scholarly journals Prevalence of First-Step Mutants among Levofloxacin-Susceptible Invasive Isolates of Streptococcus pneumoniae in the United States

2006 ◽  
Vol 50 (4) ◽  
pp. 1561-1563 ◽  
Author(s):  
Mathias W. R. Pletz ◽  
Ardaman P. Shergill ◽  
Lesley McGee ◽  
Bernard Beall ◽  
Cynthia G. Whitney ◽  
...  
Keyword(s):  
United States ◽  
Nursing Home ◽  
Streptococcus Pneumoniae ◽  
Restriction Fragment Length Polymorphism ◽  
Length Polymorphism ◽  
Fragment Length Polymorphism ◽  
Nursing Home Residents ◽  
The United States ◽  
Fluoroquinolone Resistance ◽  
Restriction Fragment Length

ABSTRACT By use of a PCR-restriction fragment length polymorphism assay, we screened 496 levofloxacin-susceptible invasive pneumococcal strains (MIC ≤ 2 mg/liter) for quinolone resistance-determining region mutations known to confer fluoroquinolone resistance. Among those with a levofloxacin MIC of 2 mg/liter, 16.2% of isolates recovered from nursing home residents and 6.4% from non-nursing home residents had first-step mutations.

scholarly journals PCR-Restriction Fragment Length Polymorphism Assay for Detection of gyrA Mutations Associated with Fluoroquinolone Resistance in Campylobacter coli

2004 ◽  
Vol 48 (12) ◽  
pp. 4886-4888 ◽  
Author(s):  
Rodrigo Alonso ◽  
Estibaliz Mateo ◽  
Cecilia Girbau ◽  
Estibaliz Churruca ◽  
Irati Martinez ◽  
...  
Keyword(s):  
Restriction Fragment Length Polymorphism ◽  
Gene Product ◽  
Point Mutation ◽  
Restriction Fragment ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Fluoroquinolone Resistance ◽  
Campylobacter Coli ◽  
Restriction Fragment Length

ABSTRACT A fragment of the gyrA gene was sequenced from 34 isolates of Campylobacter coli, including 23 isolates resistant to ciprofloxacin. All ciprofloxacin-resistant isolates examined by DNA sequencing carried a point mutation at position Thr-86 on the gyrA gene product, involving the replacement of Thr-86 by Ile. A combined PCR-restriction fragment length polymorphism technique using RsaI was developed to detect this mutation.

scholarly journals Computerized Analysis of Restriction Fragment Length Polymorphism Patterns: Comparative Evaluation of Two Commercial Software Packages

1998 ◽  
Vol 36 (5) ◽  
pp. 1318-1323 ◽  
Author(s):  
P. Gerner-Smidt ◽  
L. M. Graves ◽  
Susan Hunter ◽  
B. Swaminathan
Keyword(s):  
Restriction Fragment Length Polymorphism ◽  
Restriction Fragment ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Molecular Size ◽  
The United States ◽  
Reference Pattern ◽  
Two Systems ◽  
Restriction Fragment Length

Two computerized restriction fragment length polymorphism pattern analysis systems, the BioImage system and the GelCompar system (Molecular Analyst Fingerprinting Plus in the United States), were compared. The two systems use different approaches to compare patterns from different gels. In GelCompar, a standard reference pattern in one gel is used to normalize subsequent gels containing lanes with the same reference pattern. In BioImage, the molecular sizes of the fragments are calculated from size standards present in each gel. The molecular size estimates obtained with the two systems for 12 restriction fragments of phage λ were between 97 and 101% of their actual sizes, with a standard deviation of less than 1% of the average estimated size for most fragments. At the window sizes used for analysis, the GelCompar system performed somewhat better than BioImage in identifying visually identical patterns generated by electrophoretic separation ofHhaI-restricted DNA of Listeria monocytogenes. Both systems require the user to make critical decisions in the analysis. It is very important to visually verify that the systems are finding all bands in each lane and that no artifacts are being detected; both systems allow manual editing. It is also important to verify results obtained in the pattern matching or clustering portions of the analysis.

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