scholarly journals Dried Blood Spots versus Sera for Detection of Rubella Virus-Specific Immunoglobulin M (IgM) and IgG in Samples Collected during a Rubella Outbreak in Peru

2007 ◽  
Vol 14 (11) ◽  
pp. 1522-1525 ◽  
Author(s):  
Rita F. Helfand ◽  
Cesar Cabezas ◽  
Emily Abernathy ◽  
Carlos Castillo-Solorzano ◽  
Ana Cecilia Ortiz ◽  
...  
Keyword(s):  
Filter Paper ◽  
Rubella Virus ◽  
Dried Blood Spots ◽  
Immunoglobulin M ◽  
Dried Blood Spot ◽  
Blood Spots ◽  
Specific Immunoglobulin ◽  
Blood Spot

ABSTRACT Most persons with rubella virus-specific immunoglobulin M (IgM)- or IgG-positive sera tested positive (98% [n = 178] and 99% [n = 221], respectively) using paired filter paper dried blood spot (DBS) samples, provided that DBS indeterminate results were called positive. For persons with IgM- or IgG-negative sera, 97% and 98%, respectively, were negative using DBS.

scholarly journals Development and validation of a LC-MS/MS method for ivermectin quantification in dried blood spots: application to a pharmacokinetic study inTrichuris trichiura-infected adults

2018 ◽  
Vol 10 (24) ◽  
pp. 2901-2909 ◽  
Author(s):  
Jessica D. Schulz ◽  
Anna Neodo ◽  
Jean T. Coulibaly ◽  
Jennifer Keiser
Keyword(s):  
Pharmacokinetic Study ◽  
Dried Blood Spots ◽  
Trichuris Trichiura ◽  
Dried Blood Spot ◽  
Plasma Samples ◽  
Blood Spots ◽  
Development And Validation ◽  
Blood Spot

Ivermectin was quantified in dried blood spot and plasma samples derived fromTrichuris trichiura-infected adults with a validated LC-MS/MS method.

Measurement of gonadotropins in dried blood spots

1994 ◽  
Vol 40 (3) ◽  
pp. 448-453 ◽  
Author(s):  
C M Worthman ◽  
J F Stallings
Keyword(s):  
Luteinizing Hormone ◽  
Filter Paper ◽  
Clinical Studies ◽  
Follicle Stimulating Hormone ◽  
Dried Blood Spots ◽  
Blood Spots ◽  
Low Concentrations ◽  
Serial Sampling ◽  
Highly Correlated ◽  
Blood Spot

Abstract We describe direct immunofluorometric assays for luteinizing hormone (hLH) and follicle-stimulating hormone (hFSH) in fingerstick blood spots dried on filter paper, based on modifications of commercially available kits. Determinations are made from 2.5-mm-diameter discs (3 microL of dried blood) punched out from blood spot standards and samples. Sample dose detection limits of the assays (IU/L) are 0.26 for LH and 0.13 for FSH, with mean interassay CVs of 11.6% (LH) and 7.8% (FSH) at low concentrations. Analytical recoveries of added hormone averaged 100% for LH and 95% for FSH. Clinical studies showed that values for blood spots (x) and directly assayed plasma (y) are highly correlated, so that results from blood spots can be converted directly to plasma equivalents, as follows: yLH = 0.07 + 1.90 xLH, and yFSH = 0.424 + 2.207 xFSH. These gonadotropins are stable in blood spots for at least a year under refrigeration; LH for at least 8 weeks and FSH 6 weeks at 22 degrees C; and both hormones for a week at 37 degrees C. These methods thus allow self-sampling, serial sampling, and mailing of specimens.

scholarly journals Measurement of total homocysteine in plasma and blood spots using liquid chromatography-tandem mass spectrometry: comparison with the plasma Abbott IMx method

2003 ◽  
Vol 40 (2) ◽  
pp. 161-165 ◽  
Author(s):  
Steven J. McCann ◽  
Scott Gillingwater ◽  
Brian G. Keevil ◽  
Donald P. Cooper ◽  
Michel R. Morris
Keyword(s):  
Sensitivity And Specificity ◽  
Plasma Concentrations ◽  
Dried Blood Spots ◽  
Dried Blood Spot ◽  
Tandem Mass ◽  
Blood Spots ◽  
Plasma Thcy ◽  
Total Homocysteine ◽  
Tandem Mass Spectrometric ◽  
Blood Spot

Background: Current sampling for total homocysteine (tHcy) is problematic, requiring plasma separation within 15 min. The aim of this study was to develop a liquid chromatographic-tandem mass spectrometric (LC-MS/MS) method for the measurement of tHcy in plasma and dried blood spots and to determine whether the dried blood spot concentration could be used to predict plasma concentrations of tHcy. Methods: LC-MS/MS methodology was optimized to measure tHcy in plasma and dried blood spots. Fifty blood samples collected from heart transplant patients were used to form dried blood spots and for plasma analysis. Plasma tHcy was also measured using the Abbott IMx1 method and values were compared to the tHcy concentrations determined in plasma and dried blood spots using LC-MS/MS methodology. Results: The plasma tHcy LC-MS/MS results compared well with the IMx values: LC-MS/MS=1·18(IMx)-0·44 ( r2=0·915). The within-batch precision ( n =10) of the plasma LC-MS/MS method was < 2·0% at 14·6 and 37·7 µmol/L, respectively; the between-batch precision ( n=10) was 5·0 and 8·0%, respectively, at these concentrations. The method was found to be sensitive down to 1 µmol/L and linear up to at least 100 µmol/L. Dried blood spot LC-MS/MS results were considerably lower than the plasma IMx values ( P < 0·0001): dried blood spot LC-MS/MS=0·33IMx+1·77 ( r2=0·682). The within-batch precision ( n=20) of the dried blood spot LC-MS/MS method was 7·3% and 4·7% at concentrations of 4·0 and 7·9 µmol/L, respectively; the between-batch precision was 12·6% and 7·9% at concentrations of 5·1 and 8·0 µmol/L, respectively. To assess whether dried blood spots are suitable as a screening test to predict plasma tHcy concentrations, arbitary cut-off levels were compared. If it is assumed that a plasma tHcy concentration of >15 µmol/L is raised, a dried blood spot result of >6·8 µmol/L has a sensitivity and specificity in detecting a raised plasma tHcy of 83·3% and 96·2%, respectively, and a positive and negative predictive value of 95% and 86%, respectively, with an efficiency of 90%. Use of a dried blood spot cut-off concentration of 6·2 µmol/L for predicting high plasma tHcy concentrations (above 15 µmol/L) has a sensitivity and specificity of 95·8% and 73·1%, respectively, positive and negative predictive values of 76% and 95%, respectively, and an efficiency of 84%. Conclusions: We have developed a precise and accurate LC-MS/MS method for measuring plasma tHcy concentrations, which uses a small volume of plasma and is suitable for routine use. A satisfactory LC-MS/MS method for the measurement of tHcy in dried blood spots was also developed; this method might be useful in routine screening for raised plasma concentrations of tHcy.

scholarly journals Performance of dried blood spot (DBS) PUNCHER and dried blood spots to measure HIV-1 viral load

2016 ◽  
Vol 10 (35) ◽  
pp. 1439-1443
Author(s):  
Dagnra Anoumou ◽  
Salou Mounerou ◽  
Ehlan Amivi ◽  
Konou Ahouefa ◽  
Ouro-Medeli Alassane ◽  
...  
Keyword(s):  
Viral Load ◽  
Dried Blood Spots ◽  
Dried Blood Spot ◽  
Blood Spots ◽  
Hiv 1 ◽  
Blood Spot

scholarly journals Effect of Dried Blood Spot Quality on Newborn Screening Analyte Concentrations and Recommendations for Minimum Acceptance Criteria for Sample Analysis

2016 ◽  
Vol 62 (3) ◽  
pp. 466-475 ◽  
Author(s):  
Roanna S George ◽  
Stuart J Moat
Keyword(s):  
Newborn Screening ◽  
Filter Paper ◽  
Statistical Significance ◽  
False Negative ◽  
Dried Blood Spots ◽  
Sample Analysis ◽  
Acceptance Criteria ◽  
Blood Spots ◽  
Spot Quality ◽  
Blood Spot

Abstract BACKGROUND The analysis of dried blood spots has been used routinely for newborn screening since the early 1970s, and the number of disorders screened has expanded substantially in recent years. However, there is a lack of evidence regarding minimum blood spot quality acceptance criteria for sample analysis. METHODS Blood pools were spiked with phenylalanine, tyrosine, leucine, methionine, octanoylcarnitine, decanoylcarnitine, isovalerylcarnitine, glutarylcarnitine, thyroid-stimulating hormone, and immunoreactive trypsinogen to concentrations at the analytical cutoffs used in UK screening protocols. We evaluated the effect of sample volume applied to the card (10, 20, 50, 75, and 100 μL), punch location (central vs peripheral), and sample quality (double layering, applying blood to both sides of the filter paper, multispotting, applying insufficient sample, and compressing the sample after application). RESULTS Compression of blood spots produced significantly lower results (14%–44%) for all analytes measured (P &lt; 0.001). Smaller blood spots produced significantly lower results (15%–24% for 10-μL vs 50-μL sample size) for all analytes at all concentrations measured (P &lt; 0.001). Results obtained from peripheral punches were higher than those from a central punch, although this did not reach statistical significance for all analytes. Insufficient and multispotted samples demonstrated heterogeneous results. CONCLUSIONS All blood spots containing ≤20 μL (blood spot diameter &lt;8 mm), those in which blood has not fully penetrated the filter paper, and all samples with evidence of compression should be rejected, since there is a risk of producing false-negative results.

scholarly journals Detection of ctDNA from dried blood spots after DNA size selection

2019 ◽  
Author(s):  
Katrin Heider ◽  
Jonathan C. M. Wan ◽  
James Hall ◽  
Samantha Boyle ◽  
Irena Hudecova ◽  
...  
Keyword(s):  
Copy Number ◽  
Fragment Size ◽  
Dried Blood Spots ◽  
Dried Blood Spot ◽  
Copy Number Alterations ◽  
Sample Collection ◽  
Cell Free Dna ◽  
Blood Spots ◽  
Free Dna ◽  
Blood Spot

AbstractRecent advances in the research and clinical applications of circulating tumour DNA (ctDNA) is limited by practical considerations of sample collection. Whole genome sequencing (WGS) is increasingly used for analysis of ctDNA, identifying copy-number alterations, fragment size patterns, and other genomic features. We hypothesised that low-depth WGS data may be generated from minute amounts of cell-free DNA, and that fragment-size selection may be effective to remove contaminating genomic DNA (gDNA) from small volumes of blood. There are practical advantages to using dried blood spots as these are easier to collect, facilitate serial sampling, and support novel study designs in prospective human studies, animal models and expand the utilisation of archival samples by the removal of gDNA in small volumes. We therefore developed a protocol for the isolation and analysis of cell-free DNA from dried blood spots. Analysing a dried blood spot of 50μL frozen whole blood from a patient with melanoma, we identified ctDNA based on tumour-specific somatic copy-number alterations, and found a fragment size profile similar to that observed in plasma DNA processed by traditional methods. We extended this approach to detect tumour-derived cell-free DNA in a dried blood spot from a mouse xenograft model and were able to identify ctDNA from the originally grafted ascites. Together, our data suggests that ctDNA can be detected and monitored in dried blood spots. This will enable new approaches for sample collection from patients andin vivomodels.

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