scholarly journals Genetic evidence that promoter P2 is the physiologically significant promoter for the pyrBI operon of Escherichia coli K-12.

1993 ◽  
Vol 175 (8) ◽  
pp. 2363-2369 ◽  
Author(s):  
C Liu ◽  
J P Donahue ◽  
L S Heath ◽  
C L Turnbough
Keyword(s):  
Escherichia Coli ◽  
Genetic Evidence ◽  
K 12

scholarly journals Genetic Evidence for an Interaction of the UbiG O-Methyltransferase with UbiX in Escherichia coli Coenzyme Q Biosynthesis

2006 ◽  
Vol 188 (17) ◽  
pp. 6435-6439 ◽  
Author(s):  
Melissa Gulmezian ◽  
Haitao Zhang ◽  
George T. Javor ◽  
Catherine F. Clarke
Keyword(s):  
Escherichia Coli ◽  
Mutant Strain ◽  
Coenzyme Q ◽  
Genetic Evidence ◽  
Deficient Mutant ◽  
Gene Encoding ◽  
K 12

ABSTRACT IS16 is a thiol-sensitive, Q-deficient mutant strain of Escherichia coli. Here, we show that IS16 harbors a mutation in the ubiG gene encoding a methyltransferase required for two O-methylation steps of Q biosynthesis. Complementation of IS16 with either ubiG or ubiX K-12 reverses this phenotype, suggesting that UbiX may interact with UbiG.

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