scholarly journals Inhibitory Effect of Biocides on the Viable Masses and Matrices of Staphylococcus aureus and Pseudomonas aeruginosa Biofilms

2010 ◽  
Vol 76 (10) ◽  
pp. 3135-3142 ◽  
Author(s):  
K. Toté ◽  
T. Horemans ◽  
D. Vanden Berghe ◽  
L. Maes ◽  
P. Cos
Keyword(s):  
Hydrogen Peroxide ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Sodium Hypochlorite ◽  
Peracetic Acid ◽  
Inhibitory Effect ◽  
European Guidelines ◽  
The Matrix ◽  
Antibiofilm Agents

ABSTRACT Bacteria and matrix are essential for the development of biofilms, and assays should therefore target both components. The current European guidelines for biocidal efficacy testing are not adequate for sessile microorganisms; hence, alternative discriminatory test protocols should be used. The activities of a broad range of biocides on Staphylococcus aureus and Pseudomonas aeruginosa biofilms were evaluated using such in vitro assays. Nearly all selected biocides showed a significant decrease in S. aureus biofilm viability, with sodium hypochlorite and peracetic acid as the most active biocides. Only hydrogen peroxide and sodium hypochlorite showed some inhibitory effect on the matrix. Treatment of P. aeruginosa biofilms was roughly comparable to that of S. aureus biofilms. Peracetic acid was the most active on viable mass within 1 min of contact. Isopropanol ensured a greater than 99.999% reduction of P. aeruginosa viability after at least 30 min of contact. Comparable to results with S. aureus, sodium hypochlorite and hydrogen peroxide markedly reduced the P. aeruginosa matrix. This study clearly demonstrated that despite their aspecific mechanisms of action, most biocides were active only against biofilm bacteria, leaving the matrix undisturbed. Only hydrogen peroxide and sodium hypochlorite were active on both the biofilm matrix and the viable mass, making them the better antibiofilm agents. In addition, this study emphasizes the need for updated and standardized guidelines for biofilm susceptibility testing of biocides.

Evaluation of a Sporicidal Peracetic Acid/Hydrogen Peroxide–Based Daily Disinfectant Cleaner

2014 ◽  
Vol 35 (11) ◽  
pp. 1414-1416 ◽  
Author(s):  
Abhishek Deshpande ◽  
Thriveen S. C. Mana ◽  
Jennifer L. Cadnum ◽  
Annette C. Jencson ◽  
Brett Sitzlar ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Staphylococcus Aureus ◽  
Clostridium Difficile ◽  
Sodium Hypochlorite ◽  
Peracetic Acid ◽  
Infect Control Hosp ◽  
Organic Load ◽  
Methicillin Resistant

OxyCide Daily Disinfectant Cleaner, a novel peracetic acid/hydrogen peroxide–based sporicidal disinfectant, was as effective as sodium hypochlorite for in vitro killing of Clostridium difficile spores, methicillin-resistant Staphylococcus aureus, and vancomcyin-resistant enterococci. OxyCide was minimally affected by organic load and was effective in reducing pathogen contamination in isolation roomsInfect Control Hosp Epidemiol 2014;35(11):1414–1416

scholarly journals Resistance of Pseudomonas aeruginosa to Liquid Disinfectants on Contaminated Surfaces before Formation of Biofilms

2000 ◽  
Vol 83 (6) ◽  
pp. 1415-1422 ◽  
Author(s):  
Jose-Luis Sagripanti ◽  
Aylin Bonifacino
Keyword(s):  
Hydrogen Peroxide ◽  
Pseudomonas Aeruginosa ◽  
Sodium Hypochlorite ◽  
Peracetic Acid ◽  
Biofilm Formation ◽  
Step Method ◽  
Quantitative Test ◽  
Viable Bacteria ◽  
One Step ◽  
Respective Product

Abstract A comparison was made of the effectiveness of popular disinfectants (Cavicide, Cidexplus, Clorox, Exspor, Lysol, Renalin, and Wavicide) under conditions prescribed for disinfection in the respective product labels on Pseudomonas aeruginosa either in suspension or deposited onto surfaces of metallic or polymeric plastic devices. The testing also included 7 nonformulated germicidal agents (glutaraldehyde, formaldehyde, peracetic acid, hydrogen peroxide, sodium hypochlorite, phenol, and cupric ascorbate) commonly used in disinfection and decontamination. Results showed that P. aeruginosa is on average 300-fold more resistant when present on contaminated surfaces than in suspension. This increase in resistance agrees with results reported in studies of biofilms, but unexpectedly, it precedes biofilm formation. The surface to which bacteria are attached can influence the effectiveness of disinfectants. Viable bacteria attached to devices may require dislodging through more than a one-step method for detection. The data, obtained with a sensitive and quantitative test, suggest that disinfectants are less effective on contaminated surfaces than generally acknowledged.

scholarly journals Hydrogen Peroxide-Mediated Interference Competition by Streptococcus pneumoniae Has No Significant Effect on Staphylococcus aureus Nasal Colonization of Neonatal Rats

2008 ◽  
Vol 191 (2) ◽  
pp. 571-575 ◽  
Author(s):  
Elisa Margolis
Keyword(s):  
Hydrogen Peroxide ◽  
Staphylococcus Aureus ◽  
Streptococcus Pneumoniae ◽  
Interference Competition ◽  
Inhibitory Effect ◽  
Nasal Colonization ◽  
Neonatal Rats ◽  
Mixed Inoculum ◽  
Relative Scarcity

ABSTRACT It has been proposed that the relative scarcity of Staphylococcus aureus and Streptococcus pneumoniae cocolonization in the nasopharynxes of humans can be attributed to hydrogen peroxide-mediated interference competition. Previously it has been shown in vitro that H2O2 produced by S. pneumoniae is bactericidal to S. aureus. To ascertain whether H2O2 has this inhibitory effect in the nasal passages of neonatal rats, colonization experiments were performed with S. aureus and S. pneumoniae. The results of these experiments with neonatal rats are inconsistent with the hypothesis that hydrogen peroxide-mediated killing of S. aureus by S. pneumoniae is responsible for the relative scarcity of cocolonization by these bacteria. In mixed-inoculum colonization experiments and experiments where S. aureus invaded the nasopharynxes of rats with established S. pneumoniae populations, the density of S. aureus did not differ whether the S. pneumoniae strain was H2O2 secreting or non-H2O2 secreting (SpxB). Moreover, the advantage of catalase production by S. aureus in competition with a non-catalase-producing strain (KatA) during nasal colonization was no greater in the presence of H2O2-producing S. pneumoniae than in the presence of non-H2O2-producing S. pneumoniae.

Effect of Thio- and Hydrazino-derivatives of Uracil, 6-Azauracil and 6-Azathymine on the Growth of Some Microorganisms in Vitro

1972 ◽  
Vol 27 (7) ◽  
pp. 818-821 ◽  
Author(s):  
Alexander Spassov ◽  
Evgeny Golovinsky ◽  
Nadejda Spassovska ◽  
Liliana Maneva
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Bacillus Subtilis ◽  
Neurospora Crassa ◽  
Candida Tropicalis ◽  
Inhibitory Effect ◽  
Streptococcus Faecalis ◽  
Derivatives Of

The antibacterial activity of 2-thiouracil, 2-methylthiouracil, 2-hydrazinouracil, 2,4-dithiouracil, 2-thio-4-hydrazinouracil, 2-thio-6-azathymine, 2-hydrazino-6-azathymine, 2,4-dithio-6-azathymine, 2-thio-4-hydrazino-6-azathymine, 2,4-dimethylthio-6-azathymine, 2-methylthio-4-hydrazino-6-azathymine, 4-thio-6-azauracil and 4-hydrazino-6-azauracil has been studied on the growth of: Staphylococcus aureus 209, Streptococcus faecalis 775, Escherichiia coli 387, Pseudomonas aeruginosa, Bacillus subtilis, Candida tropicalis and Neurospora crassa 9863.The highest inhibitory effect was observed with 4-hydrazino-derivatives of 2-thiouracil, 6-azauracil and 2-thio-6-azathymine.

scholarly journals Study of the inhibitory effect of Quercus Coccifera's aqueous extract on Staphylococcus aureus and Pseudomonas aeruginosa In vitro

2014 ◽  
Vol 10 (11) ◽  
pp. 689-692 ◽  
Author(s):  
Arezo Judaki ◽  
◽  
Jafar Panahi ◽  
Mohamad Reza Havasian ◽  
Parnian Tajbakhsh ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Aqueous Extract ◽  
Inhibitory Effect

scholarly journals IN VITRO INHIBITORY EFFECT OF ALCOHOLIC AND AQUEOUS EXTRACT OF UMBILICUS INTERMEDIUS BOISS (NAFE VENUS) ON STAPHYLOCOCCUS AUREUS AND PSEUDOMONAS AERUGINOSA

2014 ◽  
Vol 3 (31) ◽  
pp. 8686-8691
Author(s):  
Mohamad Ali Roozegar ◽  
Zeynab Pournazari ◽  
Mohamad Reza Nazari ◽  
Mohamad Reza Havasian ◽  
Jafar Panahi ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Aqueous Extract ◽  
Inhibitory Effect

scholarly journals The Effect of Three Different Disinfectant Materials on Polyether Impressions by Spray Method

2012 ◽  
Vol 3 (3) ◽  
pp. 229-233
Author(s):  
Ehsan Ghasemi ◽  
Hamid Badrian ◽  
Nafiseh Hosseini ◽  
Navid Khalighinejad
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Candida Albicans ◽  
Sodium Hypochlorite ◽  
P Value ◽  
Plastic Bags ◽  
Spray Method ◽  
Significant Difference ◽  
Different Types

ABSTRACT Background Dentists, dental equipments and dental laboratories are exposed to different types of pathogenic microorganisms. The aim of this study is to investigate the effect of three different types of disinfectant agents: Sodium hypochlorite 0.525%, Epimax and Deconex, on polyether impressions after 5 and 10 minutes. Materials and methods In this in vitro experimental study, 66 circular samples of polyether impression material of 1 cm diameter and 2 mm thickness were contaminated with Staphylococcus aureus (ATCC29213) Pseudomonas aeruginosa (ATCC27853) and Candida albicans fungus (PTCC5027). Except for control samples, all of them were disinfected with sodium hypochlorite 0.525, Deconex and Epimax by way of spraying. Afterward, they kept in plastic bags with humid rolled cotton for 5 and 10 minutes. In order to isolate bacteria, the samples were immersed in 2% trypsin for 1 hour and then the solution was diluted with normal salin in portion of 1, 1/2, and 1/4. The trypsin suspensions were transferred to culture plates and the number of colonies was counted after 24 and 48 hours for bacteria and after 72 hours for fungus. For data analysis Mann-Whitney statistical test was used (α = 0.05). Results Epimax and sodium hypochlorite thoroughly eliminated Candida albicans as time elapsed from 5 to 10 minutes; however, the other two microorganisms were not eliminated completely. Deconex was completely efficient for all microorganisms when immersion time was enhanced from 5 to 10 minutes. There was a significant difference between efficacy of Deconex-sodium hypochlorite and sodium hypochlorite-Epimax for Pseudomonas aeruginosa in 5-minute immersion (p-value:0/046) Moreover, Deconex and sodium hypochlorite were significantly different for Staphylococcus aureus (p-value:0/046) and Pseudomonas aeruginosa in 10-minute immersion (p < 0.05). Conclusion According to the results of this study, none of the three disinfection materials could eradicate three different kinds of microorganisms in 5 minutes, but in 10 minutes, Deconex could completely eradicate all microorganisms compared to other disinfection agents, which is a good indicator for high efficacy of this agent in disinfecting polyether impressions. How to cite this article Ghasemi E, Badrian H, Hosseini N, Khalighinejad N. The Effect of Three Different Disinfectant Materials on Polyether Impressions by Spray Method. World J Dent 2012;3(3):229-233.

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