◽  
Sequence Types ◽  
Animal Isolates

Objectives. Epidemiological data concerning third-generation cephalosporin (3GC) resistance in wild fauna are scarce. The aim of this study was to characterize the resistance genes, their genetic context, and clonal relatedness in 17 Escherichia coli resistant to 3GC isolated from wild animals. Methods. The isolates were characterized by short-read whole genome sequencing, and long-read sequencing was used for the hybrid assembly of plasmid sequences. Results. The 3GC resistance gene most identified in the isolates was the extended-spectrum β-lactamases (ESBL)-encoding gene blaCTX-M-1 (82.3%), followed by blaCTX-M-32 (5.9%), blaCTX-M-14 (5.9%), and blaSHV-12 (5.9%). E. coli isolates mainly belonged to the sequence types (STs) rarely reported from humans. The single nucleotide polymorphism (SNP)-based typing showed that most E. coli genomes from wild animals (wild boars, birds of prey, and buzzards) formed clonal clusters (<5 SNPs), showing a clonal dissemination crossing species boundaries. blaCTX-M-1-harboring IncI1-ST3 plasmid was the predominant ESBL-encoding plasmid (76.4%) in wild animal isolates. Plasmid comparison revealed a 110-kb self-transferable plasmid consisting of a conserved backbone and two variable regions involved in antimicrobial resistance and in interaction with recipient cells during conjugation. Conclusion. Our results highlighted the unexpected clonal dissemination of blaCTX-M-1-encoding clones and the complicity of IncI1-ST3 plasmid in the spread of blaCTX-M-1 within wild fauna.

Sign in / Sign up

Export Citation Format

Share Document