scholarly journals Use of Taenia crassiceps Cysticercus Antigen Preparations for Detection of Antibodies in Cerebrospinal Fluid Samples from Patients with Neurocysticercosis (Taenia solium)

2002 ◽  
Vol 9 (1) ◽  
pp. 190-193 ◽  
Author(s):  
Alessandra Xavier Pardini ◽  
Regina Helena Peralta ◽  
Adelaide José Vaz ◽  
Luis dos Ramos Machado ◽  
José Mauro Peralta
Keyword(s):  
Cerebrospinal Fluid ◽  
Affinity Chromatography ◽  
Molecular Mass ◽  
Sensitivity And Specificity ◽  
Concanavalin A ◽  
Taenia Solium ◽  
Enzyme Linked Immunosorbent Assay ◽  
Good Reproducibility ◽  
Taenia Crassiceps ◽  
Glycoprotein Antigen

ABSTRACT Antigen extracts obtained from the vesicular fluid of Taenia crassiceps cysticerci and from fractions purified by affinity chromatography with the lectin concanavalin A and the glycoprotein antigen separated by electrophoresis were used for the detection of Taenia solium anticysticercus antibodies. The sensitivity and specificity obtained for all antigens were 100% in enzyme-linked immunosorbent assay with good reproducibility. Using immunoblotting of the three antigens, low-molecular-mass peptides (18 and 14 kDa) were characterized only in cerebrospinal fluid samples from patients with neurocysticercosis. The results confirm that antigen fractions purified from T. crassiceps cisticerci are important sources of specific peptides and proved to be efficient in detecting anti-T. solium antibodies.

scholarly journals Neurocysticercosis: detection of IgG, IgA and IgE antibodies in cerebrospinal fluid, serum and saliva samples by ELISA with Taenia solium and Taenia crassiceps antigens

2000 ◽  
Vol 58 (1) ◽  
pp. 18-24 ◽  
Author(s):  
EDNÉIA CASAGRANDA BUENO ◽  
ADELAIDE JOSÉ VAZ ◽  
LUÍS DOS RAMOS MACHADO ◽  
JOSÉ ANTONIO LIVRAMENTO
Keyword(s):  
Cerebrospinal Fluid ◽  
Sensitivity And Specificity ◽  
Taenia Solium ◽  
Control Group ◽  
Ige Antibodies ◽  
Taenia Crassiceps ◽  
Inactive Form

We assayed samples of cerebrospinal fluid (CSF), serum and saliva from patients with neurocysticercoses, control group and individuals with other parasitoses, by ELISA with Taenia crassiceps vesicular fluid antigen (Tcra) and Taenia solium total antigen (Tso) for the detection of antibodies. The sensitivity for IgG-Tcra was 100% for CSF and serum, and 32.0% for saliva; and for IgG-Tso 100% for CSF, 80.0% for serum and 24.% for saliva. Specificity was 100% for CSF and 80.0% for serum with both antigens, and 100% for saliva with Tcra and 87.5% with Tso. The sensitivity and specificity for IgA-Tcra was, respectively, 40.0% and 100% for CSF, 36.0% and 97.1% for serum, and 4.0% and 90.0% for saliva. IgE detection showed 24.0% sensitivity and 97.1% specificity for serum, with no detection in CSF samples. The search for antibodies revealed the presence of IgG > IgA > IgE in CSF, serum and saliva samples, with IgG being present in all phases of the disease, while IgA/IgE were more frequent in the inactive form.

scholarly journals Taenia saginata Metacestode Antigenic Fractions without Affinity to Concanavalin A Are an Important Source of Specific Antigens for the Diagnosis of Human Neurocysticercosis

2010 ◽  
Vol 17 (4) ◽  
pp. 638-644 ◽  
Author(s):  
Heliana B. Oliveira ◽  
Gleyce A. Machado ◽  
José R. Mineo ◽  
Julia M. Costa-Cruz
Keyword(s):  
Sensitivity And Specificity ◽  
Concanavalin A ◽  
Taenia Solium ◽  
Specific Antigen ◽  
Enzyme Linked Immunosorbent Assay ◽  
Taenia Saginata ◽  
Serum Samples ◽  
Unbound Fraction ◽  
Immunoblot Assay ◽  
Homologous Antigen

ABSTRACT Taenia saginata metacestode antigens have been constituted a useful alternative antigen for neurocysticercosis (NC) serodiagnosis, particularly due to an increasing difficulty to obtain Taenia solium homologous antigen. Cross-reactivity with Echinococcus granulosus infection occurs in homologous and heterologous antigens and could be avoided by using different purified methods. The present study evaluated antigen fractions obtained from saline extracts of T. saginata metacestodes purified by affinity chromatography with jacalin or concanavalin A (ConA) lectins to detect IgG antibodies by enzyme-linked immunosorbent assay (ELISA) and immunoblot analysis to diagnose human NC. Serum samples were collected from 142 individuals: 40 of them were diagnosed with NC, 62 presented Taenia sp. and other parasites, and 40 were apparently healthy individuals. The jacalin- and ConA-unbound fractions demonstrated sensitivity and specificity higher than those of bound fractions. Among unbound fractions, ConA demonstrated statistically higher sensitivity and specificity by ELISA (90% and 93.1%, respectively). By immunoblot assay, the 64- to 68-kDa component from the ConA-unbound fraction showed 100% sensitivity and specificity, making this component suitable for use as a specific antigen for diagnosis of NC. To our knowledge, this is the first report showing the relevance of using the unbound ConA fraction of T. saginata metacestodes to diagnose NC. In conclusion, the results obtained herein clearly demonstrate that antigenic fractions without affinity to ConA, obtained from T. saginata metacestodes, are an important source of specific peptides and are efficient in the diagnosis of NC when tested by immunoblot assay.

scholarly journals Evaluation of Taenia solium and Taenia crassiceps cysticercal antigens for immunodiagnosis of neurocysticercosis using ELISA on cerebrospinal fluid samples

2007 ◽  
Vol 40 (2) ◽  
pp. 152-155 ◽  
Author(s):  
Lisandra Akemi Suzuki ◽  
Gisele Cristina Arruda ◽  
Elizabeth Maria Aparecida Barasnevicius Quagliato ◽  
Qláudio Lúcio Rossi
Keyword(s):  
Cerebrospinal Fluid ◽  
Sensitivity And Specificity ◽  
Neurological Disorders ◽  
Taenia Solium ◽  
Igg Antibodies ◽  
Taenia Crassiceps ◽  
Specific Antibodies ◽  
Immunological Reactions

The efficacy of whole parasite and vesicular fluid antigen extracts from Taenia solium and Taenia crassiceps cysticerci for immunodiagnosis of neurocysticercosis was evaluated using ELISA on cerebrospinal fluid samples. Anticysticercal IgG antibodies were assayed in cerebrospinal fluid samples from 23 patients with neurocysticercosis and 35 patients with other neurological disorders. The ELISA reaction for the whole Taenia solium cysticercal extract showed 91.3% sensitivity and 94.3% specificity, whereas the sensitivity and specificity of the ELISA for the whole Taenia crassiceps cysticercal extract were 87% and 94.3%, respectively. The ELISA reactions for vesicular fluid from Taenia solium or Taenia crassiceps showed 91.3% sensitivity and 97.1% specificity. Considering the results obtained from the four antigen preparations, vesicular fluid from Taenia solium and Taenia crassiceps cysticerci may be useful as a source of antigens for immunological reactions that are used for detecting specific antibodies in cerebrospinal fluid samples from patients with neurocysticercosis.

scholarly journals Evaluation of an enzyme immunoassay for clinical diagnosis of neurocysticercosis in symptomatic patients

2010 ◽  
Vol 43 (6) ◽  
pp. 647-650 ◽  
Author(s):  
Reynaldo Mendes de Carvalho Junior ◽  
Dorcas Lamounier Costa ◽  
Savyo Carvalho Soares ◽  
Carlos Henrique Nery Costa
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Sensitivity And Specificity ◽  
Cerebral Spinal Fluid ◽  
Taenia Solium ◽  
Enzyme Linked Immunosorbent Assay ◽  
Parasitic Disease ◽  
Multivariate Logistic Regression ◽  
Human Central Nervous System ◽  
Elisa Kit

INTRODUCTION: Neurocysticercosis is an infection of the human central nervous system caused by the metacestode larvae of Taenia solium. Neurocysticercosis is the most common parasitic disease in developing countries. Epilepsy is the most common clinical manifestation. Difficulties in confirming the diagnosis motivated the evaluation of the enzyme-linked immunosorbent assay on cerebral spinal fluid (CSF). METHODS: Twenty-two patients with NCC and 44 control patients were studied. CSF was analyzed using a commercial ELISA kit developed for NCC. Sensitivity and specificity were measured and a multivariate logistic regression was performed. RESULTS: Sensitivity and specificity of ELISA were 31.8% and 100%, respectively, with accuracy of 77.3%. Only the size of the lesions proved to be important for performance of the test. CONCLUSIONS: The results showed that ELISA contributes to the diagnosis of neurocysticercosis if the result is negative or if the patient has a lesion of 2 cm or more.

scholarly journals Enzyme linked immunosorbent assay (ELISA) for the detection of IgG, IgM, IgE and IgA against Cysticercus cellulosae in cerebrospinal fluid of patients with neurocysticercosis

2002 ◽  
Vol 60 (2B) ◽  
pp. 400-405 ◽  
Author(s):  
Newton Satoru Odashima ◽  
Osvaldo Massaiti Takayanagui ◽  
José Fernando de Castro Figueiredo
Keyword(s):  
Cerebrospinal Fluid ◽  
Sensitivity And Specificity ◽  
Immunosorbent Assay ◽  
Neurological Diseases ◽  
Active Form ◽  
Clinical Manifestations ◽  
Enzyme Linked Immunosorbent Assay ◽  
Inactive Form ◽  
Cysticercus Cellulosae ◽  
Inflammation Signs

The objective of this study was to analyze different immunoglobulins classes (IgG, IgM, IgE and IgA) against Cysticercus cellulosae in the cerebrospinal fluid (CSF), through enzyme linked immunosorbent assay (ELISA), correlating them to clinical and tomographic profiles in patients with neurocysticercosis (NCC). Eighty-five specimens of CSF were obtained from 43 cases with NCC (26 with the active form and 17 with the inactive form) and from 42 patients with other neurological diseases. The inactive form of NCC presented a profile in CSF similar to the group without NCC. The active form of NCC presented elevation of specific immunoglobulins (IgG, IgM, IgE, and IgA) in decreasing order, with the highest values being detected among the cases with intraventricular cysts, or with inflammation signs in CSF or in those with multiple clinical manifestations. The highest sensitivity and specificity were obtained with ELISA-IgG (88.5% and 93.2%, respectively). This study confirmed the importance of ELISA in the immunologic diagnosis of NCC.

scholarly journals Frequency of serum anti-cysticercus antibodies in the population of a rural Brazilian community (Cássia dos Coqueiros, SP) determined by ELISA and immunoblotting using Taenia crassiceps antigens

2002 ◽  
Vol 44 (1) ◽  
pp. 7-12 ◽  
Author(s):  
Lúcia M. BRAGAZZA ◽  
Adelaide J. VAZ ◽  
Afonso D.C. PASSOS ◽  
Osvaldo M. TAKAYANAGUI ◽  
Paulo M. NAKAMURA ◽  
...  
Keyword(s):  
Public Health ◽  
Immunosorbent Assay ◽  
Taenia Solium ◽  
High Specificity ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Taenia Crassiceps ◽  
Municipal District ◽  
The Impact

Considering the impact of cysticercosis on public health, especially the neurologic form of the disease, neurocysticercosis (NC), we studied the frequency of positivity of anti-Taenia solium cysticercus antibodies in serum samples from 1,863 inhabitants of Cássia dos Coqueiros, SP, a municipal district located 80 km from Ribeirão Preto, an area considered endemic for cysticercosis. The 1,863 samples were tested by enzyme linked immunosorbent assay (ELISA) using an antigenic extract from Taenia crassiceps vesicular fluid (Tcra). The reactive and inconclusive ELISA samples were tested by immunoblotting. Of the 459 samples submitted to immunoblotting, 40 were strongly immunoreactive to the immunodominant 18 and 14 kD peptides. Considering the use of immunoblotting as confirmatory due to its high specificity, the anti-cysticercus serum prevalence in this population was 2.1%.

scholarly journals Specific Taenia crassiceps and Taenia solium Antigenic Peptides for Neurocysticercosis Immunodiagnosis Using Serum Samples

2000 ◽  
Vol 38 (1) ◽  
pp. 146-151
Author(s):  
Ednéia Casagranda Bueno ◽  
Adelaide José Vaz ◽  
Luís Dos Ramos Machado ◽  
JoséAntônio Livramento ◽  
Sílvia Regina Mielle
Keyword(s):  
Taenia Solium ◽  
Severe Infection ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Antigenic Peptides ◽  
Serum Samples ◽  
Immunoblotting Analysis ◽  
Taenia Crassiceps ◽  
The Central Nervous System ◽  
Paired Serum

ABSTRACT Neurocysticercosis (NC), i.e., the presence of the larval form of Taenia solium in tissues, is the most frequent and severe infection involving the central nervous system. Paired serum and cerebrospinal fluid (CSF) samples from patients with NC, CSF and serum samples from a control group, and serum samples from patients with other parasitoses were studied by enzyme-linked immunosorbent assay (ELISA) and by immunoblotting with Taenia crassiceps vesicular fluid antigen (Tcra) and Taenia solium total saline antigen (Tso) for the detection of immunoglobulin G antibodies. ELISAs carried out with the Tso and Tcra antigens showed 94.1 and 95.6% sensitivities, respectively, for the detection of antibodies in CSF and 70.6% and 91.2% sensitivities, respectively, for the detection of antibodies in serum, with 100% specificity for the detection of antibodies in CSF and 80% specificity for the detection of antibodies in serum for both antigens. On the basis of the reactivities of the peptides in the samples analyzed, the peptides of ≤23, 39, 85 to 77, and 97 kDa were found to be Tso specific by immunoblotting and the peptides of ≤62, 74, 109, 121, and 131 kDa were found to be Tcra specific. Tests with Tcra extract had higher sensitivities and more homogeneous results and permitted us to obtain the parasites easily. We suggest the use of Tcra ELISA for the study of serum and confirmation of the results for sera positive by an immunoblotting analysis in which specific peptides (e.g., peptides of 19 to 13 kDa) are detected.

IgY antibody and human neurocysticercosis: a novel approach on immunodiagnosis using Taenia crassiceps hydrophobic antigens

Parasitology ◽  
2019 ◽  
Vol 147 (2) ◽  
pp. 240-247 ◽  
Author(s):  
Guilherme M. P. Carrara ◽  
Gabriela B. Silva ◽  
Lucas S. Faria ◽  
Daniela S. Nunes ◽  
Vanessa S. Ribeiro ◽  
...  
Keyword(s):  
Immune Complexes ◽  
Sandwich Elisa ◽  
Taenia Solium ◽  
Antibody Test ◽  
Egg Yolk ◽  
Enzyme Linked Immunosorbent Assay ◽  
Taenia Crassiceps ◽  
Novel Approach ◽  
Human Sera ◽  
Diagnostic Values

AbstractHuman neurocysticercosis (NCC) is a worldwide neglected disease caused by Taenia solium metacestode and responsible for various complications and neurological disorders. This study aimed to evaluate the use of specific immunoglobulin Y (IgY) produced by laying hens immunized with a hydrophobic fraction of Taenia crassiceps metacestodes (hFTc) in NCC diagnosis. Egg yolk IgY antibodies were fractionated, purified and characterized. Enzyme-linked immunosorbent assay (ELISA) was carried out to evaluate the production kinetics and avidity maturation of anti-hFTc IgY antibodies throughout the IgY obtention process. Antigen recognition tests were carried out by Western blotting and immunofluorescence antibody test using purified and specific anti-hFTc IgY antibodies for detection of parasitic antigens of T. crassiceps and T. solium metacestodes. Sandwich ELISA was performed to detect circulating immune complexes formed by IgG and parasitic antigens in human sera. The results showed high diagnostic values (93.2% sensitivity and 94.3% specificity) for immune complexes detection in human sera with confirmed NCC. In conclusion, specific IgY antibodies produced from immunized hens with hFTc antigens were efficient to detect T. solium immune complexes in human sera, being an innovative and potential tool for NCC immunodiagnosis.

Evidence of human neurocysticercosis in Slovenia

Parasitology ◽  
2013 ◽  
Vol 141 (4) ◽  
pp. 547-553 ◽  
Author(s):  
BARBARA ŠOBA ◽  
BOJANA BEOVIĆ ◽  
ZALA LUŽNIK ◽  
MIHA SKVARČ ◽  
JERNEJ LOGAR
Keyword(s):  
Cerebrospinal Fluid ◽  
Neurological Disorders ◽  
Taenia Solium ◽  
Personal Data ◽  
Enzyme Linked Immunosorbent Assay ◽  
Seroprevalence Rate ◽  
Imaging Findings ◽  
Igg Antibodies ◽  
Brain Cysts ◽  
Insight Into

SUMMARYTo assess the prevalence of Taenia solium cysticercosis in patients with neurological disorders in Slovenia, serum/cerebrospinal fluid (CSF) samples from 348 suspected patients were collected between the beginning of January 2001 and the end of December 2012 and analysed serologically for the presence of anti-T. solium IgG antibodies. Of 20 patients whose samples tested positive or equivocal by enzyme-linked immunosorbent assay (ELISA), samples of 7 patients were confirmed positive by Western blot (WB). The overall seroprevalence rate of T. solium infection in patients with neurological disorders included in the study was 2.0%. Serological results of positive patients corresponded to clinical and/or imaging findings concerning their brain cysts. Based on their personal data, it was ascertained that neurocysticercosis (NCC) positive patients had immigrated or came to Slovenia from the former Yugoslav republics. Since the disease is believed not to be endemic in Slovenia we assume that all of the NCC-positive patients had acquired the infection before immigration to Slovenia or visiting or being visited by their relatives infected with an adult T. solium parasite. The present results represent the first insight into the prevalence of NCC in patients with neurological disorders in Slovenia.

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