Characterization of Listeria monocytogenes isolated from poultry products and from the poultry-processing environment by random amplification of polymorphic DNA and multilocus enzyme electrophoresis.

1995 ◽  
Vol 61 (6) ◽  
pp. 2139-2144 ◽  
Author(s):  
L M Lawrence ◽  
A Gilmour
Keyword(s):  
Listeria Monocytogenes ◽  
Enzyme Electrophoresis ◽  
Multilocus Enzyme Electrophoresis ◽  
Poultry Products ◽  
Random Amplification ◽  
Poultry Processing

Application of Multilocus Enzyme Electrophoresis to Epidemiologic Investigations ofXanthomonas Maltophilia

1991 ◽  
Vol 12 (3) ◽  
pp. 163-167 ◽  
Author(s):  
Barbara Schable ◽  
Margarita E. Villarino ◽  
Martin S. Favero ◽  
J. Michael Miller
Keyword(s):  
Intensive Care Unit ◽  
Intensive Care ◽  
Epidemiological Data ◽  
Case Definition ◽  
Enzyme Electrophoresis ◽  
Typing Method ◽  
Multilocus Enzyme Electrophoresis ◽  
General Community ◽  
Suspected Outbreak

AbstractObjective:To test the utility of a newly developed multilocus enzyme electrophoresis typing method for Xanthomonas maltophilia.Design:Isolates were first screened by slide agglutination, which served as the standard to characterize the outbreak strains. All isolates were then subjected to multilocus enzyme elec-trophoresis and the results analyzed based on epidemiological data.Setting:This outbreak occurred in a shock-trauma intensive care unit of a large general community hospital.Patients:Patients admitted to the shock-trauma intensive care unit who had X maltophilia isolated from any site > 24 hours after admission met the case definition. Specimens from patients who fit the case definition were characterized, as were specimens from other patients that were used as controls for nonoutbreak isolates. Environmental samples were also evaluated for X maltophilia.Results:Most of the 64 isolates received during this outbreak were serotype 10, and when they were subjected to multilocus enzyme electro-phoresis, one electrophoretic type predominated and correlated to most outbreak isolates. Unrelated isolates of serotype 10 from other institutions all exhibited unique electrophoretic types.Conclusion:Application of multilocus enzyme electrophoresis to X maltophilia outbreaks is a valuable addition to the characterization of suspected outbreak strains.

scholarly journals Characterization of Urease-Positive Thermophilic Campylobacter Subspecies by Multilocus Enzyme Electrophoresis Typing

2003 ◽  
Vol 69 (6) ◽  
pp. 3308-3310 ◽  
Author(s):  
Motoo Matsuda ◽  
Aki Kaneko ◽  
Timothy Stanley ◽  
B. Cherie Millar ◽  
M. Miyajima ◽  
...  
Keyword(s):  
Allelic Variation ◽  
Enzyme Electrophoresis ◽  
Multilocus Enzyme Electrophoresis ◽  
Thermophilic Campylobacter ◽  
Variation Data ◽  
Quantitative Analyses ◽  
The Mean ◽  
Campylobacter Lari ◽  
Reference Strains

ABSTRACT Thirty-one urease-positive thermophilic Campylobacter (UPTC) isolates, including three reference strains (NCTC12892, NCTC12895 and NCTC12896), and three Campylobacter lari isolates, which were isolated from several countries and sources, were compared genotypically by using multilocus enzyme electrophoresis (MLEE). We examined allelic variation around seven enzyme loci, including the adenylate kinase, alkaline phosphatase, catalase, fumarase, malic enzyme, malate dehydrogenase, and l-phenylalanyl-l-leucine peptidase loci. MLEE typing revealed the presence of 23 different electrophoretic types (ETs) among the 31 UPTC isolates, and 14 isolates shared six electrophoretic profiles. Three different ETs were identified for the three C. lari isolates examined, and no ETs were shared by UPTC and C. lari isolates. Quantitative analyses were subsequently performed by using allelic variation data, and the results demonstrated that the mean genetic diversity was 0.655. In conclusion, MLEE demonstrated that the UPTC isolates examined are genetically hypervariable and form a cluster separate from the C. lari cluster.

Comparison of ribotyping and multilocus enzyme electrophoresis for subtyping of Listeria monocytogenes isolates.

1994 ◽  
Vol 32 (12) ◽  
pp. 2936-2943 ◽  
Author(s):  
L M Graves ◽  
B Swaminathan ◽  
M W Reeves ◽  
S B Hunter ◽  
R E Weaver ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Enzyme Electrophoresis ◽  
Multilocus Enzyme Electrophoresis
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