scholarly journals Characterization of Strains unlike Mesorhizobium loti That Nodulate Lotus spp. in Saline Soils of Granada, Spain

2010 ◽  
Vol 76 (12) ◽  
pp. 4019-4026 ◽  
Author(s):  
María J. Lorite ◽  
Socorro Muñoz ◽  
José Olivares ◽  
María J. Soto ◽  
Juan Sanjuán
Keyword(s):  
Gene Sequence ◽  
Low Fertility ◽  
Salt Tolerant ◽  
Forage Legumes ◽  
Sequence Analyses ◽  
Mesorhizobium Loti ◽  
Mesorhizobium Tianshanense ◽  
High Salt Tolerance ◽  
Repetitive Extragenic Palindromic Pcr

ABSTRACT Lotus species are forage legumes with potential as pastures in low-fertility and environmentally constrained soils, owing to their high persistence and yield under those conditions. The aim of this work was the characterization of phenetic and genetic diversity of salt-tolerant bacteria able to establish efficient symbiosis with Lotus spp. A total of 180 isolates able to nodulate Lotus corniculatus and Lotus tenuis from two locations in Granada, Spain, were characterized. Molecular identification of the isolates was performed by repetitive extragenic palindromic PCR (REP-PCR) and 16S rRNA, atpD, and recA gene sequence analyses, showing the presence of bacteria related to different species of the genus Mesorhizobium: Mesorhizobium tarimense/Mesorhizobium tianshanense, Mesorhizobium chacoense/Mesorhizobium albiziae, and the recently described species, Mesorhizobium alhagi. No Mesorhizobium loti-like bacteria were found, although most isolates carried nodC and nifH symbiotic genes closely related to those of M. loti, considered the type species of bacteria nodulating Lotus, and other Lotus rhizobia. A significant portion of the isolates showed both high salt tolerance and good symbiotic performance with L. corniculatus, and many behaved like salt-dependent bacteria, showing faster growth and better symbiotic performance when media were supplemented with Na or Ca salts.

scholarly journals Molecular characterization of Eurytrema coelomaticum in cattle from Paraná, Brazil

2014 ◽  
Vol 23 (3) ◽  
pp. 383-386 ◽  
Author(s):  
Gustavo Freire Figueira ◽  
Victor Henrique Silva de Oliveira ◽  
Alessandra Taroda ◽  
Amauri Alcindo Alfieri ◽  
Selwyn Arlington Headley
Keyword(s):  
Molecular Characterization ◽  
Gene Sequence ◽  
Domestic Animals ◽  
18S Rrna Gene ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Sequence Analyses ◽  
Pcr Assay ◽  
Bovine Pancreas

This study investigated the occurrence of Eurytremaspp. in cattle by analysis of the partial 18S rRNA gene sequence. Trematodes from 44 bovine pancreas were collected and classified based on typical morphological features. PCR assay and sequence analyses of amplified products confirmed that the trematodes classified as Eurytrema coelomaticum were phylogenetically distinct from those identified as E. pancreaticum. The results of this study represent the first molecular characterization of E. coelomaticum within the Americas, and provide an efficient method to differentiate digenean trematodes of domestic animals.

scholarly journals Identification of strains Bacillus aerophilus MTCC 7304T as Bacillus altitudinis and Bacillus stratosphericus MTCC 7305T as a Proteus sp. and the status of the species Bacillus aerius Shivaji et al. 2006. Request for an Opinion

2015 ◽  
Vol 65 (Pt_9) ◽  
pp. 3228-3231 ◽  
Author(s):  
Yang Liu ◽  
N. Ramesh Kumar ◽  
Qiliang Lai ◽  
Juan Du ◽  
Anatoly P. Dobritsa ◽  
...  
Keyword(s):  
Gene Sequence ◽  
Pulsed Field Gel Electrophoresis ◽  
Phenotypic Traits ◽  
Sequence Analyses ◽  
Bacillus Altitudinis ◽  
The Status ◽  
Type Strains ◽  
Respective Species ◽  
Rejected Names

On the basis of 16S rRNA, rpoB, gyrB and pycA gene sequence analyses, characterization of biochemical features and other phenotypic traits and pulsed-field gel electrophoresis (PFGE) fingerprinting, it was ascertained that strains Bacillus aerius MTCC 7303T, Bacillus aerophilus MTCC 7304T and Bacillus stratosphericus MTCC 7305T do not conform to the descriptions of the type strains of the respective species. Strains MTCC 7303T and MTCC 7304T were indistinguishable from Bacillus altitudinis DSM 21631T, while strain MTCC 7305T should be classified as a representative of a Proteus sp. Our attempts to find other deposits of the type strains of these species were unsuccessful. Therefore, the results support the Request for an Opinion on the status of the species Bacillus aerophilus and Bacillus stratosphericus by Branquinho et al. [Branquinho, R., Klein, G., Kämpfer, P. & Peixe, L. V. (2015). Int J Syst Evol Microbiol 65, 1101]. It is also proposed that the Judicial Commission should place the name Bacillus aerius on the list of rejected names if a suitable replacement type strain cannot be found or a neotype is not proposed within two years following the publication of this Request (Rule 18c).

Isolation and characterization of silicate mineral-solubilizing Bacillus globisporus Q12 from the surfaces of weathered feldspar

2008 ◽  
Vol 54 (12) ◽  
pp. 1064-1068 ◽  
Author(s):  
Xia Fang Sheng ◽  
Fei Zhao ◽  
Lin Yan He ◽  
Gang Qiu ◽  
Liang Chen
Keyword(s):  
Gene Sequence ◽  
Active Agent ◽  
Salt Tolerant ◽  
Silicate Mineral ◽  
16S Rdna Gene ◽  
Silicate Minerals ◽  
Isolation And Characterization ◽  
Gene Sequence Analysis ◽  
Acetic Acids

A silicate mineral-solubilizing bacterial strain Q12 was isolated from the surfaces of weathered feldspar and identified as Bacillus globisporus Q12 based on the 16S rDNA gene sequence analysis. Three silicate minerals (feldspar, muscovite, and biotite) were used to investigate potassium and silicon mobilization by strain Q12. In liquid cultures, the strain showed better growth on the biotite than on feldspar and muscovite. The biotite was the best potassium source for growth of the strain. Solubilization of potassium and silicon from the silicate minerals by the strain resulted mostly from the action of organic acids. Gluconic acid seemed to be the most active agent for the solubilization of the 3 silicate minerals. Gluconic and acetic acids were likely involved in the solubilization of feldspar. The strain could be acid or alkali and salt tolerant and temperature resistant.

scholarly journals Molecular Characterization of Bovine Enteric Caliciviruses: a Distinct Third Genogroup of Noroviruses (Norwalk-Like Viruses) Unlikely To Be of Risk to Humans

2003 ◽  
Vol 77 (4) ◽  
pp. 2789-2798 ◽  
Author(s):  
S. L. Oliver ◽  
A. M. Dastjerdi ◽  
S. Wong ◽  
L. El-Attar ◽  
C. Gallimore ◽  
...  
Keyword(s):  
Gene Sequence ◽  
Orf3 Gene ◽  
Sequence Analyses ◽  
Animal Reservoir ◽  
Reading Frame ◽  
Reverse Transcription Pcr ◽  
The United Kingdom ◽  
Enteric Caliciviruses ◽  
Relationship Of

ABSTRACT Bovine enteric caliciviruses (BoCVs) have been classified in the Norovirus (Norwalk-like virus) genus of the Caliciviridae, raising questions about zoonotic transmission and an animal reservoir for the human Norwalk-like viruses (NLVs), an important cause of nonbacterial gastroenteritis in humans. We examined the genetic relationship of human NLVs to BoCVs that were identified by using reverse transcription-PCR with primer pairs originally designed to detect human NLVs. Polymerase, capsid, and open reading frame 3 (ORF3) gene sequence analyses of BoCVs that were identified from 1976 to 2000 from throughout the United Kingdom showed that BoCVs formed a distinct third genogroup of closely related viruses distinct from the human genogroup I and II NLVs. Evidence was not obtained to support the concept that BoCVs are circulating in humans and pose a threat to human health.

scholarly journals Characterization of a Salt-Tolerant Family 42 β-Galactosidase from a Psychrophilic AntarcticPlanococcus Isolate

2000 ◽  
Vol 66 (6) ◽  
pp. 2438-2444 ◽  
Author(s):  
Peter P. Sheridan ◽  
Jean E. Brenchley
Keyword(s):  
Gene Sequence ◽  
Rrna Gene ◽  
Salt Tolerant ◽  
Ice Shelf ◽  
Gene Encoding ◽  
Reporter Enzyme ◽  
Optimum Ph ◽  
Metal Requirement ◽  
The 16S Rrna Gene

ABSTRACT We isolated a gram-positive, halotolerant psychrophile from a hypersaline pond located on the McMurdo Ice Shelf in Antarctica. A phylogenetic analysis of the 16S rRNA gene sequence of this organism showed that it is a member of the genus Planococcus. This assignment is consistent with the morphology and physiological characteristics of the organism. A gene encoding a β-galactosidase in this isolate was cloned in an Escherichia coli host. Sequence analysis of this gene placed it in glycosidase family 42 most closely related to an enzyme from Bacillus circulans. Even though an increasing number of family 42 glycosidase sequences are appearing in databases, little information about the biochemical features of these enzymes is available. Therefore, we purified and characterized this enzyme. The purified enzyme did not appear to have any metal requirement, had an optimum pH of 6.5 and an optimum temperature of activity at 42°C, and was irreversibly inactivated within 10 min when it was incubated at 55°C. The enzyme had an apparent Km of 4.9 μmol ofo-nitrophenyl-β-d-galactopyranoside, and theV max was 467 μmol ofo-nitrophenol produced/min/mg of protein at 39°C. Of special interest was the finding that the enzyme remained active at high salt concentrations, which makes it a possible reporter enzyme for halotolerant and halophilic organisms.

scholarly journals Taxonomic characterization of nine strains isolated from clinical and environmental specimens, and proposal of Corynebacterium tuberculostearicum sp. nov.

2004 ◽  
Vol 54 (4) ◽  
pp. 1055-1061 ◽  
Author(s):  
Carole Feurer ◽  
Dominique Clermont ◽  
François Bimet ◽  
Adina Candréa ◽  
Mary Jackson ◽  
...  
Keyword(s):  
Gene Sequence ◽  
Fatty Acid Content ◽  
Food Samples ◽  
Cell Protein ◽  
Rrna Gene ◽  
Biochemical Tests ◽  
Taxonomic Analysis ◽  
Gene Sequence Analysis ◽  
Taxonomic Characterization

Nine unidentified Gram-positive, lipophilic corynebacteria were isolated from clinical and food samples and subjected to a polyphasic taxonomic analysis. The bacteria were distinguished from Corynebacterium species with validly published names by biochemical tests, fatty acid content and whole-cell protein analysis. Comparative 16S rRNA gene sequence analysis demonstrated unambiguously that the nine strains were related phylogenetically to the species ‘Corynebacterium tuberculostearicum’ and represented a distinct subline within the genus Corynebacterium. On the basis of both phenotypic and phylogenetic evidence, the formal description of Corynebacterium tuberculostearicum sp. nov. is proposed. The type strain of C. tuberculostearicum is Medalle XT (=LDC-20T=CIP 107291T=CCUG 45418T=ATCC 35529T).

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