scholarly journals Biochemical and genetic analyses of a catalase from the anaerobic bacterium Bacteroides fragilis.

1995 ◽  
Vol 177 (11) ◽  
pp. 3111-3119 ◽  
Author(s):  
E R Rocha ◽  
C J Smith
Keyword(s):  
Anaerobic Bacterium ◽  
Bacteroides Fragilis ◽  
Genetic Analyses

Crystal Structure of a Transcarbamylase-like Protein from the Anaerobic Bacterium Bacteroides fragilis at 2.0Å Resolution

2002 ◽  
Vol 320 (4) ◽  
pp. 899-908 ◽  
Author(s):  
Dashuang Shi ◽  
Rene Gallegos ◽  
Joseph DePonte ◽  
Hiroki Morizono ◽  
Xiaolin Yu ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Anaerobic Bacterium ◽  
Bacteroides Fragilis

scholarly journals Detection of Intrastrain Antigenic Variation of Bacteroides fragilis Surface Polysaccharides by Monoclonal Antibody Labelling

1999 ◽  
Vol 67 (9) ◽  
pp. 4346-4351 ◽  
Author(s):  
Sheila Patrick ◽  
Deirdre Gilpin ◽  
Leanne Stevenson
Keyword(s):  
Monoclonal Antibody ◽  
Anaerobic Bacterium ◽  
Antigenic Variation ◽  
Bacteroides Fragilis ◽  
Virulence Determinants ◽  
Immunomagnetic Beads ◽  
Individual Strain ◽  
Surface Polysaccharides ◽  
Immunodiagnostic Tests ◽  
Antibody Labelling

ABSTRACT Bacteroides fragilis is a constituent of the normal resident microbiota of the human intestine and is the gram-negative obligately anaerobic bacterium most frequently isolated from clinical infection. Surface polysaccharides are implicated as potential virulence determinants. We present evidence of within strain immunochemical variation of surface polysaccharides in populations that are noncapsulate by light microscopy as determined by monoclonal antibody labelling. Expression of individual epitopes can be enriched from a population of an individual strain by use of immunomagnetic beads. Also, individual colonies in which either >94% or <7% of the bacteria carry an individual epitope retain this level of expression when subcultured into broth. In broth cultures where >94% of the bacteria carry a given epitope, there is no enrichment for other epitopes recognized by different polysaccharide-specific monoclonal antibodies. This intrastrain variation has important implications for the development of potential vaccines or immunodiagnostic tests.

Morphologic alterations in bacteroides fragilis following single and combinant beta-lactam exposure

1986 ◽  
Vol 44 ◽  
pp. 304-305
Author(s):  
Michael P. Goheen ◽  
Charles E. Edmiston
Keyword(s):  
Bacteroides Fragilis ◽  
Synergistic Activity ◽  
Beta Lactam ◽  
Clinical Strains ◽  
Drug Activity ◽  
Obligate Anaerobic ◽  
Morphologic Changes

The synergistic activity of antimicrobial combinants against aerobic and facultative microorganisms has been well documented. in comparison, few studies have been performed using obligate anaerobic isolates and antimicrobial combinants. For this study clinical strains of Bacteroides fragilis(BF) were selected to investigate both single/combinant drug activity and cellular morphologic changes when BF is exposed to Imipenem (I), Piperacillin (P), Cefpimizole (C), Imipenem/Piperacillin (I+P), and Imipenem/Cefpimizole (I+C).

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